In vitro glomerular collagen synthesis and its response to various concentrations of transforming growth factor-β1 were studied in normal and diabetic rats. TGF-β1 increased collagen synthesis in normal glomeruli in a dose-dependent manner up to 5 ng/ml. Concentrations > 5 ng/ml showed a gradual decline in collagen synthesis. Basal collagen synthesis was increased in diabetic glomeruli, but addition of TGF-β 1 had no effect. Antibody to TGF-β1 prevented this increase in collagen synthesis. Both circulating TGF-β1 concentration and its glomerular expression of mRNA were higher in diabetic than in normal rats. Although addition of TGF-β1 did not increase synthesis in vitro, the absence of an effect is consistent with downregulation of its receptors attributable to the high circulating levels. This study clearly indicates a regulatory role of TGF-β1 in renal glomerular collagen synthesis in the normal rat, and suggests a possible causal role for enhanced collagen synthesis in the diabetic rat.
Skip Nav Destination
Article navigation
Original Articles|
November 01 1993
Transforming Growth Factor-β1 Enhances Glomerular Collagen Synthesis in Diabetic Rats Free
Jaya S Bollineni;
Jaya S Bollineni
Department of Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School
Newark, New Jersey
Search for other works by this author on:
Alluru S Reddi
Alluru S Reddi
Department of Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School
Newark, New Jersey
Search for other works by this author on:
Address correspondence and reprint requests to Dr. Alluru S. Reddi, Department of Medicine, UMDNJ-New Jersey Medicine School, 185 South Orange Avenue, Newark, NJ 07103.
Diabetes 1993;42(11):1673–1677
Article history
Received:
April 07 1993
Revision Received:
July 01 1993
Accepted:
July 01 1993
PubMed:
8405711
Citation
Jaya S Bollineni, Alluru S Reddi; Transforming Growth Factor-β1 Enhances Glomerular Collagen Synthesis in Diabetic Rats. Diabetes 1 November 1993; 42 (11): 1673–1677. https://doi.org/10.2337/diab.42.11.1673
Download citation file:
47
Views