Duodenal-jejunal bypass surgery (DJB) is a experimental surgical procedure to study the mechanism of gastric surgery in T2DM treatment.

Methods: T2DM rats induced by high glucose high fat food and low dose streptozotocin (STZ) injection were randomly divided into 2 groups: T2DM group and DJB surgery group (T2DM-DJB).Wistar rats were used as normal control. The fasting blood glucose and HbA1C were measured. Glucose utilizationin the brain was detected by small animal positron emission tomography (PET) using [18F]-fluorodeoxyglucose ([18F]-FDG).GLUT1, GLUT3 expression in the brain were measured by Q-RT-PCR.

Results:DJB surgery significantly decreased the fasting blood glucose and Hb1Ac of the T2DM rats. [18F]-FDG uptake (standardized uptake values (SUV)mean)in the brain was elevated siginificantly 4 weeks after DJB (Wistar vs. T2DM vs. T2DM-DJB: SUVmean value 1.30±0.27 vs. 0.59±0.10 vs. 1.55±0.31, P<0.001). Q-RT-PCR showed that GLUT3 expression in the brain significantly increased 4 weeks after DJB while T2DM rats decreased. This study indicates that the improvement of glucose utilization in the brain plays an pivotal role in DJB treatment of T2DM.


R. Pan: None. N. Li: None. T. Zhao: None. H. Wang: None. B. Jiang: None. Q. Yan: None. Z. Gao: None. M. Qu: None.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at http://www.diabetesjournals.org/content/license.