We have shown previously that thoracic duct lymph insulin dynamics are well correlated with tracer-determined whole-body glucose uptake and have suggested that thoracic duct lymph insulin is representative of insulin concentration in muscle interstitial fluid. However, thoracic duct lymph is comprised of interstitial fluid from all sub-thoracic tissue beds. To investigate the relative contribution of muscle interstitial fluid to total thoracic duct lymph flow, the distribution and elimination of [14C]inulin was investigated in eight experiments with conscious dogs. Both plasma and thoracic duct lymph were measured, and a three-compartment model that was hypothesized to consist of plasma, splanchnic interstitial fluid, and muscle interstitial fluid was identified. Identifications were performed with either a bolus protocol (n = 4) or an infusion protocol (n = 4), and the predicted [14C]inulin dynamics in the splanchnic and muscle interstitial fluid compartments were compared with measured values in thoracic duct lymph. Neither compartment predicted the thoracic duct [14C]inulin dynamics; however, a model based on a percentage contribution from each tissue bed fit the thoracic data well. The relative contribution of splanchnic interstitial fluid to the total thoracic duct lymph flow averaged 78 ± 5% for the bolus protocol and 54 ± 5% for the infusion protocol. Thus, we conclude that in the conscious animal, ∼25–50% of thoracic duct lymph originates from muscle interstitial fluid.
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May 01 1993
Thoracic Duct Lymph: Relative Contribution From Splanchnic and Muscle Tissue
Garry M Steil;
Garry M Steil
Department of Physiology and Biophysics, University of Southern California Medical School
Los Angeles, California
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Melissa A Meador;
Melissa A Meador
Department of Physiology and Biophysics, University of Southern California Medical School
Los Angeles, California
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Richard N Bergman
Richard N Bergman
Department of Physiology and Biophysics, University of Southern California Medical School
Los Angeles, California
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Address correspondence and reprint requests to Richard N. Bergman, PhD, Department of Physiology and Biophysics, USC Medical School, 1333 San Pablo, Los Angeles, CA 90033.
Diabetes 1993;42(5):720–731
Article history
Received:
April 14 1992
Revision Received:
December 23 1992
Accepted:
December 23 1992
PubMed:
8482429
Citation
Garry M Steil, Melissa A Meador, Richard N Bergman; Thoracic Duct Lymph: Relative Contribution From Splanchnic and Muscle Tissue. Diabetes 1 May 1993; 42 (5): 720–731. https://doi.org/10.2337/diab.42.5.720
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