Evaluations of glomerular mRNA levels encoding for PCNA, TNF-α, PDGF-A and -B chains, TGF-β, IGF-I, bFGF, and EGF were made at 4, 12, and 24 wk after injection of STZ in Sprague-Dawley rats. The mRNA levels for PCNA, TNF-α, PDGF-B chain, TGF-β, and bFGF increased with age in STZ-induced diabetic rats. At 24 wk after STZ injection, mRNA levels for PCNA, TNF-α, PDGF-B chain, TGF-β, and bFGF were increased 3.8-fold, (P < 0.01), 4.2-fold (P < 0.01), 4.0-fold (P < 0.01), 5.2-fold (P < 0.001), and 3.6-fold (P < 0.01), respectively, in the glomeruli of diabetic rats when compared with control rats. In contrast, mRNA levels for IGF-I, PDGF-A chain, and EGF were not altered in glomeruli from diabetic and control rats throughout the experimental period. Insulin treatment partially ameliorated the increase in mRNA levels for PCNA, TNF-α, PDGF-B chain, TGF-β, and bFGF in the glomeruli of diabetic rats. These data indicate that alterations in growth factor mRNA levels in glomeruli may be a manifestation of diabetic nephropathy, and that hyperglycemia or insulin deficiency may play a role in abnormal growth factor gene regulation.
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Original Articles|
March 01 1993
mRNA Expression of Growth Factors in Glomeruli From Diabetic Rats
Tsukasa Nakamura;
Tsukasa Nakamura
Division of Nephrology, Department of Medicine and Biochemistry, Juntendo University School of Medicine
Tokyo, Japan
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Mitsumine Fukui;
Mitsumine Fukui
Division of Nephrology, Department of Medicine and Biochemistry, Juntendo University School of Medicine
Tokyo, Japan
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Isao Ebihara;
Isao Ebihara
Division of Nephrology, Department of Medicine and Biochemistry, Juntendo University School of Medicine
Tokyo, Japan
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Shiori Osada;
Shiori Osada
Division of Nephrology, Department of Medicine and Biochemistry, Juntendo University School of Medicine
Tokyo, Japan
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Isao Nagaoka;
Isao Nagaoka
Division of Nephrology, Department of Medicine and Biochemistry, Juntendo University School of Medicine
Tokyo, Japan
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Yasuhiko Tomino;
Yasuhiko Tomino
Division of Nephrology, Department of Medicine and Biochemistry, Juntendo University School of Medicine
Tokyo, Japan
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Hikaru Koide
Hikaru Koide
Division of Nephrology, Department of Medicine and Biochemistry, Juntendo University School of Medicine
Tokyo, Japan
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Address correspondence and reprint requests to Hikaru Koide, MD, Division of Nephrology, Department of Medicine, Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo 113, Japan.
Diabetes 1993;42(3):450–456
Article history
Received:
June 08 1992
Revision Received:
November 05 1992
Accepted:
November 05 1992
PubMed:
8094359
Citation
Tsukasa Nakamura, Mitsumine Fukui, Isao Ebihara, Shiori Osada, Isao Nagaoka, Yasuhiko Tomino, Hikaru Koide; mRNA Expression of Growth Factors in Glomeruli From Diabetic Rats. Diabetes 1 March 1993; 42 (3): 450–456. https://doi.org/10.2337/diab.42.3.450
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