Type 2 diabetes was once referred to as a “geneticist’s nightmare” (1) due to difficulties stemming from the nature of the disease and the strategies available for genetic analysis. Two key genetic approaches previously utilized—linkage analysis and candidate gene association studies—each have limitations that have been difficult to overcome in the study of type 2 diabetes. Linkage studies have been hampered by difficulties in obtaining well-defined pedigrees because of the late onset of the disease, imprecise diagnostic criteria, and genetic heterogeneity. Association studies are limited in scope to the consideration of known genes that have an established biological link to diabetes.

One approach to overcome some of these difficulties is to couple genome-wide linkage analysis in genetically isolated populations with high-resolution typing of genetic variations across the linkage region. This was shown to be a viable approach for the identification of novel diabetes genes with the discovery of variants in TCF7L2, a gene encoding a ubiquitous transcription factor with no previous functional connection to diabetes (2). Importantly, subsequent studies have revealed a previously unknown role for TCF7L2 in pancreatic β-cells, with the risk allele leading to altered TCF7L2 expression levels and reduced insulin secretion (3), although the mechanism by which the intronic polymorphism functions is not clear.

In this issue of Diabetes, a similar combined genetic linkage–association strategy has identified an association between LPIN2 and insulin sensitivity and BMI (4). Previous genome-wide linkage studies identified a region of chromosome 18p11 linked to type 2 diabetes in populations from Finland, Sweden, and the Netherlands (5,6). In the current report, markers across this region were typed and the strongest association with diabetes localized to a single nucleotide polymorphism (SNP) residing in the 3′ untranslated region of LPIN2. Interestingly, this variant was shown to interact with BMI in determination of type 2 diabetes. Thus, the common allele was associated with increased diabetes risk in individuals with high BMI but with neutral or protective effects in lean individuals (4).

LPIN2 encodes one of three members of the lipin protein family, which have dual functions as phosphatidate phosphatase-1 enzymes required for triglyceride and phospholipid biosynthesis and as transcriptional coactivators for peroxisome proliferator–activated receptor nuclear receptors (79). The founding member of the family, lipin-1, was identified in a mutant mouse model of lipodystrophy characterized by adipose tissue deficiency and insulin resistance (10). In humans, LPIN1 polymorphisms are associated with insulin levels and BMI (11). Lipin-1 is expressed at high levels in adipose tissue and skeletal muscle, and enhanced expression in either tissue leads to obesity but with opposite effects on insulin sensitivity (12). In mice and humans, lipin-1 levels in adipose tissue are correlated with insulin sensitivity, whereas lipin-1 levels in mouse muscle correlate with insulin resistance (1113). Thus, it appears that lipin-1 levels in multiple tissues influence glucose homeostasis. Lipin-2 appears to have molecular functions similar to those of lipin-1 but exhibits a distinct tissue expression pattern, with higher levels in liver and lower levels in brain, small intestine, kidney, and lung (7). The fact that lipin-1–deficient mice have normal hepatic phosphatidate phosphatase-1 activity suggests that lipin-2 and/or lipin-3 may be responsible for this activity in liver.

What are the implications regarding lipin-2 in the pathogenesis of type 2 diabetes? It is tempting to speculate that the genetic polymorphism identified may influence hepatic triglyceride levels and potentially the degree of hepatic steatosis and insulin resistance in conditions such as obesity. Lipin-2 may also have an important role in regulation of the inflammatory response, as revealed by LPIN2 mutations in Majeed syndrome. Majeed syndrome is an autoinflammatory disorder with a constellation of symptoms including recurrent fever, sterile osteomyelitis, cutaneous inflammation, and anemia (14,15). The mechanism by which lipin-2 mutations cause these symptoms is not known, and no information regarding insulin resistance or diabetes in Majeed patients has been reported.

The physiological roles of lipin-2 in lipid metabolism and inflammation make LPIN2 an intriguing and plausible diabetes gene. However, further studies will be required to establish whether the SNP identified is functionally associated with diabetes or is simply in linkage disequilibrium with the causal variant in LPIN2 or a neighboring gene. The initial studies failed to detect any effect of the LPIN2 polymorphism on mRNA levels in white blood cells or in micro-RNA binding to a predicted target sequence that contains the SNP (4). These issues can be resolved in future genetic, molecular, and physiological studies. For example, additional analysis of the potential effects of the LPIN2 SNP on mRNA levels and function should be performed in relevant cell types, such as cultured hepatocytes or liver from mouse models. Further analysis of the region via deep sequencing can be used to identify all common and potential functional variants, and physiological studies of lipin-2 function in diabetes can be carried out in genetically manipulated mouse models.

The implication of two members of the lipin gene family as determinants of insulin sensitivity serves to underscore the close relationship between lipid homeostasis and diabetes. Now, what about lipin-3?

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