The effects of different glucose concentrations on the expression of extracellular matrix genes were examined in primary cell cultures initiated from sciatic nerves of nondiabetic Sprague-Dawley rats. The cells were incubated in medium containing 5.5, 15, or 25 mM D-glucose, and the expression of type I and IV collagens, laminin, and fibronectin genes was examined at mRNA steady-state levels by Northern hybridizations. Incubation of cell cultures, consisting of Schwann cells, perineurial cells, and fibroblasts, in high glucose concentrations (15 or 25 mM D-glucose) resulted in elevation of pro-α 1(IV), pro-α 2(IV), and pro-α 1(I) collagen chain and fibronectin mRNAs after only 3 days of incubation, whereas laminin B2 chain mRNA levels appeared unaltered. These observations suggest that hyperglycemia may partially contribute to basement membrane thickening in peripheral nerves of diabetic individuals by increasing the expression of genes coding for basement membrane components, e.g., type IV collagen.
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Original Articles|
May 01 1991
Increased Matrix Gene Expression by Glucose in Rat Neural Connective Tissue Cells in Culture
Päivi Muona;
Päivi Muona
Departments of Dermatology and Biochemistry and Molecular Biology, Jefferson Medical College, and Section of Molecular Dermatology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University
Philadelphia, Pennsylvania
; and the Department of Medical Biochemistry, University of Turku
Turku, Finland
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Juha Peltonen;
Juha Peltonen
Departments of Dermatology and Biochemistry and Molecular Biology, Jefferson Medical College, and Section of Molecular Dermatology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University
Philadelphia, Pennsylvania
; and the Department of Medical Biochemistry, University of Turku
Turku, Finland
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Sirkku Jaakkola;
Sirkku Jaakkola
Departments of Dermatology and Biochemistry and Molecular Biology, Jefferson Medical College, and Section of Molecular Dermatology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University
Philadelphia, Pennsylvania
; and the Department of Medical Biochemistry, University of Turku
Turku, Finland
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Jouni Uitto
Jouni Uitto
Departments of Dermatology and Biochemistry and Molecular Biology, Jefferson Medical College, and Section of Molecular Dermatology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University
Philadelphia, Pennsylvania
; and the Department of Medical Biochemistry, University of Turku
Turku, Finland
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Address correspondence and reprint requests to Jouni J. Uitto, MD, PhD, Department of Dermatology, Thomas Jefferson University, 1020 Locust Street, Room M-46, Jefferson Alumni Hall, Philadelphia, PA 19107.
Diabetes 1991;40(5):605–611
Article history
Received:
June 12 1990
Revision Received:
December 26 1990
Accepted:
December 26 1990
PubMed:
2022305
Citation
Päivi Muona, Juha Peltonen, Sirkku Jaakkola, Jouni Uitto; Increased Matrix Gene Expression by Glucose in Rat Neural Connective Tissue Cells in Culture. Diabetes 1 May 1991; 40 (5): 605–611. https://doi.org/10.2337/diab.40.5.605
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