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In the print version of the article listed above, some symbols were inadvertently omitted from the legend for Fig. 2. The correct legend appears below.
FIG. 2. Elevated cAMP levels induce oligomycin-insensitive OCR. Representative measurements of the percent change in OCR in human adipocytes after injections of DMEM (open symbols) or the inhibitors (closed symbols): ATP synthase inhibitor oligomycin (Oligo, 1 μg/ml) or the complex 1 inhibitor, rotenone (Rot, 3 μmol/l) as indicated by the dashed vertical lines. Oligo was injected 30–40 min after DMEM (CONT, circles) or (A) isoproterenol (ISO, 1 μmol/l, squares), (B) forskolin (FSK, 10 μmol/l, triangles), (C) dibutyryl-cAMP (DB, 1 mmol/l, diamonds). OCR before Oligo injection was set as 100%. The results are from adipocytes pooled from five subjects, and each data point is a mean of 4–5 wells. D: Histogram summarizing the percent decrease in OCR in response to Oligo or Rot injections. Data are relative to OCR levels before Oligo injection (and after ISO, FSK, or DB injections). The data are an average of 3–5 experiments using a total of 15 subjects (BMI range 21.7–35.5 kg/m2). *P < 0.001, compared with untreated CONT; ^P < 0.01, compared with untreated ISO; #P < 0.001, compared with CONT + Oligo. E: The relative changes in OCR between Oligo and Rot are expressed as % uncoupling (calculated from the results presented in D, dotted arrows).
FIG. 2. Elevated cAMP levels induce oligomycin-insensitive OCR. Representative measurements of the percent change in OCR in human adipocytes after injections of DMEM (open symbols) or the inhibitors (closed symbols): ATP synthase inhibitor oligomycin (Oligo, 1 μg/ml) or the complex 1 inhibitor, rotenone (Rot, 3 μmol/l) as indicated by the dashed vertical lines. Oligo was injected 30–40 min after DMEM (CONT, circles) or (A) isoproterenol (ISO, 1 μmol/l, squares), (B) forskolin (FSK, 10 μmol/l, triangles), (C) dibutyryl-cAMP (DB, 1 mmol/l, diamonds). OCR before Oligo injection was set as 100%. The results are from adipocytes pooled from five subjects, and each data point is a mean of 4–5 wells. D: Histogram summarizing the percent decrease in OCR in response to Oligo or Rot injections. Data are relative to OCR levels before Oligo injection (and after ISO, FSK, or DB injections). The data are an average of 3–5 experiments using a total of 15 subjects (BMI range 21.7–35.5 kg/m2). *P < 0.001, compared with untreated CONT; ^P < 0.01, compared with untreated ISO; #P < 0.001, compared with CONT + Oligo. E: The relative changes in OCR between Oligo and Rot are expressed as % uncoupling (calculated from the results presented in D, dotted arrows).
The online version reflects these changes.
