Whalen BJ, Marounek J, Weiser P, Appel MC, Greiner DL, Mordes JP, Rossini AA: BB rat thymocytes cultured in the presence of islets lose their ability to transfer autoimmune diabetes.
On pages 974 and 975 of the above article, Figs. 1 and 2 were mistakenly reversed. The figure on page 974 corresponds with the Fig. 2 legend on page 975, and the figure on page 975 corresponds with the Fig. 1 legend on page 974. The correct figures and legends are shown on the following page.
Adoptive transfer of diabetes by fresh and cultured thymocytes. WAG-rnu/rnu rats were randomized, injected with either fresh or cultured BBDR thymocytes, and then treated with a combination of anti-ART2a mAb and poly I:C as described in research design and methods. A: Recipients in group A received the equivalent of one-fourth of a thymus, 2.0–2.5 × 108 cells. Recipients in group B received the equivalent of either one-fourth or one-half of a thymus (50–132 × 106 cells) cultured for 3–4 days. In a preliminary analysis, it was determined that there were no statistically significant differences between the outcomes obtained using one-fourth or one-half of a thymus, and the data for these two treatment conditions have been combined. Recipients in group C received the equivalent of either one-fourth or one-half of a thymus (10–52 × 106 cells) cultured for either 5–7 days. In a preliminary analysis, it was determined that there were no statistically significant differences between the outcomes obtained using one-fourth or one-half of a thymus, and the data for these two treatment conditions have been combined. Recipients in group D received cells from the equivalent of one-fourth of a thymus (5–7 × 106 cells) cultured for 9–10 days. Each curve represents the results of two independent trials. The cumulative frequency of diabetes was statistically similar in the recipients of fresh thymocytes (group A) and thymocytes after 3–4 days of culture (group B). The cumulative frequency of diabetes in the recipients of fresh thymocytes (group A) was significantly greater than that in recipients of cells from either 5- to 7-day (P < 0.005) or 9- to 10-day (P < 0.01) cultures. B: Recipients in groups E and F received the equivalent of either one-fourth (5 × 106 cells) or three-fourths of a thymus (15 × 106 cells) that had been cultured for 13–14 days. The curve for group E represents the pooled results of two independent trials; the curve for group F represents the results of a single trial. The cumulative frequency of diabetes in the two groups differs at the P < 0.001 level. Numbers of recipients are given in parentheses. Censored data (animals not diabetic at the time of death or termination of the experiment) are indicated by vertical bars.
Adoptive transfer of diabetes by fresh and cultured thymocytes. WAG-rnu/rnu rats were randomized, injected with either fresh or cultured BBDR thymocytes, and then treated with a combination of anti-ART2a mAb and poly I:C as described in research design and methods. A: Recipients in group A received the equivalent of one-fourth of a thymus, 2.0–2.5 × 108 cells. Recipients in group B received the equivalent of either one-fourth or one-half of a thymus (50–132 × 106 cells) cultured for 3–4 days. In a preliminary analysis, it was determined that there were no statistically significant differences between the outcomes obtained using one-fourth or one-half of a thymus, and the data for these two treatment conditions have been combined. Recipients in group C received the equivalent of either one-fourth or one-half of a thymus (10–52 × 106 cells) cultured for either 5–7 days. In a preliminary analysis, it was determined that there were no statistically significant differences between the outcomes obtained using one-fourth or one-half of a thymus, and the data for these two treatment conditions have been combined. Recipients in group D received cells from the equivalent of one-fourth of a thymus (5–7 × 106 cells) cultured for 9–10 days. Each curve represents the results of two independent trials. The cumulative frequency of diabetes was statistically similar in the recipients of fresh thymocytes (group A) and thymocytes after 3–4 days of culture (group B). The cumulative frequency of diabetes in the recipients of fresh thymocytes (group A) was significantly greater than that in recipients of cells from either 5- to 7-day (P < 0.005) or 9- to 10-day (P < 0.01) cultures. B: Recipients in groups E and F received the equivalent of either one-fourth (5 × 106 cells) or three-fourths of a thymus (15 × 106 cells) that had been cultured for 13–14 days. The curve for group E represents the pooled results of two independent trials; the curve for group F represents the results of a single trial. The cumulative frequency of diabetes in the two groups differs at the P < 0.001 level. Numbers of recipients are given in parentheses. Censored data (animals not diabetic at the time of death or termination of the experiment) are indicated by vertical bars.
