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cfse-carboxyfluorescein-diacetate-succinimidyl-ester

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Journal Articles
Journal: Diabetes
Diabetes 2009;58(8):1789–1796
Published: 28 April 2009
...+ T-cells to WPs, ovalbumin, gliadin, α-gliadin 33-mer peptide, tetanus toxoid, and phytohemagglutinin was measured using a carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay. T–helper cell type 1 (Th1), Th2, and Th17 cytokines were analyzed in WP-stimulated peripheral blood...
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In vitro functional capacity of CD4<sup>+</sup>CD25<sup>+</sup> T-cells. ...
Published: 01 May 2005
FIG. 3. In vitro functional capacity of CD4+CD25+ T-cells. A: We used 6-week-old (n = 22), 8-week-old (n = 5), and diabetic NOD (n = 17) mice. Cocultures were performed by incubating CD4+CD25+ and CD4+CD25 T-cells from the same mouse at a 1:1 ratio with APCs and anti-CD3 antibody. A significant difference was observed when comparing mean values between 6-week-old and diabetic NOD mice (P < 0.0001) and between 6- and 8-week-old NOD mice (P < 0.042). The addition of anti–TGF-β antibody, 2G.7, reversed the suppression in a dose-dependent manner (no antibody: 60.9 ± 2.6% inhibition; + 2G.7 10 μg/ml: 32.8 ± 3.6% inhibition, P < 0.005; + 2G.7 50 μg/ml: 4.5 ± 1.2% inhibition, P < 0.001). B: Suppression of CD4+CD25 T-cell proliferation by increasing proportion of CD4+CD25+ T-cells. C: In vitro inhibition of CD4+CD25 T-cells by CD4+CD25+ T-cells is cell contact–dependent. The proliferation of 5,6-carboxyfluorescein diacetate-succinimidyl ester (CFSE)-labeled CD4+CD25 T-cells from 6-week-old NOD mice was analyzed by immunofluorescence after being incubated 4 days with young NOD CD4+CD25+ T-cells separated or not separated by a transwell membrane. The results were representative of 5–8 experiments. wk, week. FIG. 3. In vitro functional capacity of CD4+CD25+ T-cells. A: We used 6-week-old (n = 22), 8-week-old (n = 5), and diabetic NOD (n = 17) mice. Cocultures were performed by incubating CD4+CD25+ and CD4+CD25− T-cells from the same mouse at a 1:1 ratio with APCs and anti-CD3 antibody. A significant difference was observed when comparing mean values between 6-week-old and diabetic NOD mice (P < 0.0001) and between 6- and 8-week-old NOD mice (P < 0.042). The addition of anti–TGF-β antibody, 2G.7, reversed the suppression in a dose-dependent manner (no antibody: 60.9 ± 2.6% inhibition; + 2G.7 10 μg/ml: 32.8 ± 3.6% inhibition, P < 0.005; + 2G.7 50 μg/ml: 4.5 ± 1.2% inhibition, P < 0.001). B: Suppression of CD4+CD25− T-cell proliferation by increasing proportion of CD4+CD25+ T-cells. C: In vitro inhibition of CD4+CD25− T-cells by CD4+CD25+ T-cells is cell contact–dependent. The proliferation of 5,6-carboxyfluorescein diacetate-succinimidyl ester (CFSE)-labeled CD4+CD25− T-cells from 6-week-old NOD mice was analyzed by immunofluorescence after being incubated 4 days with young NOD CD4+CD25+ T-cells separated or not separated by a transwell membrane. The results were representative of 5–8 experiments. wk, week. More
Journal Articles
Journal: Diabetes
Diabetes 2007;56(8):2116–2123
Published: 01 August 2007
... Medicine, University of Florida, Gainesville, FL 32610. E-mail: xia@pathology.ufl.edu 8 5 2007 16 6 2006 DIABETES 2007 APC, allophycocyanin CFSE, carboxyfluorescein diacetate succinimidyl ester IFN, interferon IL, interleukin MFI, mean fluorescence intensity PLN, pancreatic...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2005;54(7):2019–2025
Published: 01 July 2005
... labeled with 5-(and −6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Eugene, OR) as previously described ( 20 ). Briefly, splenocytes were resuspended at a concentration of 10 × 106 cells/ml in serum-free IMDM (Gibco/BRL, Gaithersburg, MD) at 37°C. An equal volume...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(4):971–977
Published: 01 April 2004
... cell CFSE, carboxyfluorescein diacetate succinimidyl ester IFN, interferon IL, interleukin mAb, monoclonal antibody MBP, myelin basic protein MHC, major histocompatibility complex PBMC, peripheral blood mononuclear cell PE, phycoerythrin PLC, phospholipase C sFasL, soluble Fas ligand TCR, T...
