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cfse-carboxyfluorescein-diacetate-succinimidyl-ester

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Journal Articles
Journal: Diabetes
Diabetes 2009;58(8):1789–1796
Published: 28 April 2009
...+ T-cells to WPs, ovalbumin, gliadin, α-gliadin 33-mer peptide, tetanus toxoid, and phytohemagglutinin was measured using a carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay. T–helper cell type 1 (Th1), Th2, and Th17 cytokines were analyzed in WP-stimulated peripheral blood...
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In vitro functional capacity of CD4<sup>+</sup>CD25<sup>+</sup> T-cells. ...
Published: 01 May 2005
FIG. 3. In vitro functional capacity of CD4+CD25+ T-cells. A: We used 6-week-old (n = 22), 8-week-old (n = 5), and diabetic NOD (n = 17) mice. Cocultures were performed by incubating CD4+CD25+ and CD4+CD25 T-cells from the same mouse at a 1:1 ratio with APCs and anti-CD3 antibody. A significant difference was observed when comparing mean values between 6-week-old and diabetic NOD mice (P < 0.0001) and between 6- and 8-week-old NOD mice (P < 0.042). The addition of anti–TGF-β antibody, 2G.7, reversed the suppression in a dose-dependent manner (no antibody: 60.9 ± 2.6% inhibition; + 2G.7 10 μg/ml: 32.8 ± 3.6% inhibition, P < 0.005; + 2G.7 50 μg/ml: 4.5 ± 1.2% inhibition, P < 0.001). B: Suppression of CD4+CD25 T-cell proliferation by increasing proportion of CD4+CD25+ T-cells. C: In vitro inhibition of CD4+CD25 T-cells by CD4+CD25+ T-cells is cell contact–dependent. The proliferation of 5,6-carboxyfluorescein diacetate-succinimidyl ester (CFSE)-labeled CD4+CD25 T-cells from 6-week-old NOD mice was analyzed by immunofluorescence after being incubated 4 days with young NOD CD4+CD25+ T-cells separated or not separated by a transwell membrane. The results were representative of 5–8 experiments. wk, week. FIG. 3. In vitro functional capacity of CD4+CD25+ T-cells. A: We used 6-week-old (n = 22), 8-week-old (n = 5), and diabetic NOD (n = 17) mice. Cocultures were performed by incubating CD4+CD25+ and CD4+CD25− T-cells from the same mouse at a 1:1 ratio with APCs and anti-CD3 antibody. A significant difference was observed when comparing mean values between 6-week-old and diabetic NOD mice (P < 0.0001) and between 6- and 8-week-old NOD mice (P < 0.042). The addition of anti–TGF-β antibody, 2G.7, reversed the suppression in a dose-dependent manner (no antibody: 60.9 ± 2.6% inhibition; + 2G.7 10 μg/ml: 32.8 ± 3.6% inhibition, P < 0.005; + 2G.7 50 μg/ml: 4.5 ± 1.2% inhibition, P < 0.001). B: Suppression of CD4+CD25− T-cell proliferation by increasing proportion of CD4+CD25+ T-cells. C: In vitro inhibition of CD4+CD25− T-cells by CD4+CD25+ T-cells is cell contact–dependent. The proliferation of 5,6-carboxyfluorescein diacetate-succinimidyl ester (CFSE)-labeled CD4+CD25− T-cells from 6-week-old NOD mice was analyzed by immunofluorescence after being incubated 4 days with young NOD CD4+CD25+ T-cells separated or not separated by a transwell membrane. The results were representative of 5–8 experiments. wk, week. More
Journal Articles
Journal: Diabetes
Diabetes 2007;56(8):2116–2123
Published: 01 August 2007
... induction by using β-cell antigens. APC, allophycocyanin CFSE, carboxyfluorescein diacetate succinimidyl ester IFN, interferon IL, interleukin MFI, mean fluorescence intensity PLN, pancreatic draining lymph node TCR, T-cell receptor Th, T-helper Tr1, T-regulatory type 1 UVB, ultraviolet B...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2005;54(7):2019–2025
Published: 01 July 2005
...) using CellQuest software. Splenocytes were labeled with 5-(and −6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Eugene, OR) as previously described ( 20 ). Briefly, splenocytes were resuspended at a concentration of 10 × 106 cells/ml in serum-free IMDM...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(4):971–977
Published: 01 April 2004
... at Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101-2795. E-mail: nepom@benaroyaresearch.org 8 1 2004 27 8 2003 DIABETES 2004 AICD, activation-induced cell death ann-V, annexin V APC, antigen-presenting cell CFSE, carboxyfluorescein diacetate succinimidyl ester IFN, interferon...
