The nonobese diabetic mouse in a model of spontaneous development of autoimmune type I diabetes. The disease can be induced in young, irradiated recipients by injecting splenic T-cells from diabetic donors. The adoptive transfer of diabetes requires the presence of both CD4+ and CD8+ splenic T-cell subsets. To test whether diabetogenic cells distribute in other lymphoid organs of diabetic mice, we first analyzed lymph node cells. Lymph node cells were much less efficient in transferring diabetes than splenocytes. This inefficacious transfer was not attributable to the absence of hematopoietic precursors or a lack of macrophages. Lymph node cells did not protect from the transfer of diabetes by splenocytes, indicating the absence of suppressor cells. Although CD8+ lymph node T-cells seemed functionally comparable to CD8+ splenocytes, CD4+ lymph node T-cells failed to cooperate with CD8+ splenocytes to transfer diabetes. Our study suggests that diabetogenic cells are not evenly distributed in the different lymphoid organs. This may reflect a differential migration pattern of pathogenic T-cells in this animal model.
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December 01 1993
Lymph Node T-Cells Do Not Optimally Transfer Diabetes in NOD Mice
Françoise Lepault;
Françoise Lepault
Hôpital Necker, CNRS URA 1461, Paris, and INSERM U25
Paris, France
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Christelle Faveeuw;
Christelle Faveeuw
Hôpital Necker, CNRS URA 1461, Paris, and INSERM U25
Paris, France
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Jian Jian Luan;
Jian Jian Luan
Hôpital Necker, CNRS URA 1461, Paris, and INSERM U25
Paris, France
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Marie-Claude Gagnerault
Marie-Claude Gagnerault
Hôpital Necker, CNRS URA 1461, Paris, and INSERM U25
Paris, France
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Address correspondence and reprint requests to Dr. F. Lepault, CNRS URA 1461, Hèpital Necker, 161 rue de Sevres, 75015 Paris, France.
Diabetes 1993;42(12):1823–1828
Article history
Received:
February 19 1993
Revision Received:
July 08 1993
Accepted:
July 08 1993
PubMed:
8243828
Citation
Françoise Lepault, Christelle Faveeuw, Jian Jian Luan, Marie-Claude Gagnerault; Lymph Node T-Cells Do Not Optimally Transfer Diabetes in NOD Mice. Diabetes 1 December 1993; 42 (12): 1823–1828. https://doi.org/10.2337/diab.42.12.1823
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