Adoptive transfer of diabetes by thymocytes cultured in the presence or absence of antigen. WAG-rnu/rnu rats were randomized, injected with either fresh or cultured BBDR thymocytes, and then treated with a combination of anti-ART2a mAb and poly I:C as described in research design and methods. Recipients were injected with thymocytes cultured in the presence or absence of BBDR rat pancreatic islets or BBDR rat thyrocytes. Islets and thyroids were prepared as described in research design and methods. A: Cells were transfused into recipients after 4 or 5 days of culture. Each recipient received the equivalent of one-half of a cultured thymus. The number of cells transfused was 72–133 × 106 for day 4 cultures and 52–67 × 106 for day 5 cultures. In a preliminary analysis, it was determined that there were no statistically significant differences between the outcomes obtained using day 4 and day 5 cultures for any of the three experimental groups, and the data for day 4 and 5 cultures are combined. Each curve represents the results of three independent trials. The cumulative frequency of diabetes in recipients of thymocytes cultured in the presence of islets is significantly less than that in recipients of control thymocytes (P < 0.005) or thyrocytes (P < 0.001). Frequency of diabetes in recipients of thymocytes cultured in the presence of thyrocytes is statistically similar to that in recipients of control thymocytes (P = 0.8). B: Cells were transfused into recipients after 7 days of culture. Each recipient received the equivalent of one-half of a cultured thymus. The number of cells transfused was 26–31 × 106. Each curve represents the results of two independent trials. The cumulative frequency of diabetes in recipients of cells cultured in the presence of either fresh, frozen, or STZ-treated islets is statistically similar. The cumulative frequency of diabetes in recipients of cells cultured in the presence of islets is significantly less than that in recipients of either control or thyrocyte-treated ATOC (P < 0.001 for both comparisons). Frequency of diabetes in recipients of thymocytes cultured in the presence of thyrocytes is statistically similar to that in recipients of control thymocytes (P = 0.15). In addition, the frequency of diabetes in recipients of thymocytes cultured in the presence of thyrocytes in both A and B is statistically similar (P = 0.98). Censored data (animals not diabetic at the time of death or termination of the experiment) are indicated by vertical bars.
Adoptive transfer of diabetes by thymocytes cultured in the presence or absence of antigen. WAG-rnu/rnu rats were randomized, injected with either fresh or cultured BBDR thymocytes, and then treated with a combination of anti-ART2a mAb and poly I:C as described in research design and methods. Recipients were injected with thymocytes cultured in the presence or absence of BBDR rat pancreatic islets or BBDR rat thyrocytes. Islets and thyroids were prepared as described in research design and methods. A: Cells were transfused into recipients after 4 or 5 days of culture. Each recipient received the equivalent of one-half of a cultured thymus. The number of cells transfused was 72–133 × 106 for day 4 cultures and 52–67 × 106 for day 5 cultures. In a preliminary analysis, it was determined that there were no statistically significant differences between the outcomes obtained using day 4 and day 5 cultures for any of the three experimental groups, and the data for day 4 and 5 cultures are combined. Each curve represents the results of three independent trials. The cumulative frequency of diabetes in recipients of thymocytes cultured in the presence of islets is significantly less than that in recipients of control thymocytes (P < 0.005) or thyrocytes (P < 0.001). Frequency of diabetes in recipients of thymocytes cultured in the presence of thyrocytes is statistically similar to that in recipients of control thymocytes (P = 0.8). B: Cells were transfused into recipients after 7 days of culture. Each recipient received the equivalent of one-half of a cultured thymus. The number of cells transfused was 26–31 × 106. Each curve represents the results of two independent trials. The cumulative frequency of diabetes in recipients of cells cultured in the presence of either fresh, frozen, or STZ-treated islets is statistically similar. The cumulative frequency of diabetes in recipients of cells cultured in the presence of islets is significantly less than that in recipients of either control or thyrocyte-treated ATOC (P < 0.001 for both comparisons). Frequency of diabetes in recipients of thymocytes cultured in the presence of thyrocytes is statistically similar to that in recipients of control thymocytes (P = 0.15). In addition, the frequency of diabetes in recipients of thymocytes cultured in the presence of thyrocytes in both A and B is statistically similar (P = 0.98). Censored data (animals not diabetic at the time of death or termination of the experiment) are indicated by vertical bars.
