Because the activity of mitochondrial glycerol phosphate dehydrogenase (mGPD) in the pancreatic β-cell is normally among the highest of the body, but is low in the pancreatic islets of numerous rodent models of NIDDM and is reported to be low in the islets of the few humans studied with NIDDM (1,2), it has been questioned whether mGPD might be a diabetes candidate gene. Linkage studies have failed to find an association between mGPD and NIDDM in Caucasians (3) and Mexican Americans (4), which suggests that mutations in mGPD are not a common cause of NIDDM. Since NIDDM is a multifactorial and heterogeneous disorder, it is possible that abnormalities in many individual genes influencing reactions that are important for the maintenance of normal glucose homeostasis in the β-cell will be found in a low percentage of individuals with NIDDM. With this idea in mind, conditions for using single-stranded conformational polymorphism (SSCP) analysis of the mGPD gene were developed to use this method to screen for mutations in this gene.
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Brief Genetics Reports|
October 01 1997
Single-Stranded Conformational Polymorphism Analysis of the Mitochondria! Glycerol Phosphate Dehydrogenase Gene in NIDDM
Michael J MacDonald;
Michael J MacDonald
University of Wisconsin Children's Diabetes Center
Madison, Wisconsin
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Laura J Brown;
Laura J Brown
University of Wisconsin Children's Diabetes Center
Madison, Wisconsin
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Noaman M Hasan;
Noaman M Hasan
University of Wisconsin Children's Diabetes Center
Madison, Wisconsin
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Markus Stoffel;
Markus Stoffel
Laboratory of Metabolic Diseases, Rockefeller University
New York, New York
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Diana G Dills
Diana G Dills
Division of Endocrinology, University of Wisconsin Medical School
Madison, Wisconsin
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Address correspondence and reprint requests to Dr. Michael J. MacDonald, Medical Science Center, Rm. 3459, 1300 University Ave., Madison, WI 53706.
Diabetes 1997;46(10):1660–1661
Article history
Received:
October 15 1996
Revision Received:
June 13 1997
Accepted:
June 13 1997
PubMed:
9313767
Citation
Michael J MacDonald, Laura J Brown, Noaman M Hasan, Markus Stoffel, Diana G Dills; Single-Stranded Conformational Polymorphism Analysis of the Mitochondria! Glycerol Phosphate Dehydrogenase Gene in NIDDM. Diabetes 1 October 1997; 46 (10): 1660–1661. https://doi.org/10.2337/diacare.46.10.1660
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