Human progalanin cDNA was cloned with polymerase chain reaction techniques. The cDNA sequence predicts that the human form of galanin has a substitution of the glycine residue found at position 30 in other species and thus is likely to retain this residue during posttranslational processing and not be amidated at the COOH terminus. Furthermore, the cDNA sequence predicts three additional amino acid substitutions compared with known galanins. Human galanin was synthesized, and its bioactivity was compared with porcine and rat galanin based on inhibition of insulin release from a glucose-responsive rat insulinoma (RIN) cell line. Human galanin inhibited glucose-stimulated insulin secretion in adose-dependent manner in RIN cells. Human, porcine, and rat galanin exhibited similar activity with ED50 <1 nM.
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Original Articles|
January 01 1992
Sequence of Human Galanin and Its Inhibition of Glucose-Stimulated Insulin Secretion From RIN Cells
Gary L McKnight;
Gary L McKnight
ZymoGenetics, Inc.; R.H. Williams Laboratory, Department of Medicine, University of Washington; and the Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Seattle Veteran's Administration Medical Center and University of Washington
Seattle, Washington
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Allan E Karlsen;
Allan E Karlsen
ZymoGenetics, Inc.; R.H. Williams Laboratory, Department of Medicine, University of Washington; and the Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Seattle Veteran's Administration Medical Center and University of Washington
Seattle, Washington
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Stephan Kowalyk;
Stephan Kowalyk
ZymoGenetics, Inc.; R.H. Williams Laboratory, Department of Medicine, University of Washington; and the Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Seattle Veteran's Administration Medical Center and University of Washington
Seattle, Washington
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Shannon L Mathewes;
Shannon L Mathewes
ZymoGenetics, Inc.; R.H. Williams Laboratory, Department of Medicine, University of Washington; and the Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Seattle Veteran's Administration Medical Center and University of Washington
Seattle, Washington
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Paul O Sheppard;
Paul O Sheppard
ZymoGenetics, Inc.; R.H. Williams Laboratory, Department of Medicine, University of Washington; and the Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Seattle Veteran's Administration Medical Center and University of Washington
Seattle, Washington
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Patrick J O'Hara;
Patrick J O'Hara
ZymoGenetics, Inc.; R.H. Williams Laboratory, Department of Medicine, University of Washington; and the Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Seattle Veteran's Administration Medical Center and University of Washington
Seattle, Washington
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Gerald J Taborsky, Jr
Gerald J Taborsky, Jr
ZymoGenetics, Inc.; R.H. Williams Laboratory, Department of Medicine, University of Washington; and the Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, Seattle Veteran's Administration Medical Center and University of Washington
Seattle, Washington
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Address correspondence and reprint requests to Gary L. McKnight, ZymoGenetics Inc., 4225 Roosevelt Way Northeast, Seattle, WA 98105.
Diabetes 1992;41(1):82–87
Article history
Received:
June 08 1991
Revision Received:
September 30 1991
Accepted:
September 30 1991
PubMed:
1370155
Citation
Gary L McKnight, Allan E Karlsen, Stephan Kowalyk, Shannon L Mathewes, Paul O Sheppard, Patrick J O'Hara, Gerald J Taborsky; Sequence of Human Galanin and Its Inhibition of Glucose-Stimulated Insulin Secretion From RIN Cells. Diabetes 1 January 1992; 41 (1): 82–87. https://doi.org/10.2337/diab.41.1.82
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