Glucose metabolism and respiratory burst were studied in vitro in resident peritoneal macrophages from non-diabetes-prone BB, spontaneously diabetic BB, diabetes-prone BB, and STZ-induced diabetic BBn rats, in the presence or absence of phorbol myristate acetate plus ionomycin. Glycolysis and pentose phosphate pathway activity were increased in BBd compared with BBn cells. PMA plus IONO did not influence glycolysis in BBn macrophages and slightly decreased it in BBd macrophages. In contrast, PMA plus IONO increased the pentose phosphate pathway activity in BBn and BBd macrophages with a much greater increase in BBd cells. The release of O2− was greater in BBd than BBn cells; PMA plus IONO also induced a much greater release of O2− in BBd cells. H2O2 release was undetectable in unstimulated BBn cells, and stimulation by PMA plus IONO caused a small incremental release. In contrast, the release of H2O2 was measurable in unstimulated cells and further increased by 50% in BBd cells with PMA-plus-IONO stimulation. The release of O2− and H2O2 was increased in macrophages from 75-day-old BBdp rats but not in 50-day-old BBdp rats, compared with age-matched BBn rats. No differences were observed in either glucose metabolism or release of O2− and H2O2 between BBn and STZ-BBn cells in the absence or presence of PMA plus IONO. These data suggest that enhanced oxidative metabolism in BBd macrophages is unlikely to be attributable to diabetes per se. Furthermore, the results are consistent with prior in vivo immunological activation of BBd macrophages. Increased production of 02− and H2O2 from activated macrophages may be involved in the autoimmune destruction of β-cells that causes the diabetic syndrome.
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Original Articles|
April 01 1993
Enhanced Glucose Metabolism and Respiratory Burst in Peritoneal Macrophages From Spontaneously Diabetic BB Rats
Guoyao Wu;
Guoyao Wu
McGill Nutrition and Food Science Centre, Royal Victoria Hospital
Montreal, Canada
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Errol B Marliss
Errol B Marliss
McGill Nutrition and Food Science Centre, Royal Victoria Hospital
Montreal, Canada
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Address correspondence and reprint requests to Errol B. Marliss, MD, McGill Nutrition and Food Science Center, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec H3A 1A1, Canada.
Diabetes 1993;42(4):520–529
Article history
Received:
January 10 1992
Revision Received:
November 30 1992
Accepted:
November 30 1992
PubMed:
8384132
Citation
Guoyao Wu, Errol B Marliss; Enhanced Glucose Metabolism and Respiratory Burst in Peritoneal Macrophages From Spontaneously Diabetic BB Rats. Diabetes 1 April 1993; 42 (4): 520–529. https://doi.org/10.2337/diab.42.4.520
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