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gkrp-gk-regulatory-protein

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Journal Articles
Journal: Diabetes
Diabetes 2004;53(3):535–541
Published: 01 March 2004
... acid(GKA2), which increase the affinity of GK for glucose by 4- and 11-fold, respectively. GKA1 increased the affinity of GK for the competitive inhibitor mannoheptulose but did not affect the affinity for the inhibitors palmitoyl-CoA and the endogenous 68-kDa regulator (GK regulatory protein [GKRP...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(7):1773–1782
Published: 01 July 2007
... immunoreactivity–to–GK mRNA ratio than wild-type GFP GK. Heterologous expression of liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK2/FDP2) in cell lines increased GK activity for wild-type GK and V62M but not for G72R, whereas expression of liver GK regulatory protein (GKRP) increased GK activity...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(9):2479–2490
Published: 01 September 2006
...) and its inhibitory protein, GK regulatory protein (GKRP), were colocalized in the cytoplasm of hepatocytes. At day 7 of drug administration, EGP was slightly reduced, but G6Pase flux and GC were markedly lower compared with the nontreated group. In this case, GK and GKRP were colocalized in the nuclei...
Journal Articles
Journal: Diabetes
Diabetes 2001;50(8):1813–1820
Published: 01 August 2001
...Eric D. Slosberg; Urvi J. Desai; Barbara Fanelli; Irene St. Denny; Sheila Connelly; Michael Kaleko; Brian R. Boettcher; Shari L. Caplan The enzyme glucokinase (GK) plays a central role in glucose homeostasis. Hepatic GK activity is acutely controlled by the action of the GK regulatory protein (GKRP...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(suppl_1):S66–S70
Published: 01 February 2004
... that peroxisome proliferator-activated receptor gamma activation has coordinate effects on gene expression in multiple insulin-sensitive tissues. Endocrinology   142 : 1269 –1277, 2001 EMSA, electrophoretic mobility shift assay GK, glucokinase GKRP, GK regulatory protein LGK, live type glucokinase...
Journal Articles
Journal: Diabetes
Diabetes 2001;50(3):622–629
Published: 01 March 2001
... Immunoresearch) for 1 h at room temperature. The same blot was washed and incubated first with a rabbit anti–GK regulatory protein (GKRP) serum (diluted 1:5,000), then with a horseradish peroxidase–conjugated donkey anti-rabbit IgG. A solution consisting of 1.25 mmol/l luminol, 0.2 mmol/l para-coumaric acid...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(4):893–900
Published: 01 April 2002
... demonstrated GK mRNA in other locations that have been implicated in glucose sensing, such as the paraventricular and ventro-medial hypothalamic (VMH) nuclei in the brain ( 15 , 16 ). GK activity is acutely regulated by its interaction with GK regulatory protein (GKRP). The regulatory protein located...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(suppl_3):S394–S404
Published: 01 December 2002
... glucose phosphorylation rate; F1P, fructose-1-phosphate; F6P, fructose-6-phosphate; G6P, glucose-6-phosphate; GK, glucokinase; GKB, GK content of the β-cell; GK-L, liver isoform of GK; GKRP, GK regulatory protein; GSIR, glucose-stimulated insulin release; PHHI-GK; GK-linked persistent hyperinsulinemic...
Journal Articles
Journal: Diabetes
Diabetes 1997;46(2):179–186
Published: 01 February 1997
..., fructose-6-phosphate; FBS, fetal bovine serum; GK, glucokinase; GKRP, glucokinase regulatory protein; IBMX, isobutylmethylxanthine; KH, Krebs-Henseleit buffer; L-PK, liver type pyruvate kinase; MODY, maturity onset diabetes of the young; PBS, phosphate-buffered saline; SCD1, stearoyl CoA desaturase I...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(3):549–559
Published: 01 March 2004
...-aminobutyric acid GE, glucose excited GI, glucose inhibited GK, glucokinase GKRP, GK regulatory protein HK-I, hexokinase-I INS-R, insulin receptor KATP channel, ATP-sensitive K+ channel Kir, pore-forming, inwardly rectifying K+ channel LDH, lactate dehydrogenase Lepr...
