A fraction from normal human plasma inhibiting Na+ -K+ ATPase has been recently identified as lysophosphatidylcholine (LPC). The aim of this study was to investigate the existence of a relationship between the activity of the cellular membrane Na+ -K− -ATPase and plasma LPC in human diabetes. We studied 10 patients with insulin-dependent-diabetes mellitus (IDDM), 14 patients with non-insulin-dependent diabetes mellitus (NIDDM), and 10 sex- and age-matched control subjects. Plasma LPC concentrations were increased in both IDDM and NIDDM patients compared with control subjects. Na+ -K+ -ATPase activity was reduced in both groups of patients in erythrocyte and platelet membranes. There was a significant correlation between the concentrations of plasma LPC and Na+ -K+ -ATPase activity in both erythrocyte and platelet membranes (P < 0.01). To investigate the effect of LPC on the enzyme, Na+ -K+ -ATPase activity was determined in erythrocyte membranes obtained from six healthy subjects after in vitro incubation with increasing concentrations of LPC (1–10 microM). Enzymatic activity was significantly reduced by in vitro LPC at a concentration of 2.5 microM, with a further decrease at 5 microM. These data suggest that the decrease in Na+ -K+ -ATPase activity in diabetes might be due to increased LPC concentrations.
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Original Articles|
July 01 1994
Reduced Na+ -K+ -ATPase Activity and Plasma Lysophosphatidylcholine Concentrations in Diabetic Patients
Rosa A Rabini;
Rosa A Rabini
Department of Diabetology, INRCA Hospital
Ancona, Italy
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Roberto Galassi;
Roberto Galassi
Department of Diabetology, INRCA Hospital
Ancona, Italy
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Paolo Fumelli;
Paolo Fumelli
Department of Diabetology, INRCA Hospital
Ancona, Italy
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Nicole Dousset;
Nicole Dousset
Department of Biochemistry, CHU Rangueil
Toulouse, France
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Marie L Solera;
Marie L Solera
Department of Biochemistry, CHU Rangueil
Toulouse, France
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Pierre Valdiguie;
Pierre Valdiguie
Department of Biochemistry, CHU Rangueil
Toulouse, France
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Giovanna Curatola;
Giovanna Curatola
Institute of Biochemistry, University of Ancona
Ancona, Italy
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Gianna Ferretti;
Gianna Ferretti
Institute of Biochemistry, University of Ancona
Ancona, Italy
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Marina Taus;
Marina Taus
Institute of Biochemistry, University of Ancona
Ancona, Italy
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Laura Mazzanti
Laura Mazzanti
Institute of Biochemistry, University of Ancona
Ancona, Italy
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Address correspondence and reprint requests to Dr. Rosa Anna Rabini, Reparto di Diabetologia, Ospedale Geriatrico, via della Montagnola 164, 60125 Ancona, Italy.
Diabetes 1994;43(7):915–919
Article history
Received:
June 11 1993
Revision Received:
March 17 1994
Accepted:
March 17 1994
PubMed:
8013757
Citation
Rosa A Rabini, Roberto Galassi, Paolo Fumelli, Nicole Dousset, Marie L Solera, Pierre Valdiguie, Giovanna Curatola, Gianna Ferretti, Marina Taus, Laura Mazzanti; Reduced Na+ -K+ -ATPase Activity and Plasma Lysophosphatidylcholine Concentrations in Diabetic Patients. Diabetes 1 July 1994; 43 (7): 915–919. https://doi.org/10.2337/diab.43.7.915
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