Diabetic foot is a common reason of foot ulcer and amputation. Studies found GLP-1 receptor agonist (GLP-1RA) enhances the angiogenesis of endothelial progenitor cells (EPCs) on ischemic lower limbs in diabetic mice. But the cause is unclear. The blood perfusion and capillary density are observed in diabetic mice after the treat of GLP-1RA by establishing a model of ischemic hind limb. Primary EPCs being isolated and cultured we observed the number of peripheral EPCs the NO stimulated by GLP-1RA. The results that GLP-1RA enhance blood perfusion and capillary density of ischemic hind limbs.

Conclusion, High glucose decrease the expression of GLP-1R in EPCs and angiogenesis and migration of EPCs while the remedy of GLP-1RA Reverse this phenomenon.(A)The LDPI index were significantly higher in the liralutide group than that in the Vehicle group (N=8-10 per group).**:p<0.01, ***:p<0.001. (B) Quantitative analysis of (A). (C) The angiogenesis ability of EPCs was evaluated using a Matrigel-like structure formation assay. (D)Quantitative analysis of (C). the high glucose significantly impaired the angiogenic ability of EPCs, while GLP-1RA. Reverse this phenomenon, and this improvement showed a dose effect.**:p<0.01. (N=3-4 per group)(E). The results that high glucose significantly impaired the migration ability of EPCs, while GLP-1RA Reverse this phenomenon. **: p<0.01.(N=4 per group) (F) Quantitative analysis of (E).


Z. Chao: None.

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