Because a restricted repertoire of T-cell receptor (TCR) Vβp gene expression has been reported in other autoimmune diseases, the possibility of similarly restricted Vα gene expression by T-cell infiltrates of NOD mouse islets was examined. With isolated islets from 4- to 12-wk-old NOD mice, a prospective polymerase chain reaction analysis with 18 Vβ-specific oligonucleotide primers was performed on the noncloned and unexpanded islet-infiltrating T cells. The methodology used permitted the detection of a minimum of 50 T cells. In contrast to the restricted TCR Vβ gene usage reported for other autoimmune diseases, infiltrates of even the youngest mice were characterized by expression of multiple Vβ gene segments.
Multiple TCR Vβ Usage by Infiltrates of Young NOD Mouse Islets of Langerhans: A Polymerase Chain Reaction Analysis
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Simon H Waters, John J O'Neil, David T Melican, Michael C Appel; Multiple TCR Vβ Usage by Infiltrates of Young NOD Mouse Islets of Langerhans: A Polymerase Chain Reaction Analysis. Diabetes 1 March 1992; 41 (3): 308–312. https://doi.org/10.2337/diab.41.3.308
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