Glucagon-like peptide-1 is a fragment of proglucagon secreted by intestinal L-cells. It has potent glucose-dependent insulin secretory effects and also suppresses gastric acid secretion in the stomach. The biological actions of GLP-1 are mediated by the GLP-1 receptor, the structure of which has recently been determined. Defects in insulin secretion are a common feature of NIDDM and as such the GLP-1 receptor is a candidate for contributing to the development of this clinically and genetically heterogeneous disorder. As a first step in determining the role of the GLP-1 receptor in the development of NIDDM, we have isolated the human GLP-1 receptor gene and mapped it to chromosome 6, band p21.1, using the technique of fluorescence in situ hybridization. We also identified a simple tandem repeat DNA polymorphism in the human GLP-1 receptor gene of the form (TG)n. This DNA polymorphism has 14 alleles and a heterozygosity of >0.8. We have used this DNA polymorphism to localize the GLP-1 receptor gene within the genetic map of the short arm of chromosome 6. This DNA polymorphism will facilitate genetic studies of the contribution of the GLP-1 receptor gene to impaired β-cell function and NIDDM.
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August 01 1993
Human Glucagon-Like Peptide-1 Receptor Gene: Localization to Chromosome Band 6p21 by Fluorescence In Situ Hybridization and Linkage of a Highly Polymorphic Simple Tandem Repeat DNA Polymorphism to Other Markers on Chromosome 6
Markus Stoffel;
Markus Stoffel
Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biology, and Medicine, The University of Chicago
Chicago, Illinois
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Rafael Espinosa, III;
Rafael Espinosa, III
Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biology, and Medicine, The University of Chicago
Chicago, Illinois
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M Le Beau Michelle;
M Le Beau Michelle
Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biology, and Medicine, The University of Chicago
Chicago, Illinois
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Graeme I Bell
Graeme I Bell
Howard Hughes Medical Institute and Departments of Biochemistry and Molecular Biology, and Medicine, The University of Chicago
Chicago, Illinois
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Address correspondence and reprint requests to Dr. Graeme I. Bell, Howard Hughes Medical Institute, 5841 South Maryland Avenue, MC1028, Chicago, IL 60637.
Diabetes 1993;42(8):1215–1218
Article history
Received:
April 08 1993
Revision Received:
April 22 1993
Accepted:
April 22 1993
Citation
Markus Stoffel, Rafael Espinosa, M Le Beau Michelle, Graeme I Bell; Human Glucagon-Like Peptide-1 Receptor Gene: Localization to Chromosome Band 6p21 by Fluorescence In Situ Hybridization and Linkage of a Highly Polymorphic Simple Tandem Repeat DNA Polymorphism to Other Markers on Chromosome 6. Diabetes 1 August 1993; 42 (8): 1215–1218. https://doi.org/10.2337/diab.42.8.1215
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