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bhlh-basic-helix-loop-helix

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Hepatic de novo lipogenesis (DNL) is impaired in ChLO mice. <em>A</em>...
Published: 23 January 2020
Figure 1 Hepatic de novo lipogenesis (DNL) is impaired in ChLO mice. A: Schematic demonstration for the generation of ChREBP liver-specific knockout mice. B: Representative demonstration for PCR-based genotyping with tail genomic DNA. C: ChREBP deletion efficiency and specificity. ChREBP mRNA levels in liver, intestine, kidney, muscle, brown adipose tissue (BAT), and white adipose tissue (WAT). D: Western blot analysis for ChREBP protein expression in the liver. E: mRNA levels of glycolytic and lipogenic genes. F: Liver DNL was detected with the incorporation of 3H-H2O in vivo. *P < 0.05; **P < 0.01. bHLH, basic helix-loop-helix; DPM, disintegrations per minute; FA, fatty acid; NLS, nuclear localization signal; WT, wild type. Figure 1. Hepatic de novo lipogenesis (DNL) is impaired in ChLO mice. A: Schematic demonstration for the generation of ChREBP liver-specific knockout mice. B: Representative demonstration for PCR-based genotyping with tail genomic DNA. C: ChREBP deletion efficiency and specificity. ChREBP mRNA levels in liver, intestine, kidney, muscle, brown adipose tissue (BAT), and white adipose tissue (WAT). D: Western blot analysis for ChREBP protein expression in the liver. E: mRNA levels of glycolytic and lipogenic genes. F: Liver DNL was detected with the incorporation of 3H-H2O in vivo. *P < 0.05; **P < 0.01. bHLH, basic helix-loop-helix; DPM, disintegrations per minute; FA, fatty acid; NLS, nuclear localization signal; WT, wild type. More
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Regulators of <em>CCL2</em> in human adipocytes and obesity. <itali
Published: 17 July 2012
FIG. 3. Regulators of CCL2 in human adipocytes and obesity. A: The proposed CCL2 network depicting miRNAs (squares) as well as motifs and cognate TFs (circles) altered by obesity and potentially targeting CCL2 (diamond). T-bars represent inhibition; arrows represent stimulation. BD: miR-193b was overexpressed (white bar) or inhibited (black bar) in in vitro differentiated adipocytes, and mRNA levels of direct (MAX and ETS1) and indirect targets (RELB, STAT6, and NFKB1) (B) and CCL2 (C and D) were determined. To rule out unspecific effects, control cells were transfected with a negative control miRNA (striped bar). mRNA levels were measured 48 h after transfection. Results were analyzed using t test and are presented as fold change ± SEM relative to the negative control. ***P < 0.001, **P < 0.01, *P < 0.05. SP1, Sp1 transcription factor; NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; BHLH, basic helix-loop-helix; MYC, v-myc myelocytomatosis viral oncogene homolog; TFDP1, transcription factor Dp-1. (A high-quality color representation of this figure is available in the online issue.) FIG. 3. Regulators of CCL2 in human adipocytes and obesity. A: The proposed CCL2 network depicting miRNAs (squares) as well as motifs and cognate TFs (circles) altered by obesity and potentially targeting CCL2 (diamond). T-bars represent inhibition; arrows represent stimulation. B–D: miR-193b was overexpressed (white bar) or inhibited (black bar) in in vitro differentiated adipocytes, and mRNA levels of direct (MAX and ETS1) and indirect targets (RELB, STAT6, and NFKB1) (B) and CCL2 (C and D) were determined. To rule out unspecific effects, control cells were transfected with a negative control miRNA (striped bar). mRNA levels were measured 48 h after transfection. Results were analyzed using t test and are presented as fold change ± SEM relative to the negative control. ***P < 0.001, **P < 0.01, *P < 0.05. SP1, Sp1 transcription factor; NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; BHLH, basic helix-loop-helix; MYC, v-myc myelocytomatosis viral oncogene homolog; TFDP1, transcription factor Dp-1. (A high-quality color representation of this figure is available in the online issue.) More
Journal Articles
Journal: Diabetes
Diabetes 2001;50(3):694–696
Published: 01 March 2001
... and NeuroD1/BETA2, have been associated with diabetes. Cell type–specific members of the basic helix-loop-helix (bHLH) family of transcription factors play essential roles in the development and maintenance of many differentiated cell types, including pancreatic β-cells. Neurogenin 3 is a bHLH transcription...
