60-PUB: Simultaneous Analysis of Serum Monosaccharides Levels among Adults of Self-Reported Diabetes Status using Non-Derivatized LC-MS/MS Method

Introduction and Objective: Glucose is the major biomarker for diagnosis and management of diabetes. Fructose is sometimes used to replace glucose and reduce glucose intake. Increased consumption of fructose was shown to contribute to non-alcoholic fatty liver disease (NAFLD). To better understand the associations between monosaccharides levels in blood and metabolic diseases in human, a high-throughput LC-MS/MS method was developed for simultaneous measurement of monosaccharides.

Methods: In this study, we measured serum monosaccharides concentrations in 100 non-fasting adult donors with self-reported diabetes status using a LC-MS/MS method for glucose, fructose, mannose, and galactose. We evaluated the correlations among monosaccharide concentrations, self-reported age, gender, BMI, and diabetes status in donors.

Results: All monosaccharides, except for galactose, were detected in all blood samples. The mean glucose concentration from donors with self-reported diabetes was significantly higher than the donors without diabetes (164.53 mg/dL vs 90.23 mg/dL, p <0.001). The same distribution was observed for mannose concentration between the two groups (2.25 mg/dL with diabetes vs 1.30 mg/dL without diabetes, p <0.001). No significant difference for fructose concentration was observed between both groups. However, the mean ratios of fructose to glucose concentrations were significantly lower in donors with self-reported diabetes than those without (0.004 vs 0.014, p <0.001).

Conclusion: The use of our recently developed high-throughput LC-MS/MS can simultaneously measure concentrations of multiple monosaccharides in human serum. The method can be used to investigate for the roles of these monosaccharides in metabolic diseases in large epidemiological studies.