Amylin is a 37–amino acid peptide synthesized in the pancreatic β-cell and cosecreted with insulin. In situ hybridization of nondiabetic rat pancreas shows that insulin and amylin RNA are both localized within the islet of Langerhans in a similar distribution. After 12 days of insulin-induced hypoglycemia (mean blood glucose 3.0 ± 0.4 mM [54 ± 8 mg/dl]), both insulin and amylin RNA fell > 95%. However, maintenance of euglycemia by simultaneous infusion of glucose with insulin did not suppress insulin or amylin RNA. Fasting suppressed amylin and insulin secretion from the isolated, perfused pancreas 70 and 58%, respectively, and with refeeding, secretion rates recovered to fed levels. Despite these changes in the rates of secretion, the relative ratio of amylin to insulin was not significantly different in fed, fasted, or refed rats. The molar ratio of insulin to amylin was estimated to be 100:2.3–2.6. Both insulin and amylin RNA was suppressed ∼ 50% in response to fasting. Thus, although the absolute amounts of insulin and amylin change substantially under the conditions tested, the relative amounts of these peptides do not change.
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Original Articles|
April 01 1992
Coordinate Regulation of Amylin and Insulin Expression in Response to Hypoglycemia and Fasting
Tausif Alam;
Tausif Alam
Center for Diabetes Research, Gifford Laboratories, Departments of Internal Medicine and Molecular Genetics, University of Texas Southwestern Medical Center, Veteran's Administration Medical Center
Dallas, Texas
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Ling Chen;
Ling Chen
Center for Diabetes Research, Gifford Laboratories, Departments of Internal Medicine and Molecular Genetics, University of Texas Southwestern Medical Center, Veteran's Administration Medical Center
Dallas, Texas
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Atsushi Ogawa;
Atsushi Ogawa
Center for Diabetes Research, Gifford Laboratories, Departments of Internal Medicine and Molecular Genetics, University of Texas Southwestern Medical Center, Veteran's Administration Medical Center
Dallas, Texas
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Jonathan D Leffert;
Jonathan D Leffert
Center for Diabetes Research, Gifford Laboratories, Departments of Internal Medicine and Molecular Genetics, University of Texas Southwestern Medical Center, Veteran's Administration Medical Center
Dallas, Texas
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Roger H Unger;
Roger H Unger
Center for Diabetes Research, Gifford Laboratories, Departments of Internal Medicine and Molecular Genetics, University of Texas Southwestern Medical Center, Veteran's Administration Medical Center
Dallas, Texas
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Kenneth L Luskey
Kenneth L Luskey
Center for Diabetes Research, Gifford Laboratories, Departments of Internal Medicine and Molecular Genetics, University of Texas Southwestern Medical Center, Veteran's Administration Medical Center
Dallas, Texas
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Address correspondence and reprint requests to Kenneth L. Luskey, MD, California Biotechnology Inc., 2450 Bayshore Parkway, Mountain View, CA 94043.
Diabetes 1992;41(4):508–514
Article history
Received:
July 15 1991
Revision Received:
January 03 1992
Accepted:
January 03 1992
PubMed:
1607075
Citation
Tausif Alam, Ling Chen, Atsushi Ogawa, Jonathan D Leffert, Roger H Unger, Kenneth L Luskey; Coordinate Regulation of Amylin and Insulin Expression in Response to Hypoglycemia and Fasting. Diabetes 1 April 1992; 41 (4): 508–514. https://doi.org/10.2337/diab.41.4.508
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