The purpose of the study was to evaluate fasting endothelin-1 levels in subjects with syndrome X, in subjects with insulinoma, and in normal subjects. The single and synergistic contributions of insulin and triglyceride levels to endothelin-1 release were studied in normal subjects. This was achieved by the evaluation of endothelin-1 levels in response to an insulin bolus combined with a euglycemic clamp (protocol A) and during intralipid (test 1) or saline (test 2) infusions lasting 360 min (protocol B). In protocol B, a euglycemic two-step hyperinsulinemic (25 and 125 mU · kg−1 · h−1) clamp was started at 120 min. Subjects with syndrome × showed significantly higher endothelin-1 levels than normal subjects and subjects with insulinoma (7.22 ± 0.89 vs. 2.61 ± 0.38 and 2.49 ± 0.24 pg/ml, P < 0.01). After an insulin bolus, endothelin-1 levels peaked at 10 min (3.71 ± 0.96 pg/ml). The incremental area of endothelin-1 was significantly higher after insulin than after a saline bolus. In test 1, an acute increase in triglyceride levels significantly enhanced endothelin-1 levels, with were further increased by the synergistic contribution of high insulin and triglyceride levels. In test 2, endothelin-1 release was achieved at high insulin levels but remained significantly lower than in test 1. In conclusion, subjects with syndrome × showed higher endothelin-1 levels than normal subjects and subjects with insulinoma. These levels were reproduced in normal subjects by a simultaneous increase in insulin and triglyceride levels.
Skip Nav Destination
Article navigation
Original Articles|
March 01 1996
Hypertriglyceridemia and Hyperinsulinemia Are Potent Inducers of Endothelin-1 Release in Humans
PierMarco Piatti;
PierMarco Piatti
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Lycilla D Monti;
Lycilla D Monti
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Morena Conti;
Morena Conti
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Laura Baruffaldi;
Laura Baruffaldi
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Laura Galli;
Laura Galli
Dipartimento di Statistica Medica ed Epidemiologia University of Milan
Milan, Italy
Search for other works by this author on:
Chuong V Phan;
Chuong V Phan
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Barbara Guazzini;
Barbara Guazzini
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Antonio E Pontiroli;
Antonio E Pontiroli
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Guido Pozza
Guido Pozza
Istituto Scientiflco H. San Raffaele, Cattedra di Clinica Medica
Italy
Search for other works by this author on:
Address correspondence and reprint requests to Dr. PierMarco Piatti, Istituto Scientiiico H San Raffaele, Via Olgettina 60, 20132 Milan, Italy.
Diabetes 1996;45(3):316–321
Article history
Received:
May 24 1995
Revision Received:
October 30 1995
Accepted:
October 30 1995
PubMed:
8593936
Citation
PierMarco Piatti, Lycilla D Monti, Morena Conti, Laura Baruffaldi, Laura Galli, Chuong V Phan, Barbara Guazzini, Antonio E Pontiroli, Guido Pozza; Hypertriglyceridemia and Hyperinsulinemia Are Potent Inducers of Endothelin-1 Release in Humans. Diabetes 1 March 1996; 45 (3): 316–321. https://doi.org/10.2337/diab.45.3.316
Download citation file:
69
Views