Gutierrez DA, Kennedy A, Orr JS, Anderson EK, Webb CD, Gerrald WK, Hasty AH. Aberrant accumulation of undifferentiated myeloid cells in the adipose tissue of CCR2-deficient mice delays improvements in insulin sensitivity. Diabetes 2011;60:
During a recent examination of the data from the publication listed above, we realized that we erroneously mislabeled the blood neutrophil population as the Ly6Clo monocyte population in Supplementary Figs. 2–4. The accidental mislabeling of these populations does not in any way affect the interpretation of the results or the outcome of the study. In this study, we were particularly interested in the levels of Ly6Chi monocytes, and this population was correctly gated and labeled in the original figures. We reported that there was a small increase in the blood Ly6Clo monocytes in the BM-CCR2−/− mice. However, we gated on the neutrophil population and not the Ly6Clo population. The new quantification of the correct population is shown below in Supplementary Fig. 2D. It is important to note that we did not take into consideration the levels of Ly6Clo monocytes when interpreting our data. The online version reflects these changes.
BM-CCR2−/− mice have decreased levels of circulating inflammatory monocytes. White blood cells were isolated from BM-CCR2+/+ and BM-CCR2−/− mice fed an HFD for 12 weeks. Circulating monocyte levels were assessed by flow cytometry by first gating on all live CD11b+ cells. Representative pseudo-color flow cytometry plot of Ly6C expression in (A) BM-CCR2+/+ controls and (B) BM-CCR2−/− mice. Quantification of the percentage of circulating (C) Ly6Chi monocytes and (D) Ly6Clo monocytes in BM-CCR2+/+ and BM-CCR2−/− mice (mean ± SEM; n = 4 mice per group).
BM-CCR2−/− mice have decreased levels of circulating inflammatory monocytes. White blood cells were isolated from BM-CCR2+/+ and BM-CCR2−/− mice fed an HFD for 12 weeks. Circulating monocyte levels were assessed by flow cytometry by first gating on all live CD11b+ cells. Representative pseudo-color flow cytometry plot of Ly6C expression in (A) BM-CCR2+/+ controls and (B) BM-CCR2−/− mice. Quantification of the percentage of circulating (C) Ly6Chi monocytes and (D) Ly6Clo monocytes in BM-CCR2+/+ and BM-CCR2−/− mice (mean ± SEM; n = 4 mice per group).
CCR2−/− mice have decreased levels of blood Ly6Chi monocytes and increased expression of CD11c in Ly6Clo monocytes. White blood cells were isolated from CCR2+/+ and CCR2−/− mice fed an HFD for 12 weeks. Circulating monocyte levels were assessed by flow cytometry by first gating on all live CD11b+ cells. A: Gates on Ly6Chi and Ly6Clo populations in CCR2+/+ mice. B: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2+/+ mice. C: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2+/+ mice. D: Gates on Ly6Chi and Ly6Clo populations in CCR2−/− mice. E: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2−/− mice. F: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2−/− mice. “N” in panels A and D indicates neutrophil populations.
CCR2−/− mice have decreased levels of blood Ly6Chi monocytes and increased expression of CD11c in Ly6Clo monocytes. White blood cells were isolated from CCR2+/+ and CCR2−/− mice fed an HFD for 12 weeks. Circulating monocyte levels were assessed by flow cytometry by first gating on all live CD11b+ cells. A: Gates on Ly6Chi and Ly6Clo populations in CCR2+/+ mice. B: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2+/+ mice. C: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2+/+ mice. D: Gates on Ly6Chi and Ly6Clo populations in CCR2−/− mice. E: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2−/− mice. F: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CCR2−/− mice. “N” in panels A and D indicates neutrophil populations.
HFD feeding increases the numbers and the CD11c expression of Ly6Chi monocytes in CCR2+/+ mice. White blood cells were isolated from CCR2+/+ mice fed an HFD or CD for 12 weeks. Circulating monocyte levels were assessed by flow cytometry by first gating on all live CD11b+ cells. A: Gates on Ly6Chi and Ly6Clo populations in HFD-fed CCR2+/+ mice. B: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in HFD-fed CCR2+/+ mice. C: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in HFD-fed CCR2+/+ mice. D: Gates on Ly6Chi and Ly6Clo populations in CD-fed CCR2+/+ mice. E: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CD-fed CCR2+/+ mice. F: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CD-fed CCR2+/+ mice. “N” in panels A and D indicates neutrophil populations.
HFD feeding increases the numbers and the CD11c expression of Ly6Chi monocytes in CCR2+/+ mice. White blood cells were isolated from CCR2+/+ mice fed an HFD or CD for 12 weeks. Circulating monocyte levels were assessed by flow cytometry by first gating on all live CD11b+ cells. A: Gates on Ly6Chi and Ly6Clo populations in HFD-fed CCR2+/+ mice. B: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in HFD-fed CCR2+/+ mice. C: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in HFD-fed CCR2+/+ mice. D: Gates on Ly6Chi and Ly6Clo populations in CD-fed CCR2+/+ mice. E: Histogram of CD11c expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CD-fed CCR2+/+ mice. F: Histogram of F4/80 expression in Ly6Chi (red line) and Ly6Clo (blue line) monocytes in CD-fed CCR2+/+ mice. “N” in panels A and D indicates neutrophil populations.
