Both a high level of D-glucose in the medium and serum from a diabetic rat can induce neural-tube fusion defects and growth retardation in cultured mouse and rat embryos. To test our hypothesis that a deficiency of PGs may be involved in the mechanism of hyperglycemia- and diabetic serum-induced teratogenesis and growth retardation, we added PGE2 to the medium of a whole mouse embryo culture containing either normal rat serum and 52.7 mM D-glucose (hyperglycemic) or diabetic rat serum and 22.2 mM D-glucose (diabetic). After a 24-h culture, 94% of hyperglycemic embryos and 81% of diabetic embryos had neural-tube fusion defects; in addition, the number of somites, the morphological score, and the protein content of the embryos were significantly lower than those of controls. Supplementing the medium with PGE2 at concentrations of 0.028–28.4 nM (hyperglycemic) or 28.4 nM (diabetic) significantly reduced the incidence of neural-tube defects and increased the number of somites, the morphological score, and the protein content. These results strongly support the hypothesis that the teratogenicity of diabetic serum, as well as the teratogenic action of hyperglycemic culture, are mediated through a deficiency of PGs.
Skip Nav Destination
Article navigation
Original Articles|
December 01 1992
PGE2 Prevents Anomalies Induced by Hyperglycemia or Diabetic Serum in Mouse Embryos
Motoko P Goto;
Motoko P Goto
Craniofacial Center, Department of Pediatrics, University of Illinois College of Medicine
Chicago, Illinois
Search for other works by this author on:
Allen S Goldman;
Allen S Goldman
Craniofacial Center, Department of Pediatrics, University of Illinois College of Medicine
Chicago, Illinois
Search for other works by this author on:
Michael R Uhing
Michael R Uhing
Craniofacial Center, Department of Pediatrics, University of Illinois College of Medicine
Chicago, Illinois
Search for other works by this author on:
Address correspondence and reprint requests to Motoko P. Goto, MD, PhD, Pediatrics (M/C 588), University of Illinois, 840 South Wood Street, Chicago, IL 60680.
Diabetes 1992;41(12):1644–1650
Article history
Received:
February 18 1992
Revision Received:
June 11 1992
Accepted:
June 11 1992
PubMed:
1446806
Citation
Motoko P Goto, Allen S Goldman, Michael R Uhing; PGE2 Prevents Anomalies Induced by Hyperglycemia or Diabetic Serum in Mouse Embryos. Diabetes 1 December 1992; 41 (12): 1644–1650. https://doi.org/10.2337/diab.41.12.1644
Download citation file:
49
Views