In hepatocytes from starved streptozocin-induced diabetic rats, vanadate increases the glycolytic flux because it raises the levels of fructose-2,6-bisphosphate (Fru-2,6-P2), the main regulatory metabolite of this pathway. This effect of vanadate on Fru-2,6-P2 levels is time and dose dependent, and it remains in cells incubated in a calcium-depleted medium. Vanadate is also able to counteract the decrease on Fru-2,6-P2 levels produced by glucagon, colforsin, or exogenous cAMP. However, vanadate does not modify 6-phosphofructo-2-kinase and pyruvate kinase activities, but it does counteract the inactivation of these enzymes induced by glucagon. Likewise, Fru-2,6-P2ase activity is also not affected by vanadate. In addition, vanadate is able to increase the production of both lactate and CO2 in hepatocytes from streptozocin-induced diabetic rats incubated in the presence of glucose in the medium. Vanadate behaves as a glycolytic effector in these cells, and this effect may be related to its ability to normalize blood glucose levels in diabetic animals.
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Original Articles|
October 01 1991
Activation by Vanadate of Glycolysis in Hepatocytes From Diabetic Rats
Joan E Rodríguez-Gil;
Joan E Rodríguez-Gil
Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Autonomous University of Barcelona; and the Department of Biochemistry and Physiology, School of Chemistry, University of Barcelona
Barcelona, Spain
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Anna M Gómez-Foix;
Anna M Gómez-Foix
Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Autonomous University of Barcelona; and the Department of Biochemistry and Physiology, School of Chemistry, University of Barcelona
Barcelona, Spain
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Cristina Fillat;
Cristina Fillat
Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Autonomous University of Barcelona; and the Department of Biochemistry and Physiology, School of Chemistry, University of Barcelona
Barcelona, Spain
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Fàtima Bosch;
Fàtima Bosch
Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Autonomous University of Barcelona; and the Department of Biochemistry and Physiology, School of Chemistry, University of Barcelona
Barcelona, Spain
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Joan J Guinovart
Joan J Guinovart
Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Autonomous University of Barcelona; and the Department of Biochemistry and Physiology, School of Chemistry, University of Barcelona
Barcelona, Spain
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Address correspondence and reprint requests to Joan E. Rodríguez-Gil, Department de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autànoma de Barcelona, 08193 Bellaterra (Barcelona), Spain
Diabetes 1991;40(10):1355–1359
Article history
Received:
November 09 1990
Revision Received:
May 03 1991
Accepted:
May 03 1991
Citation
Joan E Rodríguez-Gil, Anna M Gómez-Foix, Cristina Fillat, Fàtima Bosch, Joan J Guinovart; Activation by Vanadate of Glycolysis in Hepatocytes From Diabetic Rats. Diabetes 1 October 1991; 40 (10): 1355–1359. https://doi.org/10.2337/diab.40.10.1355
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