Meeting Abstracts
Journal: Diabetes
Diabetes 2000;49(11):1955–1957
Published: 01 November 2000
...Y Horikawa; Y Horikawa; N J Cox; N Iwasaki; M Ogata; Y Iwamoto; V Schwitzgebel; M S German; G I Bell The basic helix-loop-helix (bHLH) family of transcription factors plays an important role in the normal development and function of the endocrine pancreas. Heterozygous mutations in the gene...
Images
<em>A</em>: Schematic representation of the PTF1A protein and locat...
Published: 09 June 2016
Figure 1 A: Schematic representation of the PTF1A protein and location of the mutations identified in all previously reported cases ( 1 – 3 ) and the p.P191T mutation found in the four current cases. B: Amino acid conservation in the bHLH domain. The p.P191T is the only missense mutation identified to date. It is located at a conserved residue within the basic helix-loop-helix (bHLH) domain (highlighted). ¥The p.Ala146_Arg154delinsGly mutation was previously reported as c.437_460del, p.Ala146_Arg154delfsX115 ( 3 ). Figure 1. A: Schematic representation of the PTF1A protein and location of the mutations identified in all previously reported cases (1–3) and the p.P191T mutation found in the four current cases. B: Amino acid conservation in the bHLH domain. The p.P191T is the only missense mutation identified to date. It is located at a conserved residue within the basic helix-loop-helix (bHLH) domain (highlighted). ¥The p.Ala146_Arg154delinsGly mutation was previously reported as c.437_460del, p.Ala146_Arg154delfsX115 (3). More
Journal Articles
Journal: Diabetes
Diabetes 2001;50(5):928–936
Published: 01 May 2001
...Jane C. Lee; Stewart B. Smith; Hirotaka Watada; Joseph Lin; David Scheel; Juehu Wang; Raghavendra G. Mirmira; Michael S. German Neurogenin3 (ngn3), a basic helix-loop-helix (bHLH) transcription factor, functions as a pro-endocrine factor in the developing pancreas: by itself, it is sufficient...
Meeting Abstracts
Journal: Diabetes
Diabetes 2000;49(2):163–176
Published: 01 February 2000
...+ epithelial cells that are marked by expression of Neurogenin3 (Ngn3). Ngn3, a member of the basic helix-loop-helix (bHLH) family of transcription factors, is suggested to act upstream of NeuroD in a bHLH cascade. Detailed analysis of Ngn3/paired box factor 6 (PAX6) and NeuroD/PAX6 co-expression shows...
Meeting Abstracts
Journal: Diabetes
Diabetes 1999;48(2):416–419
Published: 01 February 1999
... the same gene as the BETA2 ( -cell E-box results in the substitution of alanine to threonine at codon transactivator 2), a class B basic helix-loop-helix (bHLH) 45 (Ala45Thr). The frequencies of heterozygotes for the protein expressed in pancreatic endocrine cells, intestine, Ala45Thr variant were 9.8...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(5):1179–1189
Published: 01 May 2006
...Ming V. Li; Benny Chang; Minako Imamura; Naravat Poungvarin; Lawrence Chan We report here a novel mechanism for glucose-mediated activation of carbohydrate response element binding protein (ChREBP), a basic helix-loop-helix/leucine zipper (bHLH/ZIP) transcription factor of Mondo family that binds...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 1997;46(3):354–362
Published: 01 March 1997
... that bind to the C/EBP binding site regulate it negatively in CHO cells. Address correspondence and reprint requests to Eiichi Araki, Department of Metabolic Medicine, Kumamoto University School of Medicine, 1-1-1, Honjo, Kumamoto, 860, Japan. 1 bHLH, basic helix-loop-helix; CAT, chloramphenicol...
