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atgl-adipose-triglyceride-lipase

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Effect of EPA on energy metabolism–related genes in the HF/HS groups. <ital
Published: 03 August 2010
FIG. 3. Effect of EPA on energy metabolism–related genes in the HF/HS groups. A and B: Liver. C and D: Skeletal muscle. E: Epididymal WAT. F: Hepatic SREBP-1 protein in the HF/HS group. n = 7–10. †P < 0.05; ††P < 0.01 vs. control group. *P < 0.05; **P < 0.01 vs. HF/HS group. ACO, acyl-CoA oxidase; ATGL, adipose triglyceride lipase; CPT-1a, carnitine palmitoyltransferase-1a; CS, citrate synthase; GK, glucokinase; HAD, hydroxyacyl-CoA dehydrogenase; HK-2, hexokinase-2; HSL, hormone-sensitive lipase; MCAD, acetyl-CoA dehydrogenase, medium chain; PFKL, phosphofructokinase, liver; PFKM, phosphofructokinase, muscle, B-type. FIG. 3. Effect of EPA on energy metabolism–related genes in the HF/HS groups. A and B: Liver. C and D: Skeletal muscle. E: Epididymal WAT. F: Hepatic SREBP-1 protein in the HF/HS group. n = 7–10. †P < 0.05; ††P < 0.01 vs. control group. *P < 0.05; **P < 0.01 vs. HF/HS group. ACO, acyl-CoA oxidase; ATGL, adipose triglyceride lipase; CPT-1a, carnitine palmitoyltransferase-1a; CS, citrate synthase; GK, glucokinase; HAD, hydroxyacyl-CoA dehydrogenase; HK-2, hexokinase-2; HSL, hormone-sensitive lipase; MCAD, acetyl-CoA dehydrogenase, medium chain; PFKL, phosphofructokinase, liver; PFKM, phosphofructokinase, muscle, B-type. More
Journal Articles
Journal: Diabetes
Diabetes 2006;55(5):1270–1275
Published: 01 May 2006
...Veit Schoenborn; Iris M. Heid; Caren Vollmert; Arno Lingenhel; Ted D. Adams; Paul N. Hopkins; Thomas Illig; Robert Zimmermann; Rudolf Zechner; Steven C. Hunt; Florian Kronenberg Adipose triglyceride lipase (ATGL) was recently described to predominantly perform the initial step...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2006;55(1):148–157
Published: 01 January 2006
...Erin E. Kershaw; Jonathan K. Hamm; Linda A.W. Verhagen; Odile Peroni; Masa Katic; Jeffrey S. Flier Adipose triglyceride lipase (ATGL) is a recently described adipose-enriched protein with triglyceride-specific lipase activity. ATGL shares the greatest sequence homology with adiponutrin...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(11):3190–3197
Published: 01 November 2005
... by hormone-sensitive lipase (HSL) and the recently characterized adipose triglyceride lipase (ATGL), yet their relative importance in lipolysis is unknown. We show that a novel potent inhibitor of HSL does not inhibit other lipases. The compound counteracted catecholamine-stimulated lipolysis in mouse...
Includes: Supplementary data
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Selectivity of BAY for inhibition of HSL. <em>A</em>: Concentration...
Published: 01 November 2005
FIG. 1. Selectivity of BAY for inhibition of HSL. A: Concentration-response inhibition by BAY of purified human HSL (hHSL) or rat HSL (rHSL), pancreatic lipase (PL), lipoprotein lipase (LPL), and monoglyceride lipase (MGL) enzymatic activities (n = 3). B: BAY (10−6 mol/l) inhibition of triglyceride hydrolase activity in Cos7 cells expressing human HSL or human ATGL (n = 3). C: Concentration-response inhibition by BAY of mouse adipose tissue extract triglyceride hydrolase activity (n = 3). D: BAY (10−6 mol/l) inhibition of triglyceride hydrolase activity in wild-type and HSL-null mouse adipose tissue extracts (n = 4). FIG. 1. Selectivity of BAY for inhibition of HSL. A: Concentration-response inhibition by BAY of purified human HSL (hHSL) or rat HSL (rHSL), pancreatic lipase (PL), lipoprotein lipase (LPL), and monoglyceride lipase (MGL) enzymatic activities (n = 3). B: BAY (10−6 mol/l) inhibition of triglyceride hydrolase activity in Cos7 cells expressing human HSL or human ATGL (n = 3). C: Concentration-response inhibition by BAY of mouse adipose tissue extract triglyceride hydrolase activity (n = 3). D: BAY (10−6 mol/l) inhibition of triglyceride hydrolase activity in wild-type and HSL-null mouse adipose tissue extracts (n = 4). More
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<span class="search-highlight">Adipose</span> transcript levels and <span class="search-highlight">adipose</span> insulin resistance in fARKO mice. ...
