2054-P: Endothelial Dysfunction, Inflammation, and Oxidative Stress Urinary Biomarkers in Obese Children from Alabama

Obesity is a state of chronic low-level inflammation closely associated with the oxidative stress. Childhood obesity has been related to endothelial dysfunction, inflammation and oxidative stress markers individually. The aim of this study was to determine the association of biomarkers of the inflammation, oxidative stress and endothelial dysfunction in urine samples of healthy and obese children. Thirty healthy and thirty overweight/obese elementary school children aged between 6 to 10 years from Alabama participated in this study. Anthropometric measurements were measured using WHO recommendations. Urine samples were analyzed for biomarkers of low-grade inflammation such as interleukin-6 (IL-6), C-reactive protein (CRP), α-1-acid glycoprotein (AGP); oxidative stress such as 8-isoprostane, 8-hydroxy-2'-deoxyguanosine (8-OHdG); and endothelin-1 (ET-1). Linear regression analysis was performed to analyze the association between the parameters. The overweight/obese children have significantly increased levels of IL-6, CRP, ET1 and 8-isoprostane compared to normal weight. CRP, IL-6, AGP and 8-isoprostane have significant positive association with at least one of the obesity measures except the ET-1 and 8-OHdG. ET-1 is significantly positively correlated with IL-6 and 8-isoprostane. The increased urinary IL-6 and 8-isoprostane can be used as a non-invasive benchmark to monitor childhood obesity.