To determine the relationship between mitochondrial DNA (mtDNA) mutations and age-related impaired glucose tolerance (IGT), mtDNA from skeletal muscle of 19 volunteers, ages 55–75 years, with either IGT or diabetes and 17 age- and sex-matched control subjects was analyzed using a long-extension polymerase chain reaction (PCR) combined with a quantitative PCR. We found the common 4,977-bp deletion in 84% of the IGT/diabetes group compared with only 41% in the control group (P < 0.02). Multiple other deletions of different sizes were identified in 13 out of 19 IGT/diabetes patients (68%) compared with 2 out of 17 control subjects (12%) (P < 0.002). Because of the heterogeneity and variation in the mutations identified, we propose that these mtDNA mutations were the result rather than the cause of IGT. The increase in type and frequency of mtDNA deletions in diabetes and IGT patients may be related to oxidative damage by oxygen free radicals. These may be produced in greater amounts as a result of hyperglycemia or may be more abundant because of an abnormality in the scavenging of free radicals by antioxidants.
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May 01 1997
Increased Prevalence of Mitochondrial DNA Deletions in Skeletal Muscle of Older Individuals With Impaired Glucose Tolerance: Possible Marker of Glycemic Stress
Ping Liang;
Ping Liang
Department of Medicine and the General Clinical Research Center, University of Vermont College of Medicine and Fletcher Allen Health Care
Burlington, Vermont
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Virginia Hughes;
Virginia Hughes
Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging at Tufts University
Boston, Massachusetts
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Naomi K Fukagawa
Naomi K Fukagawa
Department of Medicine and the General Clinical Research Center, University of Vermont College of Medicine and Fletcher Allen Health Care
Burlington, Vermont
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Address correspondence and reprint requests to Dr. Naomi K. Fukagawa, University of Vermont College of Medicine, Department of Medicine, Given C-207, Burlington, VT 05405–0068. [email protected].
1
IGT, impaired glucose tolerance; OGTT, oral glucose tolerance test; PCR, polymerase chain reaction; USDA, U.S. Department of Agriculture; UVM, University of Vermont.
2
The contents of this publication do not necessarily reflect the views or policies of the U.S. Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government.
Diabetes 1997;46(5):920–924
Article history
Received:
December 19 1996
Revision Received:
February 13 1997
Accepted:
February 13 1997
PubMed:
9133566
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Citation
Ping Liang, Virginia Hughes, Naomi K Fukagawa; Increased Prevalence of Mitochondrial DNA Deletions in Skeletal Muscle of Older Individuals With Impaired Glucose Tolerance: Possible Marker of Glycemic Stress. Diabetes 1 May 1997; 46 (5): 920–924. https://doi.org/10.2337/diab.46.5.920
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