Both exercise training and dietary manipulation (increasing ω-3/ω-6 fat ratio) can ameliorate insulin resistance caused by a high-fat diet in rats. We determined whether alterations in the expression of the insulin-regulatable (IR) and/or HepG2 glucose-transporter (GT) mRNAs were similarly affected. There was a significantly higher level of IRGT mRNA in skeletal muscle from exercise-trained versus sedentary high-fat–fed rats (27% increase, P < 0.01). This difference is consistent with previously reported increases in muscle insulin-mediated glucose uptake. Skeletal muscle HepG2GT mRNA was too low to detect any training effect, but there was a tendency toward higher levels with training in cardiac muscle. In contrast, dietary manipulation, previously shown to lead to a much greater increase (100–300%) in muscle insulin-mediated glucose uptake, did not change IRGT or HepG2GT mRNA in skeletal muscle or heart. Thus, both dietary manipulation and exercise training increase insulin-stimulated glucose uptake in skeletal muscle, but only exercise training increases IRGT mRNA. Therefore, exercise training apparently increases GT production, whereas dietary manipulation improves glucose transport in skeletal muscle by other mechanisms.
Skip Nav Destination
Article navigation
Original Articles|
February 01 1991
Effects of Exercise Training and Dietary Manipulation on Insulin-Regulatable Glucose-Transporter mRNA in Rat Muscle
Samantha A Wake;
Samantha A Wake
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
Judith A Sowden;
Judith A Sowden
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
Leonard H Storlien;
Leonard H Storlien
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
David E James;
David E James
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
Peter W Clark;
Peter W Clark
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
John Shine;
John Shine
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
Donald J Chisholm;
Donald J Chisholm
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
Edward W Kraegen
Edward W Kraegen
Garvan Institute of Medical Research, St. Vincent's Hospital
Darlinghurst, New South Wales, Australia
; and Department of Cell Biology and Physiology, Washington University School of Medicine
St. Louis, Missouri
Search for other works by this author on:
Address correspondence and reprint requests to Dr. E.W. Kraegen, Garvan Institute, St. Vincent's Hospital, Darlinghurst, NSW 2010, Australia.
Diabetes 1991;40(2):275–279
Article history
Received:
February 02 1990
Revision Received:
October 09 1990
Accepted:
October 09 1990
PubMed:
1991574
Citation
Samantha A Wake, Judith A Sowden, Leonard H Storlien, David E James, Peter W Clark, John Shine, Donald J Chisholm, Edward W Kraegen; Effects of Exercise Training and Dietary Manipulation on Insulin-Regulatable Glucose-Transporter mRNA in Rat Muscle. Diabetes 1 February 1991; 40 (2): 275–279. https://doi.org/10.2337/diab.40.2.275
Download citation file:
80
Views