Objective: Since type 2 diabetes (DM) is a life-style related disease, life-style should be considered, when association between genetic factors and DM are examined. However, most studies did not examine genetic associations with such consideration, as the case of glucagon-like peptide-1 receptor (GLP-1R) gene. We examined the association in consideration with the interaction between the gene polymorphism and various nutrient factors.

Research Design and Methods: Among participants of the population-based Iwaki study of Japanese held in 2014-2017, those with information of the genotype of GLP-1R polymorphism (db SNP ID: rs3765467: A/G)(p.Alg131Gln) as well as nutrients consumed (n=1817) were included. The following individuals were also excluded: 64 on medication for DM, and 52 with FBG levels below 63 mg/dl or over 140 mg/dl (to evaluate HOMA indices precisely). After these exclusions, 1,560 individuals (587 men, 973 women) aged 53.3± 16.1 years were included in the study.

Results: Though not significant, insulin secretion indices assed by homeostasis model assessment (HOMA- ß) in subjects with the GG genotype tended to be lower than in those with the AA+AG genotypes in most groups stratified into tertiles based on their daily nutrients consumptions (high, middle, and low groups). The stratification also showed that the genotype GG was a significant risk for a decreased insulin secretion (HOMA-ß ≤ 30) even after adjustment for multiple factors (age, BMI, drinking alcohol) only in the highest tertiles of energy, protein and carbohydrate consumption in men (odds ratios (95% CI) in high energy, protein and carbohydrate consumption groups: 3.95 (1.03-15.1), 15.83 (1.58-158.9), and 4.23 (1.10-11.2), respectively).

Conclusions: The GLP-1R gene was associated with a decreased insulin secretion dependently of nutrients consumption, which indicates interaction between GLP-1R and nutrient factors on pathophysiology leading to DM.


Y. Nishiya: None. M. Daimon: None. H. Murakami: None. M. Murabayashi: None. S. Mizushiri: None. T. Fujita: None. Y. Matsuhashi: None.

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