Background: Inflammatory processes are dynamic over the Diabetic Foot Ulceration (DFU) course. We aimed to evaluate longitudinal trajectories of circulating biomarkers in subjects with DFU.

Methods: The study cohort comprised 39 subjects with DFU from the Joslin-Beth Israel Deaconess Foot Center. During the 12 weeks of follow-up (6 bi-weekly visits), 15 subjects (38%) had their DFU healed (healers). Twenty-eight proteins were measured at each visit on the Luminex platform.

Results: Circulating IL-10 levels at baseline were significantly higher in DFU healers in comparison with non-healers (OR: 2.4, p=0.047). Moreover, IL-10 levels remained stable over time within each group (IL10 Δ in DFU healers: p=0.2 and in non-healers: p=0.8). Levels of 6 other proteins revealed dynamic trajectories over time in one or both groups: MMP-2, EGF, IFNγ, TNF, IL-8, and MIP1b. MMP-2 levels in healers increased from baseline to V6 (β=0.07, p=0.0003), but remained stable in non-healers.

Conclusion: We identified an anti-inflammatory cytokine, IL-10, as a prognostic biomarker of the diabetic wound healing course. Six inflammatory proteins (MMP-2, EGF, IFNγ, TNF, IL-8, and MIP1b) revealed dynamic temporal changes in levels within or across groups.

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Disclosure

S.Pickett: None. S.Moon: None. G.Theocharidis: None. Z.Md dom: None. M.Niewczas: None. A.Veves: None.

Funding

National Institutes of Health (R61DK131915, R01DK091949); Joslin Diabetes Center; National Rongxiang Xu Foundation

© 2023 by the American Diabetes Association
2023
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