Published: 13 April 2012
FIG. 4. Adipose transcript levels and adipose insulin resistance in fARKO mice. A: mRNA levels were measured for adipose triglyceride lipase (ATGL), fatty acid synthase (FAS), hormone-sensitive lipase (HSL), and lipoprotein lipase (LPL) by quantitative PCR in perigonadal and subcutaneous adipose tissue of 3-month-old fARKO and control mice on standard chow. B and C: Representative Western blots of IRS-1 pY612 phosphorylation in perigonadal and subcutaneous adipose tissue lysates. Data are mean ± SEM; (n = 8–10). *P < 0.05 control vs. fARKO, **P < 0.01 control vs. fARKO, ***P < 0.001 control vs. fARKO. con, control; ko, knockout; IP, immunoprecipitation; A.U., arbitrary unit. FIG. 4. Adipose transcript levels and adipose insulin resistance in fARKO mice. A: mRNA levels were measured for adipose triglyceride lipase (ATGL), fatty acid synthase (FAS), hormone-sensitive lipase (HSL), and lipoprotein lipase (LPL) by quantitative PCR in perigonadal and subcutaneous adipose tissue of 3-month-old fARKO and control mice on standard chow. B and C: Representative Western blots of IRS-1 pY612 phosphorylation in perigonadal and subcutaneous adipose tissue lysates. Data are mean ± SEM; (n = 8–10). *P < 0.05 control vs. fARKO, **P < 0.01 control vs. fARKO, ***P < 0.001 control vs. fARKO. con, control; ko, knockout; IP, immunoprecipitation; A.U., arbitrary unit. More
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<span class="search-highlight">Lipase</span> activities and gene expression in PKCεKO islets. <em>A</em>:...
Published: 28 April 2009
FIG. 6. Lipase activities and gene expression in PKCεKO islets. A: Lipase activities of islet homogenates after culture for 48 h in the presence of 0.4 mmol/l palmitate coupled to 0.92% BSA (Palm) or BSA alone (Cont) and subsequent stimulation with 20 mmol/l glucose for 1 h. Triglyceride or cholesteryl-ester lipid pools were provided as substrate, spiked with [3H]triolein or [14C]cholesteryl-oleate, respectively. Islets from n = 5 animals were used per genotype. B and C: mRNA expression of Hsl (Hsl), adipose triglyceride lipase (Atgl), adiponutrin (Adpn), α/β-hydrolase domain–containing protein 5 (Abhd5), translocator protein (Tspo), and lipoprotein lipase (Lpl). Islets were cultured in palmitate or control media, and data were expressed relative to wild-type control islets (n = 5). D: Western blot and quantification of Hsl and Atgl with 14-3-3β loading control; n = 3. □ = wild type, ■ = PKCεKO. Data are the means ± SE. *P < 0.05; **P < 0.01. WT, wild type. FIG. 6. Lipase activities and gene expression in PKCεKO islets. A: Lipase activities of islet homogenates after culture for 48 h in the presence of 0.4 mmol/l palmitate coupled to 0.92% BSA (Palm) or BSA alone (Cont) and subsequent stimulation with 20 mmol/l glucose for 1 h. Triglyceride or cholesteryl-ester lipid pools were provided as substrate, spiked with [3H]triolein or [14C]cholesteryl-oleate, respectively. Islets from n = 5 animals were used per genotype. B and C: mRNA expression of Hsl (Hsl), adipose triglyceride lipase (Atgl), adiponutrin (Adpn), α/β-hydrolase domain–containing protein 5 (Abhd5), translocator protein (Tspo), and lipoprotein lipase (Lpl). Islets were cultured in palmitate or control media, and data were expressed relative to wild-type control islets (n = 5). D: Western blot and quantification of Hsl and Atgl with 14-3-3β loading control; n = 3. □ = wild type, ■ = PKCεKO. Data are the means ± SE. *P < 0.05; **P < 0.01. WT, wild type. More
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Effects of salicylate on <span class="search-highlight">adipose</span> mRNA levels in mice. mRNA levels were meas...
Published: 14 March 2012
FIG. 3. Effects of salicylate on adipose mRNA levels in mice. mRNA levels were measured for AdiQ, TNF-α, MCP-1, lipoprotein lipase (LPL), adipose triglyceride lipase (ATGL), and angiotensinogen (AGT) by qPCR in omental (A), mesenteric (B), and subcutaneous (C) adipose tissue from C57Bl/6 DIO and HSD1KO-DIO mice. Data are mean ± SEM for n = 4–8 per group. Comparisons were by two-way ANOVA with Bonferroni post hoc tests. *P < 0.05, **P < 0.01 vs. vehicle of same genotype; фP < 0.05, ффP < 0.01, фффP < 0.001 vs. C57Bl/6 DIO vehicle. FIG. 3. Effects of salicylate on adipose mRNA levels in mice. mRNA levels were measured for AdiQ, TNF-α, MCP-1, lipoprotein lipase (LPL), adipose triglyceride lipase (ATGL), and angiotensinogen (AGT) by qPCR in omental (A), mesenteric (B), and subcutaneous (C) adipose tissue from C57Bl/6 DIO and HSD1KO-DIO mice. Data are mean ± SEM for n = 4–8 per group. Comparisons were by two-way ANOVA with Bonferroni post hoc tests. *P < 0.05, **P < 0.01 vs. vehicle of same genotype; фP < 0.05, ффP < 0.01, фффP < 0.001 vs. C57Bl/6 DIO vehicle. More
Journal Articles
Journal: Diabetes
Diabetes 2020;69(6):1178–1192
Published: 31 March 2020
... of lipolysis in T2D islets. Silencing adipose triglyceride lipase (ATGL) in human pseudoislets with shRNA targeting ATGL (shATGL) increased triglycerides (TGs) and the number and size of LDs, indicating that ATGL is the principal lipase in human β-cells. In shATGL pseudoislets, biphasic glucose-stimulated...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2011;60(6):1734–1742
Published: 21 May 2011
...). DAGs are by-products of lipolysis consecutive to TAG hydrolysis by adipose triglyceride lipase (ATGL) and are subsequently hydrolyzed by hormone-sensitive lipase (HSL). We hypothesized that an imbalance of ATGL relative to HSL (expression or activity) may contribute to DAG accumulation and insulin...