Journal Articles
Journal: Diabetes
Diabetes 1992;41(12):1603–1608
Published: 01 December 1992
..., insulin-dependent diabetes mellitus; STZ, strepto- measured on whole blood samples with an Exactech pen zocin; Cl, confidence interval; IL-2, interleukin-2; HBSS, Hank's balanced salt and strips (MediSense, Cambridge, MA). The normal solution; CFSE, carboxyfluorescein diacetate succinimidyl ester; PBS...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(6):1686–1693
Published: 01 June 2007
... cell CFSE, carboxyfluorescein diacetate succinimidyl ester DC, dendritic cell EAE, experimental autoimmune encephalomyelitis hCG, human chorionic gonadotropin IDO, indoleamine 2,3-dioxygenase IFN-γ, γ-interferon IL, interleukin MHC, major histocompatibility complex Type 1 diabetes is a T...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(10):2551–2560
Published: 01 October 2007
... with 0.5 or 5 μmol/l carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Carlsbad, CA) or with 10 μmol/l CellTracker Orange (Molecular Probes) for 10 min at 37°C. Labeling was stopped by quenching with complete Dulbecco's modified Eagle's medium on ice for 5 min. After washing, cells...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(6):1571–1580
Published: 01 June 2006
.... Diabetes development was monitored by glucose levels. Splenic T-cells of diabetic NOD mice were purified by anti-CD90 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and were stained with carboxyfluorescein diacetate succinimidyl ester (CFSE; Molecular Probes, Eugene, OR), as previously...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(2):306–310
Published: 01 February 2005
... and Endocrinology, Carl Neuberg Str. 1, 30625 Hannover, Germany. E-mail: jaeckel_elmar@yahoo.com 24 11 2004 11 10 2004 DIABETES 2005 CFSE, 5,6-carboxyfluorescein diacetate-succinimidyl ester eGFP, enhanced green fluorescent protein IL, interleukin Recessive tolerance toward a single...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2006;55(1):40–49
Published: 01 January 2006
... 5 10 2005 13 5 2005 DIABETES 2006 CFSE, carboxyfluorescein diacetate succinimidyl ester FACS, fluorescence-activated cell sorting FoxP3, transcription factor Forkhead box P3 GvHD, graft versus host disease IL, interleukin mAb, monoclonal antibody OVA, ovalbumin peptide 323...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(11):2797–2803
Published: 01 November 2004
..., adjusted to 107/ml in PBS, and incubated for 10 min at 37°C in the presence of 2.5 mmol/l 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE; Molecular Probes) prepared as a 5 mmol/l stock solution in DMSO. After being incubated, the cells were washed twice with RPMI supplemented with 5...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(5):1415–1422
Published: 01 May 2005
.... The proliferation of 5,6-carboxyfluorescein diacetate-succinimidyl ester (CFSE)-labeled CD4+CD25− T-cells from 6-week-old NOD mice was analyzed by immunofluorescence after being incubated 4 days with young NOD CD4+CD25+ T-cells separated or not separated by a transwell membrane. The results were representative of 5...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(2):434–442
Published: 01 February 2005
... Parade Parkville, 3050 Victoria, Australia. E-mail: harrison@wehi.edu.au 11 11 2004 4 8 2004 DIABETES 2005 BM, bone marrow CFSE, 5(6)-carboxyfluorescein diacetate succinimidyl ester DC, dendritic cell FITC, fluorescein isothiocyanate GM-CSF, granulocyte macrophage colony...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2006;55(4):1004–1010
Published: 01 April 2006
... with CellQuest software (Becton Dickinson). To measure T-cell proliferation, 2 × 106 splenocytes were labeled with 2 μmol/l carboxyfluorescein diacetate succinimidyl ester (Molecular Probes, Leiden, the Netherlands) and cultured with either the mimotope of the dominant cytotoxic T-lymphocyte...
Journal Articles
Journal: Diabetes
Diabetes 2014;63(10):3458–3469
Published: 15 September 2014
... and proliferation analyzed by BrdU (A) and carboxyfluorescein succinimidyl ester (CFSE) (B) assays. T cells were first treated with different concentrations of Cpd K for 24 h and then with 0, 50, and 100 units/mL exogenous IL-2 for another 48 h. C: BrdU was used to analyze T-cell anergy...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(8):2003–2011
Published: 01 August 2004
... N6G 2V4, Canada. E-mail: del@robarts.ca 20 5 2004 26 2 2004 DIABETES 2004 AICD, activation-induced cell death APC, antigen-presenting cell BCR, B-cell receptor CFSE, 5- (and 6-) carboxyfluorescein diacetate succinimidyl ester FasL, Fas ligand FITC, fluorescein...
Journal Articles
Journal: Diabetes
Diabetes 2014;63(1):68–74
Published: 13 December 2013
.... Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled CD4+CD25- cells (effectors) were cultured with graded numbers of CD4+CD25+ (Tregs) for 4 days. In all culture conditions, CD137-deficient Tregs showed a trend of less suppressive than the wild-type control...
Journal Articles
Journal: Diabetes
Diabetes 2012;61(11):2881–2892
Published: 16 October 2012
... together, these results indicate that TGF-β has an important role in the regulatory phenotype of macrophages to suppress T-cell activation mediated both by cell contact and via secreted factors. NOD splenocytes were also stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) and cocultured...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2013;62(8):2762–2772
Published: 17 July 2013
... 10861034 41. Quah   BJ , Warren   HS , Parish   CR . Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester . Nat Protoc   2007 ; 2 : 2049 – 2056 17853860 42. Moro   K , Yamada   T...
Includes: Supplementary data