Anesthetized male Sprague-Dawley rats (350–400 g) were injected intravenously with either 0.1, 1, 15, or 25 nmol rat islet amyloid polypeptide (IAPP), 65 or 650 pmol rat calcitonin gene-related peptide (CGRP), or saline alone. IAPP at the two highest doses decreased the mean arterial blood pressure (BP), increased blood glucose concentrations, and decreased serum insulin concentrations. CGRP at both doses decreased the BP but did not affect the blood glucose concentrations. The blood flow to the whole pancreas, pancreatic islets, adrenal glands, colon, duodenum, liver, and kidney was measured with a microsphere technique 30 min after administration of IAPP and 3 min after injection of CGRP. The two higher doses of IAPP (15 and 25 nmol) markedly reduced the whole pancreatic blood flow, whereas the islet blood flow remained unaffected. This resulted in an increase in the fraction of whole pancreatic blood flow diverted through the islets from ∼ 10 to 17%. No blood flow changes in the pancreas or the islets were observed when 0.1 or 1 nmol IAPP was injected. CGRP at both doses caused a decrease in both whole pancreatic and islet blood flow. No changes in fractional islet blood flow were observed, despite similar effects on mean arterial BP as observed after IAPP injections. Neither adrenal, duodenal, colonic, hepatic, skeletal muscle, nor renal blood flow were significantly affected by any of the concentrations of IAPP used, whereas 650 pmol CGRP decreased both duodenal and colonic blood flow. We conclude that IAPP and CGRP have different effects on pancreatic islet blood flow and that IAPP may be of importance for islet blood flow regulation.
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Original Articles|
March 01 1994
Pancreatic Islet Blood Flow in the Rat After Administration of Islet Amyloid Polypeptide or Calcitonin Gene-Related Peptide
Annika M Svensson;
Annika M Svensson
Department of Medical Cell Biology, Uppsala University
Uppsala, Sweden
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Stellan Sandler;
Stellan Sandler
Department of Medical Cell Biology, Uppsala University
Uppsala, Sweden
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Leif Jansson
Leif Jansson
Department of Medical Cell Biology, Uppsala University
Uppsala, Sweden
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Address correspondence and reprint requests to Dr. Annika M. Svensson, Department of Medical Cell Biology, Biomedical Center, P.O. Box 571, S-751 23, Uppsala, Sweden.
Diabetes 1994;43(3):454–458
Article history
Received:
March 30 1993
Revision Received:
October 07 1993
Accepted:
October 07 1993
PubMed:
8314019
Citation
Annika M Svensson, Stellan Sandler, Leif Jansson; Pancreatic Islet Blood Flow in the Rat After Administration of Islet Amyloid Polypeptide or Calcitonin Gene-Related Peptide. Diabetes 1 March 1994; 43 (3): 454–458. https://doi.org/10.2337/diab.43.3.454
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