Pancreatic βTC lines derived from insulinomas arising in transgenic mice expressing SV40 Tag under control of the insulin promoter manifest a differentiated β-cell phenotype and secrete insulin in response to glucose. Previously reported βTC lines respond to subphysiological extracellular glucose levels compared with normal β-cells. Recently, several βTC lines were developed with normal glucose-regulated insulin secretion from insulinomas obtained by breeding of the RIP-Tag transgene from the original C57BI/6 mouse strain into the C3HeB/FeJ strain. One of these βTC lines, βTC7, was characterized in detail. βTC7 cells express GLUT2 and have levels of glucokinase and hexokinase activity similar to those of normal islets. As a result these cells exhibit a normal glucose concentration dependency for glycolysis and insulin secretion, thus representing an accurate model of β-cell function. On continuous propagation in culture, βTC7 cells acquired a response to lower extracellular glucose levels. This change was associated with a fourfold increase in hexokinase activity, without significant changes in glucokinase activity and glucose uptake rates. These findings suggest an important role for glucose phosphorylation rates in regulation of the β-cell insulin secretory response to glucose.
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Original Articles|
June 01 1993
Murine Insulinoma Cell Line With Normal Glucose-Regulated Insulin Secretion
Shimon Efrat;
Shimon Efrat
Departments of Molecular Pharmacology and Medicine and the Diabetes Research and Training Center, Albert Einstein College of Medicine
Bronx, New York
Department of Cell Biology, Weizmann Institute of Science
Rehovot, Israel
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Margarit Leiser;
Margarit Leiser
Departments of Molecular Pharmacology and Medicine and the Diabetes Research and Training Center, Albert Einstein College of Medicine
Bronx, New York
Department of Cell Biology, Weizmann Institute of Science
Rehovot, Israel
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Manju Surana;
Manju Surana
Departments of Molecular Pharmacology and Medicine and the Diabetes Research and Training Center, Albert Einstein College of Medicine
Bronx, New York
Department of Cell Biology, Weizmann Institute of Science
Rehovot, Israel
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Michael Tal;
Michael Tal
Departments of Molecular Pharmacology and Medicine and the Diabetes Research and Training Center, Albert Einstein College of Medicine
Bronx, New York
Department of Cell Biology, Weizmann Institute of Science
Rehovot, Israel
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David Fusco-Demane;
David Fusco-Demane
Departments of Molecular Pharmacology and Medicine and the Diabetes Research and Training Center, Albert Einstein College of Medicine
Bronx, New York
Department of Cell Biology, Weizmann Institute of Science
Rehovot, Israel
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Norman Fleischer
Norman Fleischer
Departments of Molecular Pharmacology and Medicine and the Diabetes Research and Training Center, Albert Einstein College of Medicine
Bronx, New York
Department of Cell Biology, Weizmann Institute of Science
Rehovot, Israel
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Address correspondence and reprint requests to Norman Fleischer, Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461.
Diabetes 1993;42(6):901–907
Article history
Received:
July 29 1992
Revision Received:
January 21 1993
Accepted:
January 21 1993
Citation
Shimon Efrat, Margarit Leiser, Manju Surana, Michael Tal, David Fusco-Demane, Norman Fleischer; Murine Insulinoma Cell Line With Normal Glucose-Regulated Insulin Secretion. Diabetes 1 June 1993; 42 (6): 901–907. https://doi.org/10.2337/diab.42.6.901
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