Evaluations of glomerular mRNA levels encoding for PCNA, TNF-α, PDGF-A and -B chains, TGF-β, IGF-I, bFGF, and EGF were made at 4, 12, and 24 wk after injection of STZ in Sprague-Dawley rats. The mRNA levels for PCNA, TNF-α, PDGF-B chain, TGF-β, and bFGF increased with age in STZ-induced diabetic rats. At 24 wk after STZ injection, mRNA levels for PCNA, TNF-α, PDGF-B chain, TGF-β, and bFGF were increased 3.8-fold, (P < 0.01), 4.2-fold (P < 0.01), 4.0-fold (P < 0.01), 5.2-fold (P < 0.001), and 3.6-fold (P < 0.01), respectively, in the glomeruli of diabetic rats when compared with control rats. In contrast, mRNA levels for IGF-I, PDGF-A chain, and EGF were not altered in glomeruli from diabetic and control rats throughout the experimental period. Insulin treatment partially ameliorated the increase in mRNA levels for PCNA, TNF-α, PDGF-B chain, TGF-β, and bFGF in the glomeruli of diabetic rats. These data indicate that alterations in growth factor mRNA levels in glomeruli may be a manifestation of diabetic nephropathy, and that hyperglycemia or insulin deficiency may play a role in abnormal growth factor gene regulation.
mRNA Expression of Growth Factors in Glomeruli From Diabetic Rats
Tsukasa Nakamura, Mitsumine Fukui, Isao Ebihara, Shiori Osada, Isao Nagaoka, Yasuhiko Tomino, Hikaru Koide; mRNA Expression of Growth Factors in Glomeruli From Diabetic Rats. Diabetes 1 March 1993; 42 (3): 450–456. https://doi.org/10.2337/diab.42.3.450
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