Zucker fatty (fa/fa) rats exhibit overt obesity, hypercholesterolemia, hyperlipidemia, and hyperglycemia as recessive traits. The fa mutation has been determined to be a missense mutation in the extracellular domain of the leptin receptor. We report herein the construction of CHO cells that stably express the fa-type leptin receptor and the characterization of this receptor using mRNA expression levels of the immediate early genes, c-fos, c-jun, and jun-B, which are induced by leptin as a criterion of signal transduction. The fa-type receptor not only exhibits a slightly reduced leptinbinding affinity, but also performs reduced signal transduction.
Leptin Receptor of Zucker Fatty Rat Performs Reduced Signal Transduction
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Tatsuya Yamashita, Takashi Murakami, Mitsuru Iida, Masamichi Kuwajima, Kenji Shima; Leptin Receptor of Zucker Fatty Rat Performs Reduced Signal Transduction. Diabetes 1 June 1997; 46 (6): 1077–1080. https://doi.org/10.2337/diab.46.6.1077
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