The goal of the Fourth International Workshop for Standardization of ICA Measurements was to determine the specificity of ICA assays and their ability to distinguish between control sera (n = 57) and sera from IDDM-related individuals—representing relatives of IDDM patients (n = 21), healthy individuals who later developed IDDM (n = 8), or newly diagnosed IDDM patients (n = 23). Results from 28 laboratories were analyzed. The mean specificity (percentage of control sera reported as negative) among 27 laboratories was 91%, including 6 laboratories with 100% specificity. Nevertheless, 78% of laboratories found at least one control sample >0 JDF U. Among samples from first-degree relatives, the mean concordance was 86%, including three sera found negative (0 JDF U) by all laboratories. Among individuals who later developed diabetes, the mean concordance was 93%, with two sera found positive by 100% of laboratories. In sera from newly diagnosed IDDM patients, the mean concordance was 82%. Three sera were found positive and one serum negative by all laboratories. The JDF U of the sera considered to be positive were significantly greater than each laboratory's average for the controls. In conclusion, the results from laboratories participating in the Fourth International ICA Workshop demonstrated excellent specificity, good concordance, and an ability to separate control sera from defined, IDDM-related subjects.
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Original Articles|
December 01 1992
Improved Specificity of ICA Assays in the Fourth International Immunology of Diabetes Serum Exchange Workshop Free
Carla J Greenbaum;
Carla J Greenbaum
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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Jerry P Palmer;
Jerry P Palmer
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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S Nagataki;
S Nagataki
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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Y Yamaguchi;
Y Yamaguchi
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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J L Molenaar;
J L Molenaar
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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W A M Van Beers;
W A M Van Beers
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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Noel K Maclaren;
Noel K Maclaren
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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Åke Lernmark;
Åke Lernmark
Department of Medicine, University of Washington
Seattle, Washington
Nagasaki University School of Medicine
Nagasaki, Japan
Klinik Immunologie
Kelft, The Netherlands
the University of Florida
Gainesville, Florida
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Participating Laboratories
Participating Laboratories
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Address correspondence and reprint requests to Carla J. Greenbaum, MD, Veterans Affairs Medical Center, 1660 South Columbian Way, No. 111, Seattle, WA 98108.
Diabetes 1992;41(12):1570–1574
Article history
Received:
March 13 1992
Revision Received:
July 16 1992
Accepted:
July 16 1992
PubMed:
1446798
Citation
Carla J Greenbaum, Jerry P Palmer, S Nagataki, Y Yamaguchi, J L Molenaar, W A M Van Beers, Noel K Maclaren, Åke Lernmark, Participating Laboratories; Improved Specificity of ICA Assays in the Fourth International Immunology of Diabetes Serum Exchange Workshop. Diabetes 1 December 1992; 41 (12): 1570–1574. https://doi.org/10.2337/diab.41.12.1570
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