Journal Articles
Journal: Diabetes
Diabetes 1992;41(12):1603–1608
Published: 01 December 1992
..., insulin-dependent diabetes mellitus; STZ, strepto- measured on whole blood samples with an Exactech pen zocin; Cl, confidence interval; IL-2, interleukin-2; HBSS, Hank's balanced salt and strips (MediSense, Cambridge, MA). The normal solution; CFSE, carboxyfluorescein diacetate succinimidyl ester; PBS...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(6):1686–1693
Published: 01 June 2007
... cell CFSE, carboxyfluorescein diacetate succinimidyl ester DC, dendritic cell EAE, experimental autoimmune encephalomyelitis hCG, human chorionic gonadotropin IDO, indoleamine 2,3-dioxygenase IFN-γ, γ-interferon IL, interleukin MHC, major histocompatibility complex Type 1 diabetes is a T...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(10):2551–2560
Published: 01 October 2007
... with 0.5 or 5 μmol/l carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Carlsbad, CA) or with 10 μmol/l CellTracker Orange (Molecular Probes) for 10 min at 37°C. Labeling was stopped by quenching with complete Dulbecco's modified Eagle's medium on ice for 5 min. After washing, cells...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(6):1571–1580
Published: 01 June 2006
... DIABETES 2006 CFSE, carboxyfluorescein diacetate succinimidyl ester FACS, fluorescence-activated cell sorting IFN-γ, γ-interferon ICAM, intracellular adhesion molecule IL, interleukin mAb, monoclonal antibody TGF, transforming growth factor Tr, T regulatory Immunosuppressive therapy...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(2):306–310
Published: 01 February 2005
...-mail: jaeckel_elmar@yahoo.com 24 11 2004 11 10 2004 DIABETES 2005 CFSE, 5,6-carboxyfluorescein diacetate-succinimidyl ester eGFP, enhanced green fluorescent protein IL, interleukin Recessive tolerance toward a single autoantigen was so far unable to completely prevent...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2006;55(1):40–49
Published: 01 January 2006
... 5 10 2005 13 5 2005 DIABETES 2006 CFSE, carboxyfluorescein diacetate succinimidyl ester FACS, fluorescence-activated cell sorting FoxP3, transcription factor Forkhead box P3 GvHD, graft versus host disease IL, interleukin mAb, monoclonal antibody OVA, ovalbumin peptide 323...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(11):2797–2803
Published: 01 November 2004
... suspensions were prepared from pooled spleens of BDC2.5/NOD mice depleted of erythrocytes in 0.83% NH4Cl, adjusted to 107/ml in PBS, and incubated for 10 min at 37°C in the presence of 2.5 mmol/l 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE; Molecular Probes) prepared as a 5...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(5):1415–1422
Published: 01 May 2005
.... The proliferation of 5,6-carboxyfluorescein diacetate-succinimidyl ester (CFSE)-labeled CD4+CD25− T-cells from 6-week-old NOD mice was analyzed by immunofluorescence after being incubated 4 days with young NOD CD4+CD25+ T-cells separated or not separated by a transwell membrane. The results were representative of 5...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(2):434–442
Published: 01 February 2005
.... Irene Caminschi, Walter and Eliza Hall Institute. 5(6)-Carboxyfluorescein diacetate succinimidyl ester (CFSE) was purchased from Molecular Probes (Eugene, OR). A small proportion (8.5 ± 2.1% [mean ± SD], n = 20) of GM-CSF/TGF-β1 BM cells had a monocyte-like or immature DC-like appearance...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2006;55(4):1004–1010
Published: 01 April 2006
... with CellQuest software (Becton Dickinson). To measure T-cell proliferation, 2 × 106 splenocytes were labeled with 2 μmol/l carboxyfluorescein diacetate succinimidyl ester (Molecular Probes, Leiden, the Netherlands) and cultured with either the mimotope of the dominant cytotoxic T-lymphocyte...
Journal Articles
Journal: Diabetes
Diabetes 2014;63(10):3458–3469
Published: 15 September 2014
... and proliferation analyzed by BrdU (A) and carboxyfluorescein succinimidyl ester (CFSE) (B) assays. T cells were first treated with different concentrations of Cpd K for 24 h and then with 0, 50, and 100 units/mL exogenous IL-2 for another 48 h. C: BrdU was used to analyze T-cell anergy...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(8):2003–2011
Published: 01 August 2004
...-) carboxyfluorescein diacetate succinimidyl ester FasL, Fas ligand FITC, fluorescein isothiocyanate IAA, insulin autoantibody IL, interleukin MHC, major histocompatibility complex PE, phycoerythrin PLN, pancreatic lymph node sIgM, surface IgM TCR, T-cell receptor Autoimmune type 1 diabetes...
Journal Articles
Journal: Diabetes
Diabetes 2014;63(1):68–74
Published: 13 December 2013
.... Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled CD4+CD25- cells (effectors) were cultured with graded numbers of CD4+CD25+ (Tregs) for 4 days. In all culture conditions, CD137-deficient Tregs showed a trend of less suppressive than the wild-type control...
Journal Articles
Journal: Diabetes
Diabetes 2012;61(11):2881–2892
Published: 16 October 2012
... that TGF-β has an important role in the regulatory phenotype of macrophages to suppress T-cell activation mediated both by cell contact and via secreted factors. NOD splenocytes were also stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) and cocultured with induced M2r macrophages...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2013;62(8):2762–2772
Published: 17 July 2013
... and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester . Nat Protoc   2007 ; 2 : 2049 – 2056 17853860 42. Moro   K , Yamada   T , Tanabe   M , et al . Innate production of T(H)2 cytokines by adipose tissue-associated c-Kit(+)Sca-1(+) lymphoid...
Includes: Supplementary data