Nitric oxide (NO) is believed to be an effector molecule that mediates interleuMn (IL)-1β-induced destruction and dysfunction of pancreatic β-cells. We have demonstrated that both exogenous NO and NO generated endogenously by IL-1β brought about apoptosis of isolated rat pancreatic islet cells as well as pancreatic β-cell tumorderived cell line HIT. This apoptosis was characterized by cleavage of DNA into nucleosomal fragments of 180–200 bp and morphologically by nuclear shrinkage, chromatic condensation, and apoptotic body formation. The EL-1β-induced internucleosomal DNA cleavage occurred in a time- and dose-dependent manner. Actinomycin D, cycloheximide, and nitric oxide synthase inhibitors inhibited the DNA cleavage, which was correlated with the amount of NO produced, indicating that NO produced by HIT cells themselves could mediate the apoptosis. Furthermore, in the presence of tumor necrosis factor (TNF)-α, large amounts of NO were produced by IL-1β and DNA cleavage occurred more noticeably, although TNF-β alone did not generate NO. Streptozotocin (STZ), a diabetogenic reagent containing a nitroso moiety, also released NO and induced internucleosomal DNA cleavage in HIT cells. These results suggest that NO-induced internucleosomal DNA cleavage is an important initial step in the destruction and dysfunction of pancreatic (β-cells induced by inflammatory stimulation or treatment with STZ.
Apoptotic Cell Death Triggered by Nitric Oxide in Pancreatic β-Cells
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Hideaki Kaneto, Junichi Fujii, Han Geuk Seo, Keiichiro Suzuki, Taka-aki Matsuko, Nakamura Masahiro, Haruyuki Tatsumi, Yoshimitsu Yamasaki, Takenobu Kamada, Naoyuki Taniguchi; Apoptotic Cell Death Triggered by Nitric Oxide in Pancreatic β-Cells. Diabetes 1 July 1995; 44 (7): 733–738. https://doi.org/10.2337/diab.44.7.733
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