Includes: Supplementary data
Meeting Abstracts
Journal: Diabetes
Diabetes 2018;67(Supplement_1):154-OR
Published: 01 July 2018
... triacylglycerol (TAG) droplet accumulation (steatosis). The first step of TAG hydrolysis is catalyzed by adipose triglyceride lipase (ATGL), and its deficiency results in extreme cardiac steatosis in mice and humans. Although hormone-sensitive lipase (HSL) functions as a diacylglycerol (DAG) lipase in the heart...
Journal Articles
Journal: Diabetes
Diabetes 2011;60(5):1458–1466
Published: 23 April 2011
... of triacylglycerol metabolism in the liver that may act through adipose triglyceride lipase (ATGL). We used ATGL−/− mice to determine the role of PEDF in regulating lipid and glucose metabolism. RESEARCH DESIGN AND METHODS Recombinant PEDF was administered to ATGL−/− and wild-type mice...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2010;59(4):775–781
Published: 12 January 2010
... and metabolism remains largely unknown. RESEARCH DESIGN AND METHODS In this study, we analyzed the effect of activation and inhibition of the mammalian TORC1 (mTORC1) signaling pathway on the expression of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), lipolysis, lipogenesis, and lipid...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2015;64(3):733–745
Published: 05 November 2014
... and human adipocytes in vitro and in vivo in mice. PHD2 genetic ablation and pharmacological inhibition attenuated protein levels of the key lipolytic effectors hormone-sensitive lipase and adipose triglyceride lipase (ATGL), suggesting a link between adipocyte oxygen sensing and fatty acid release...
Includes: Supplementary data
Meeting Abstracts
Journal: Diabetes
Diabetes 2019;68(Supplement_1):290-OR
Published: 01 June 2019
... (VO2max) by ~10% (p=0.003), with no significant difference between HIIT and MICT. In muscle samples, hormone-sensitive lipase (HSL) protein abundance increased ~2-fold after training in HIIT (P<0.05), but not MICT. Training did not affect muscle abundance of adipose triglyceride lipase (ATGL) in either...
Journal Articles
Journal: Diabetes
Diabetes 2016;65(12):3561–3572
Published: 22 September 2016
...Mainak Ghosh; Sougata Niyogi; Madhumita Bhattacharyya; Moumita Adak; Dipak K. Nayak; Saikat Chakrabarti; Partha Chakrabarti Optimal control of hepatic lipid metabolism is critical for organismal metabolic fitness. In liver, adipose triglyceride lipase (ATGL) serves as a major triacylglycerol (TAG...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2016;65(5):1380–1386
Published: 16 February 2016
... protein levels of adipose triglyceride lipase (ATGL) nor phosphorylations of hormone-sensitive lipase were altered. The clinical picture of incipient DKA in adults can be reproduced by combined insulin deficiency and endotoxin-induced acute inflammation. The precipitating steps involve the release...
Includes: Supplementary data
Meeting Abstracts
Journal: Diabetes
Diabetes 2018;67(Supplement_1):2054-P
Published: 01 July 2018
... desaturase (SCD) mRNA, lipolysis from adipocyte triglyceride lipase (ATGL) and HSL mRNA by RTqPCR. The insulin resistant T2D had 63% and 22% higher HCL (p<0.01) and VAT (p<0.05) than CON. In SSAT, T2D featured 20%, 50%, 70%, 49% reductions in oxidative capacity, SCD, ATGL and HSL mRNA (all p<0.05...
Journal Articles
Journal: Diabetes
Diabetes 2017;66(5):1159–1171
Published: 01 March 2017
... hypertrophy in FKO mice likely resulted from compromised lipolytic activity as an outcome of decreased expression of adipose triglyceride lipase (ATGL), a key lipolytic enzyme. The suppression of ATGL in FKO mice was accounted for by the increased phosphorylation and acetylation of FoxO1, which compromises...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2008;57(8):2037–2045
Published: 01 August 2008
... in livers of diet-induced obese (DIO) mice were also measured. RESULTS— Cells lacking PAT proteins exhibited a dramatic increase in LD size and a decrease in LD number. Further, the lipolytic rate increased by ∼2- to 2.5-fold in association with increased adipose triglyceride lipase (ATGL) at the LD...
Includes: Supplementary data