2493-PUB: Distinct 5-Methylcytosine Profiles in Ribo-Minus RNA from Mouse Brown Adipose Tissue

Methylation of DNA and RNA has become a research hotspot because of its diverse regulation of biological function and processes. In particular, posttranscriptional modifications in RNA, including methylation of the N6 positions in adenine (m⁶A), N1 positions in adenine (m¹A) and methylation of carbon 5 in cytosine (m⁵C). However, information about the transcriptome-wide distribution and possible functions of m⁵C in different cell types, tissues, and organisms is still extremely limited. Brown adipose tissue is a functional organ for thermogenesis and an important potential target for the treatment of obesity and diabetes. The insights into possible functions of m⁵C modification and implications in BAT are significant and urgent to be revealed. In our study, we performed an unbiased global analysis of m⁵C in total and Ribo-minus RNA of brown adipose tissue from mouse after thermoneutrality (29?-30?) and/or cold stimulation (4?) for 1 day. We identified total 8153 sites in mRNA, 570 sites in CircRNA, and 991 sites in LncRNA, with respect to the degree of methylation, functional classification of methylated transcripts, and position bias within the transcript. Interestingly, there are intriguing differences in BAT after thermoneutrality and/or cold stimulation, which may provide us with new ideas to activate or enhance functionality of BAT. Our findings show the first comprehensive picture of m⁵C in the BAT from the mouse. These data establish and provide an important resource for future studies of function and biological significance of m⁵C in Ribo-minus RNA in brown adipose tissue.