Visual Abstract

Background: SSTR2a rescues glucagon counterregulation in type 1 diabetic rats. Here, we investigated its potential for resisting hypoglycemic onset in insulin-treated rats with T2D.

Methods: T2D was induced in 8-9-week-old, male, Sprague-Dawley rats (n=20) via 3-weeks of high-fat feeding and low-dose streptozotocin (35 mg/kg). After 1 week of basal insulin maintenance, N=10 healthy and N=20 T2D rats were treated with SSTR2a (3 mg/kg ZT-01, SC) or vehicle in a crossover design (1 wk apart), 60 min prior to hypoglycemic induction via insulin aspart bolus (6 U/kg).

Results: SSTR2a increased plasma glucagon levels for up to 120 min after insulin challenge, with a 2.2-fold increase in glucagon AUC (32167 ± 6658 vs. 14428 ± 3673 pg*min/mL; p<0.01). Blood glucose was significantly elevated in treated, T2D rats for ∼30 min, and hypoglycemia onset was delayed by 17 ± 4 min (p<0.001). Neither effect was observed in SSTR2a-treated, healthy rats.

Conclusions: SSTR2a increased glucagon secretion following insulin overdose and delayed the onset of hypoglycemia in T2D rats. SSTR2a may be a promising therapeutic tool for alleviating hypoglycemic risk in intensively-treated T2D.


E. G. Hoffman: None. N. Dsouza: None. J. Aiken: None. R. Liggins: Employee; Self; Zucara Therapeutics Inc. M. Riddell: Advisory Panel; Self; Zealand Pharma A/S, Consultant; Self; Lilly Diabetes, Research Support; Self; Dexcom, Inc., Insulet Corporation, Speaker’s Bureau; Self; Novo Nordisk Inc., Sanofi, Stock/Shareholder; Self; Zucara Therapeutics Inc.



Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at