Increased susceptibility of LDL to oxidation has been shown to be associated with the presence of coronary heart disease and may account for the accelerated vascular disease seen in diabetes. The response of LDL to in vitro oxidative stress has been proposed as a measure of the predisposition of LDL to the in vivo subendothelial oxidative stress. Increased susceptibility to oxidation has been demonstrated recently in diabetic patients with poorly controlled IDDM. Thus, we conducted studies to determine whether the increased susceptibility of LDL to oxidation was secondary to diabetes per se or to the level of glycemic control. Fifteen IDDM patients with good glycemic control and with no evidence of macrovascular disease or proteinuria were compared with healthy age-, sex-, race-, and BMI-matched nondiabetic subjects. Fasting blood glucose levels averaged 12.1 ± 1.1 (mean ± SE) vs. 4.9 ± 0.1 mmol/l in the diabetic versus the control groups, respectively. HbAlc levels averaged 7.7 ± 0.5 vs. 4.4 ± 0.2%, reflecting well-controlled diabetes (P < 0.0001). Total, LDL, VLDL, and HDL cholesterol, triglyceride, and lipoprotein(a) levels did not differ between the groups. The particle size, lipid composition, fatty acid content, antioxidant content, and glycation were similar for LDL isolated from both groups. A rapid LDL preparation technique was used to compare LDL susceptibility to oxidation under the following conditions: final LDL cholesterol concentration of 100 μg/ml, 5 μmol/l of CuCl2 at 25°C. There was no difference in the susceptibility to in vitro oxidation of LDL isolated from IDDM patients compared with control subjects. There was no correlation of glycemic control with any of the parameters of the in vitro oxidation of LDL. LDL from patients with well-controlled IDDM does not differ in composition or in susceptibility to in vitro oxidative stress compared with LDL from nondiabetic subjects.
Skip Nav Destination
Article navigation
Original Articles|
June 01 1996
LDL From Patients With Well-Controlled IDDM Is Not More Susceptible to In Vitro Oxidation
Alicia J Jenkins;
Alicia J Jenkins
Ralph H. Johnson Department of Veterans Affairs Medical Center
Charleston, South Carolina
Search for other works by this author on:
Richard L Klein;
Richard L Klein
Ralph H. Johnson Department of Veterans Affairs Medical Center
Charleston, South Carolina
Search for other works by this author on:
Charlyne N Chassereau;
Charlyne N Chassereau
Ralph H. Johnson Department of Veterans Affairs Medical Center
Charleston, South Carolina
Search for other works by this author on:
Kathie L Hermayer;
Kathie L Hermayer
Ralph H. Johnson Department of Veterans Affairs Medical Center
Charleston, South Carolina
Search for other works by this author on:
Maria F Lopes-Virella
Maria F Lopes-Virella
Ralph H. Johnson Department of Veterans Affairs Medical Center
Charleston, South Carolina
Search for other works by this author on:
Address correspondence and reprint requests to Dr. Maria F. Lopes-Virella, Research Service (151-5), Ralph H. Johnson Department of Veterans Affairs Medical Center, 109 Bee St., Charleston, SC 29401-5799.
Diabetes 1996;45(6):762–767
Article history
Received:
September 25 1995
Revision Received:
January 11 1996
Accepted:
January 11 1996
PubMed:
8635650
Citation
Alicia J Jenkins, Richard L Klein, Charlyne N Chassereau, Kathie L Hermayer, Maria F Lopes-Virella; LDL From Patients With Well-Controlled IDDM Is Not More Susceptible to In Vitro Oxidation. Diabetes 1 June 1996; 45 (6): 762–767. https://doi.org/10.2337/diab.45.6.762
Download citation file:
32
Views