The autosomal dominant neurological syndrome of Huntington's disease has been modeled in transgenic mice by the expression of a portion of the human huntingtin gene together with 140 CAG repeats (the R6/2 strain). The mice develop progressive chorea with onset at approximately 9 weeks of age and with death at approximately 13 weeks. Associated symptoms include weight loss and polyuria in the absence of eating or drinking deficits. We have found that these mice have insulin-responsive diabetes. Fasting glucose was 211 + 19 mg/dl in R6/2 mice compared with 93 + 5 mg/dl in C57/B6 controls (n = 12, both groups; P < 0.01). Administration of insulin intraperitoneally led to a reduction in blood glucose. At 12.5 weeks, animals were killed and pancreas weighed and analyzed for insulin and glucagon. Pancreatic mass in R6/2 mice was the same as controls, and islets appeared normal in morphology without lymphocytic infiltration. Immunohistochemical staining showed dramatic reductions in glucagon in the alpha-cells and in insulin in the beta-cells. Direct tissue assays showed glucagon and insulin content were reduced to only 10 and 15% of controls, respectively. Diabetes has been reported as being more common in Huntington's disease and other triplet repeat disorders. The R6/2 mouse should prove useful for elucidating the mechanism of diabetes in these genetic diseases.
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Abstract|
March 01 1999
Mice transgenic for an expanded CAG repeat in the Huntington's disease gene develop diabetes.
M S Hurlbert;
M S Hurlbert
Department of Medicine, University of Colorado Health Sciences Center, Denver, USA.
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W Zhou;
W Zhou
Department of Medicine, University of Colorado Health Sciences Center, Denver, USA.
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C Wasmeier;
C Wasmeier
Department of Medicine, University of Colorado Health Sciences Center, Denver, USA.
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F G Kaddis;
F G Kaddis
Department of Medicine, University of Colorado Health Sciences Center, Denver, USA.
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J C Hutton;
J C Hutton
Department of Medicine, University of Colorado Health Sciences Center, Denver, USA.
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C R Freed
C R Freed
Department of Medicine, University of Colorado Health Sciences Center, Denver, USA.
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Citation
M S Hurlbert, W Zhou, C Wasmeier, F G Kaddis, J C Hutton, C R Freed; Mice transgenic for an expanded CAG repeat in the Huntington's disease gene develop diabetes.. Diabetes 1 March 1999; 48 (3): 649–651. https://doi.org/10.2337/diabetes.48.3.649
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