A recent study by C.F. Burant et al. (13) demonstrates that GLUT5 is a high-affinity fructose transporter with a much lower capacity to transport glucose. To characterize the potential role of GLUT5 in fructose and glucose transport in insulin-sensitive tissues, we investigated the distribution and insulin-stimulated translocation of the GLUTS protein in human tissues by immunoblotting with an antibody to the COOH-terminus of the human GLUTS sequence. GLUTS was detected in postnuclear membranes from the small intestine, kidney, heart, four different skeletal muscle groups, and the brain, and in plasma membranes from adipocytes. Cytochalasin-B photolabeled a 53,000-Mr protein in small intestine membranes that was immunoprecipitated by the GLUT5 antibody; labeling was inhibited by D- but not L-glucose. N-glycanase treatment resulted in a band of 45,000 Mr in all tissues. Plasma membranes were prepared from isolated adipocytes from 5 nonobese and 4 obese subjects. Incubation of adipocytes from either group with 7 nM insulin did not recruit GLUT5 to the plasma membrane, in spite of a 54% insulin-stimulated increase in GLUT4 in nonobese subjects. Thus, GLUT5 appears to be a constitutive sugar transporter that is expressed in many tissues. Further studies are needed to define its overall contribution to fructose and glucose transport in insulin-responsive tissues and brain.
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October 01 1992
Human Small Intestine Facilitative Fructose/Glucose Transporter (GLUT5) Is Also Present in Insulin-Responsive Tissues and Brain: Investigation of Biochemical Characteristics and Translocation
Peter R Shepherd;
Peter R Shepherd
Charles A. Dana Research Institute and Harvard-Thorndike Laboratory of Beth Israel Hospital, Department of Medicine, Beth Israel Hospital and Harvard Medical School
Boston, Massachusetts
Department of Biochemistry, University of Glasgow
Glasgow, Scotland
Department of Medicine, University of Goteborg
Goteborg, Sweden
Pfizer Central Research
Groton, Connecticut
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E Michael Gibbs;
E Michael Gibbs
Charles A. Dana Research Institute and Harvard-Thorndike Laboratory of Beth Israel Hospital, Department of Medicine, Beth Israel Hospital and Harvard Medical School
Boston, Massachusetts
Department of Biochemistry, University of Glasgow
Glasgow, Scotland
Department of Medicine, University of Goteborg
Goteborg, Sweden
Pfizer Central Research
Groton, Connecticut
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Christian Wesslau;
Christian Wesslau
Charles A. Dana Research Institute and Harvard-Thorndike Laboratory of Beth Israel Hospital, Department of Medicine, Beth Israel Hospital and Harvard Medical School
Boston, Massachusetts
Department of Biochemistry, University of Glasgow
Glasgow, Scotland
Department of Medicine, University of Goteborg
Goteborg, Sweden
Pfizer Central Research
Groton, Connecticut
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Gwyn W Gould;
Gwyn W Gould
Charles A. Dana Research Institute and Harvard-Thorndike Laboratory of Beth Israel Hospital, Department of Medicine, Beth Israel Hospital and Harvard Medical School
Boston, Massachusetts
Department of Biochemistry, University of Glasgow
Glasgow, Scotland
Department of Medicine, University of Goteborg
Goteborg, Sweden
Pfizer Central Research
Groton, Connecticut
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Barbara B Kahn
Barbara B Kahn
Charles A. Dana Research Institute and Harvard-Thorndike Laboratory of Beth Israel Hospital, Department of Medicine, Beth Israel Hospital and Harvard Medical School
Boston, Massachusetts
Department of Biochemistry, University of Glasgow
Glasgow, Scotland
Department of Medicine, University of Goteborg
Goteborg, Sweden
Pfizer Central Research
Groton, Connecticut
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Address correspondence and reprint requests to Barbara B. Kahn, MD, Diabetes Unit/Beth Israel Hospital, 330 Brookline Ave., Boston, MA 02215.
Diabetes 1992;41(10):1360–1365
Article history
Received:
June 08 1992
Revision Received:
July 16 1992
Accepted:
July 16 1992
PubMed:
1397712
Citation
Peter R Shepherd, E Michael Gibbs, Christian Wesslau, Gwyn W Gould, Barbara B Kahn; Human Small Intestine Facilitative Fructose/Glucose Transporter (GLUT5) Is Also Present in Insulin-Responsive Tissues and Brain: Investigation of Biochemical Characteristics and Translocation. Diabetes 1 October 1992; 41 (10): 1360–1365. https://doi.org/10.2337/diab.41.10.1360
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