Journal Articles
Journal: Diabetes
Diabetes 2003;52(2):403–408
Published: 01 February 2003
... is also necessary for stimulation in β-cells and enteroendocrine cells. Gel shift and transfection experiments suggested that regulation of both the βGK and insulin E-box was mediated by the same set of proteins, an activator consisting of basic helix-loop-helix (bHLH) proteins that are islet and neuronal...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(10):2859–2866
Published: 01 October 2005
..., Japan. E-mail: noguchih@kuhp.kyoto-u.ac.jp 20 7 2005 19 5 2005 DIABETES 2005 bHLH, basic helix-loop-helix dNeuroD, deletion BETA2/NeuroD dNeuroD-6, PTD-deleted BETA2/NeuroD protein DsRed, Discosoma sp. red fluorescent protein DynDN, dominant-negative mutant...
Meeting Abstracts
Journal: Diabetes
Diabetes 1998;47(12):1817–1823
Published: 01 December 1998
... rapidly become one of the most studied NeuroD/Beta2. NeuroD/Beta2 is a cell-type restricted basic organs with respect to function of transcription factors, and helix-loop-helix (bHLH) transcription factor that is expressed many pancreatic phenotypes have been described. However, in all pancreatic...
Meeting Abstracts
Journal: Diabetes
Diabetes 2001;50(suppl_1):S5
Published: 01 February 2001
... developmental program. classic embryological experiments describing the morpho- genesis of the pancreas and the epithelio-mesenchymal Several homeodomain and basic helix-loop-helix (bHLH) interactions that are instrumental for proper pancreas transcription factors, notably Isl1, Nkx2.2, Pax4, Pax6...
Meeting Abstracts
Journal: Diabetes
Diabetes 1998;47(9):1451–1458
Published: 01 September 1998
... mice were homogenized in Ultraspec RNA (Biotecx, Hous- Deceased 1995. ton, TX) (50 mg/ml) on ice for 30 sec at maximum speed using a Polytron homog- bHLH, basic helix-loop-helix protein; dNTP, deoxynucleotide triphos- enizer (Kinematika, Switzerland). Next 0.2 ml of chloroform was added per ml...
Journal Articles
Journal: Diabetes
Diabetes 2012;61(8):1986–1993
Published: 17 July 2012
... to the negative control. ***P < 0.001, **P < 0.01, *P < 0.05. SP1, Sp1 transcription factor; NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; BHLH, basic helix-loop-helix; MYC, v-myc myelocytomatosis viral oncogene homolog; TFDP1, transcription...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2016;65(9):2810–2815
Published: 09 June 2016
... mutation identified to date. It is located at a conserved residue within the basic helix-loop-helix (bHLH) domain (highlighted). ¥The p.Ala146_Arg154delinsGly mutation was previously reported as c.437_460del, p.Ala146_Arg154delfsX115 ( 3 ). Figure 1. A: Schematic representation of the PTF1A protein...
Journal Articles
Journal: Diabetes
Diabetes 1996;45(11):1478–1488
Published: 01 November 1996
...@medone.med.osaka-u.ac.jp. identified in some pedigrees of maturity-onset diabetes of the young as well as in ordinary NIDDM patients Received for publication 7 February 1996 and accepted in revised form (18,19). Defects in the GK gene seemed to be involved in 27 June 1996. bHLH, basic helix-loop-helix; GK...
Journal Articles
Journal: Diabetes
Diabetes 2020;69(4):591–602
Published: 23 January 2020
... with the incorporation of 3H-H2O in vivo. *P < 0.05; **P < 0.01. bHLH, basic helix-loop-helix; DPM, disintegrations per minute; FA, fatty acid; NLS, nuclear localization signal; WT, wild type. Figure 1. Hepatic de novo lipogenesis (DNL) is impaired in ChLO mice...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2007;56(4):921–929
Published: 01 April 2007
... correspondence and reprint requests to H. Teresa Ku, Box 1496, Mount Sinai School of Medicine, New York, NY 10029-6574. E-mail: hsun.ku@mssm.edu 22 12 2006 10 4 2006 DIABETES 2007 bHLH, basic helix-loop-helix CM, conditioned media EGFP, enhanced green fluorescent protein E, embryonic...
Includes: Supplementary data