Because of anatomical limitations, molecular characterization of islet-inflammatory T-cells in human insulin-dependent diabetes mellitus (IDDM) has remained elusive. We have isolated isletitis T-cells from pancreas graft biopsies of two patients (syngeneic and allogeneic, respectively) shortly after onset of recurrent IDDM and have characterized their repertoire by sequencing T-cell receptor (TcR)-specific cDNAs. Histopathological analysis of the grafts revealed selective β-cell loss and isletitis characteristic of recurrent disease with no evidence of chronic inflammation or rejection. Most of the in vivo-activated isletitis T-cells were CD8+TcRαβ+ and CD4−CD8−TcRγδ+ in both patients. Comparison of the different TcRα,β,γ, and δ sequences revealed V(D)J junctional heterogeneity but skewed TcR usage within patients. Eighth of 13 different isletitis TcRβ sequences (19 of 26 cDNAs) from the syngeneic graft of patient 1 were Vβ3+, as opposed to only 1 of 31 peripheral TcRβ sequences (1 of 31 cDNAs) (61.5 vs. 3.2%, P < 0.0001). Of the 19 different isletitis TcRα clonotypes of this patient (24 of 42 cDNAs), 5 were Vα14+. The isletitis TcRβ clonotypes of the human leukocyte antigen-identical allogeneic graft of patient 2 showed selective Jβ, but not Vβ, gene usage. Two of three predominant isletitis clonotypes of patient 2 were Vα22+ (19 of 28 cDNAs) and the other (5 of 28 cDNAs) was also Vα14+. As opposed to peripherl γδ T-cell, which usedall Vγ and Vδ gene families, isletitis γδ T-cells only used VγI, Vγ2, Vδ1, and Vδ2 (patient 1) or VγI, Vγ2, Vγ3, and Vδ1 (patient 2) genes. These data are compatible with preferential recruitment of certain CD8+ and CD4−CD8− T-cells to islets on the basis of TcR-V and/or J gene usage, which varies among patients, but favors a response against multiple target major histocompatibility complex/peptide complexes.
Skewed T-Cell Receptor Usage and Junctional Heterogeneity Among Isletitis αβ and γδ T-cells in Human IDDM
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Pere Santamaria, Cinthia Lewis, Jose Jessurun, David E R Sutherland, Jose J Barbosa; Skewed T-Cell Receptor Usage and Junctional Heterogeneity Among Isletitis αβ and γδ T-cells in Human IDDM. Diabetes 1 April 1994; 43 (4): 599–606. https://doi.org/10.2337/diab.43.4.599
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