1172-P: Estimation of Glucose Turnover Using Naturally Enriched Complex Carbohydrate Meal in Type 2 Diabetes: Comparison with Triple-Tracer Meal

We have recently established a method utilizing the natural abundance of [¹³C] polysaccharide enabling measurement of enrichment of [¹³C] glucose in healthy humans. We now compare complex carb (CC) meal with a single tracer infusion of [6,6-²H₂] glucose at -120 min to the established simple carb (SC) triple tracer protocol with infusion of [6,6-²H₂] glucose at -120 min and [2-¹³C] glucose at 0 min with meal ingested at time 0. Six type 2 diabetes subjects (4M, age 65±6 y; BMI 33±4 kg/m²; HbA1c 50±10 mM/mol) were studied in random order with SC labeled with [1-¹³C] glucose and CC meal with sorghum grain. All meals had ~75 g carbohydrates with matched macronutrient content. Steele’s non-steady state equations were used to calculate glucose fluxes: rates of meal glucose appearance (Ra_meal), glucose disappearance (Rd) and endogenous glucose production (EGP) (Results in Fig1). Glucose, insulin and C-peptide concentrations achieved as well as iAUC of Ra_meal and Rd were significantly lower with CC than SC meal (p<0.05). However, EGP was similar between groups, (p=0.07). The results provide new insights into post prandial glucose physiology with CC meals that are healthier option for patients with diabetes. We can now conduct successful single stable tracer infusion based measurement of postprandial fluxes with real-world CC containing mixed meals in people with type 2 diabetes.

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