New cellular-based reagents are needed to diagnose type I diabetes as well as to monitor the outcomes of clinical trials at early time points. Four new monoclonal antibodies (mAbs) have been shown to demonstrate reduced binding to lymphocytes from identical twins with long-term type I diabetes relative to that observed with lymphocytes from their twin partners without diabetes or from control subjects. Biochemical analysis revealed mAb 3G12EG recognized an unidentified 45-kDa protein, whereas mAb 2E8F1 and 5B6E11 did not appear to precipitate specific proteins as detected by SDS-PAGE. Electrophoresis under reducing and nonreducing conditions and peptide mapping revealed that mAb 8F410 recognizes a novel dimeric form of HLA class I molecule. Predictions from crystallography studies suggested previously this class I dimer as the optimal activation of a single CD8 T-cell. In B-cells from both normal and diabetic individuals, the class I dimer was minimally associated with β2-microglobulin rapidly formed in the endoplasmic reticulum. These new reagents appear to be able to identify new lymphocyte surface phenotypes associated with diabetes expression in both fresh blood samples and Epstein-Barr virus–established cell lines.
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Original Articles|
March 01 1997
New Monoclonal Antibody Diagnostic Reagents for Type I Diabetes: Differential Lymphocyte Surface Antigen Expression Related to Disease
Jose A Pinies;
Jose A Pinies
Endocrinology Department, Cruces Hospital Baracaldo
Vizcaya, Basque Country, Spain
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Yineng Fu;
Yineng Fu
Immunobiology Laboratory, Massachusetts General Hospital
Boston, Massachusetts
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J A Vazquez;
J A Vazquez
Endocrinology Department, Cruces Hospital Baracaldo
Vizcaya, Basque Country, Spain
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Denise Faustman
Denise Faustman
Immunobiology Laboratory, Massachusetts General Hospital
Boston, Massachusetts
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Address correspondence and reprint requests to Dr. Denise Faustman, Massachusetts General Hospital-East, Immunobiology Laboratory, Building 149, 13th St., CNY-3601, Charlestown, MA 02129.
1
APC, antigen-presenting cell; EBV, Epstein-Barr virus; HBSS, Hanks' balanced salt solution; IgG, immunoglobulin G; mAb, monoclonal antibody; MAD, mean antigen density; MFI, mean fluorescence intensity; MHC, major histocompatibility complex; PBL, peripheral blood lymphocyte.
Diabetes 1997;46(3):363–371
Article history
Received:
May 20 1996
Revision Received:
October 29 1996
Accepted:
October 29 1996
PubMed:
9032090
Citation
Jose A Pinies, Yineng Fu, J A Vazquez, Denise Faustman; New Monoclonal Antibody Diagnostic Reagents for Type I Diabetes: Differential Lymphocyte Surface Antigen Expression Related to Disease. Diabetes 1 March 1997; 46 (3): 363–371. https://doi.org/10.2337/diab.46.3.363
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