1752-P: GDF11 Involved in Expressed MicroRNAs during GLP-1RA Inhibited Adipogenesis

Differentiation of bone marrow mesenchymal stem cells (BMSCs)is controlled by a vast array of mechanisms, with miRNAs-mRNAs regulation network identified widely recently. In this study, we aimed to specify the differentially expressed (DE) miRNAs after glucagon-like peptide-1 receptor agonist (GLP-1RA) administration during adipogenesis in BMSCs. MiRNAs extracted from three different groups, [the control group (C), the adipogenesis group (A), and the GLP-1RA administration group (GA)], were sent for high-throughput sequencing. We identified 5 DE miRNAs (miRNA-150-5p, miRNA-129-5p, miRNA-201-3p, miRNA-201-5p, and miRNA-214-5p) among groups. The expression of the 5 DE miRNAs were verified by the real-time RT-PCR analysis and consistent with the small-RNA sequencing data. Target genes were then predicted, and highly enriched GOs and KEGG pathway analyses indicated that they were involved in multiple biological processes concerning adipogenesis. Two of the target genes, growth differentiation factor 11 (GDF11) and cholecystokinin B receptor (CCKBR), were verified for functional study. Actually, the mRNA levels of GDF11, rather than CCKBR, was down-regulated by miRNA-150-5pand involved in GLP-1RA administration. Taken together, these results suggested for the first time that GDF11 was involved in differentially expressed microRNAs during GLP-1RA inhibited adipogenesis in primary BMSCs.