Journal Articles
Journal: Diabetes
Diabetes 2017;66(5):1172–1184
Published: 28 February 2017
... in plasma glucose and in glycogen glucose were determined by selective enzymatic detritiation of [2-3H]-glucose ( 18 , 22 ). Western blotting was performed to quantify the levels of glucokinase (GK), GK regulatory protein (GKRP), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2009;58(1):78–86
Published: 01 January 2009
... of diabetes development in ZDF rats, is associated with impaired regulation of GK by GK regulatory protein (GKRP) ( 15 – 17 ). In our current study, we investigated GK expression in liver during the progressive development of diabetes in ZDF rats and examined what correlation altered GK expression may have...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2013;62(2):392–400
Published: 17 January 2013
... University School of Medicine, Molecular Physiology and Biophysics Department). All other antibodies were purchased from Santa Cruz Biotechnology (actin and GK regulatory protein [GKRP]) or Cell Signaling Technology (GS). Test protein bands were quantified using ImageJ software (National Institutes of Health...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2011;60(12):3175–3185
Published: 13 November 2011
... tool to investigate these mechanisms ( 13 ). Regulation of GK at the posttranslational level differs in the liver and pancreatic β-cells ( 14 ). In hepatocytes, GK is regulated by binding to the liver regulatory protein (GKRP) ( 15 ), which modulates GK activity by sequestration of inactive GK...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2009;58(1):18–20
Published: 01 January 2009
... in newly diagnosed type 2 diabetic patients ( 8 ) or decreased in obese subjects with diabetes ( 9 ). Hepatic glucokinase is regulated by an inhibitory protein (glucokinase regulatory protein [GKRP]) that binds glucokinase with high affinity at basal glucose concentrations (5 mmol/l) and sequesters...
Journal Articles
Journal: Diabetes
Diabetes 2003;52(6):1326–1332
Published: 01 June 2003
... mRNA are 30 h and 45 min respectively [ 30 , 31 ].) During fasting, GK is tightly bound to the inhibitory GK regulatory protein (GKRP) ( 32 ) in the nucleus ( 33 ) (except perivenously [ 13 ]). Farrelly et al. ( 34 ) and Grimsby et al. ( 35 ) demonstrated that knockout of the GKRP in mice resulted...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(9):2346–2352
Published: 01 September 2004
..., The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, U.K. E-mail: loranne.agius@ncl.ac.uk 10 5 2004 8 1 2004 DIABETES 2004 FBS, fetal bovine serum GAPDH, glyceraldehyde-3-phosphate dehydrogenase GKRP, glucokinase regulatory protein PFK-2, 6...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(1):1–12
Published: 01 January 2006
... MD, University of Pennsylvania, Department of BiochemistryBiophysics, 501 Stemmler Hall, Philadelphia, PA 19104. E-mail: matsch@mail.med.upenn.edu 23 8 2005 20 7 2005 DIABETES 2006 GKA, glucokinase activator drug GKRP, glucokinase regulatory protein GSIR, glucose...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(6):1713–1722
Published: 01 June 2006
... the stability of G72R and G264S differed only slightly from that of wild type. G72R and M210K did not respond to an allosteric glucokinase activator (GKA) or the hepatic glucokinase regulatory protein (GKRP). Mutation analysis of the role of glycine at position 72 by substituting E, F, K, M, S, or Q showed...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(4):865–874
Published: 01 April 2006
...-P2, fructose 2,6-bisphosphate G6Pase, glucose-6-phosphatase GKRP, glucokinase regulatory protein Glc-6-P, glucose 6-phosphate HBM, hepatocyte basal medium PFK, 6-phosphofructokinase PKA, protein kinase A PKB, protein kinase B The control of glucose uptake depends on the relative rates...
Includes: Supplementary data