Recently, a trinucleotide repeat polymorphism at the rad (ras associated with diabetes) locus (RAD1) on chromosome 16q was described in association with NIDDM in white Americans. In an attempt to replicate this finding, we screened RAD1 and another microsatellite marker at the D16S265 loci, which is located near the rad locus, with a radioactive polymerase chain reaction method in 290 unrelated Finnish NIDDM patients and 270 control subjects and related the findings to measures of insulin sensitivity. Both groups were randomly selected from the western (189 NIDDM patients and 184 control subjects) and southern (101 NIDDM and 86 control subjects) parts of Finland. The allele frequency distributions of RAD1 and D16S265 did not differ between NIDDM patients and control subjects in the studied population groups. The genotype distribution was also analyzed by structural classes of the RAD1 polymorphism, and no difference was detected between the NIDDM and control groups. In addition, carriers of allele classes I, II, and IV (reported to be preferentially associated with NIDDM in white Americans) did not differ from the class III homozygotes with respect to age at onset of NIDDM, BMI, or rates of insulin-stimulated glucose disposal. In conclusion, we found no association between the rad locus and NIDDM or insulin resistance in Finnish NIDDM patients.
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Original Articles|
April 01 1996
Polymorphism at the rad Gene Is Not Associated With NIDDM in Finns
Marju Orho;
Marju Orho
Diabetes and Endocrine Research Unit, Wallenberg Laboratory, University of Lund
Sweden
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Martin Carlsson;
Martin Carlsson
Diabetes and Endocrine Research Unit, Wallenberg Laboratory, University of Lund
Sweden
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Timo Kanninen;
Timo Kanninen
Diabetes and Endocrine Research Unit, Wallenberg Laboratory, University of Lund
Sweden
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Leif C Groop
Leif C Groop
Diabetes and Endocrine Research Unit, Wallenberg Laboratory, University of Lund
Sweden
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Address correspondence and reprint requests to Prof. Leif C. Groop, Department of Endocrinology, University Hospital, MAS, Entrance 51B, 205 02 Malmo, Sweden.
Diabetes 1996;45(4):429–433
Article history
Received:
July 13 1995
Revision Received:
November 16 1995
Accepted:
November 16 1995
PubMed:
8603763
Citation
Marju Orho, Martin Carlsson, Timo Kanninen, Leif C Groop; Polymorphism at the rad Gene Is Not Associated With NIDDM in Finns. Diabetes 1 April 1996; 45 (4): 429–433. https://doi.org/10.2337/diab.45.4.429
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