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gkrp-gk-regulatory-protein

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Journal Articles
Journal: Diabetes
Diabetes 2004;53(3):535–541
Published: 01 March 2004
... acid(GKA2), which increase the affinity of GK for glucose by 4- and 11-fold, respectively. GKA1 increased the affinity of GK for the competitive inhibitor mannoheptulose but did not affect the affinity for the inhibitors palmitoyl-CoA and the endogenous 68-kDa regulator (GK regulatory protein [GKRP...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(7):1773–1782
Published: 01 July 2007
... immunoreactivity–to–GK mRNA ratio than wild-type GFP GK. Heterologous expression of liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK2/FDP2) in cell lines increased GK activity for wild-type GK and V62M but not for G72R, whereas expression of liver GK regulatory protein (GKRP) increased GK activity...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(9):2479–2490
Published: 01 September 2006
...) and its inhibitory protein, GK regulatory protein (GKRP), were colocalized in the cytoplasm of hepatocytes. At day 7 of drug administration, EGP was slightly reduced, but G6Pase flux and GC were markedly lower compared with the nontreated group. In this case, GK and GKRP were colocalized in the nuclei...
Journal Articles
Journal: Diabetes
Diabetes 2001;50(8):1813–1820
Published: 01 August 2001
...Eric D. Slosberg; Urvi J. Desai; Barbara Fanelli; Irene St. Denny; Sheila Connelly; Michael Kaleko; Brian R. Boettcher; Shari L. Caplan The enzyme glucokinase (GK) plays a central role in glucose homeostasis. Hepatic GK activity is acutely controlled by the action of the GK regulatory protein (GKRP...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(suppl_1):S66–S70
Published: 01 February 2004
...-mail: yha111@yumc.yonsei.ac.kr 2 6 2003 17 3 2003 DIABETES 2004 EMSA, electrophoretic mobility shift assay GK, glucokinase GKRP, GK regulatory protein LGK, live type glucokinase PPAR, peroxisome proliferator-activated receptor PPRE, PPAR response element RXR, retinoid X...
Journal Articles
Journal: Diabetes
Diabetes 2001;50(3):622–629
Published: 01 March 2001
... of variance DTT, dithioerythritol G6Pase, glucose-6-phosphatase GK, glucokinase GKRP, GK regulatory protein HF diet, high-fat high–simple carbohydrate low-fiber diet NEFA, nonesterified fatty acid Diabetes is characterized by both fasting and excessive postprandial hyperglycemia ( 1 – 6 ). Affected...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(4):893–900
Published: 01 April 2002
... the counterregulatory hormones were at baseline, although the plasma glucose levels in this protocol were clamped above the threshold for triggering hypoglycemia counterregulation. GK activity is acutely regulated by its interaction with GK regulatory protein (GKRP). The regulatory protein located within...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(suppl_3):S394–S404
Published: 01 December 2002
... of the enzyme’s glucose sensor function in GK-containing cells, provided glucose transport is not rate limiting, a condition that seems to be fulfilled in the well-characterized glucose sensor cells and hepatocytes ( 1 , 4 ). In cells that contain GK regulatory protein (GKRP), the enzyme may be inhibited...
Journal Articles
Journal: Diabetes
Diabetes 1997;46(2):179–186
Published: 01 February 1997
...; DMEM, Dulbecco's modified Eagle medium; F-l-P, fructose-1-phosphate; F-6-P, fructose-6-phosphate; FBS, fetal bovine serum; GK, glucokinase; GKRP, glucokinase regulatory protein; IBMX, isobutylmethylxanthine; KH, Krebs-Henseleit buffer; L-PK, liver type pyruvate kinase; MODY, maturity onset diabetes...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(3):549–559
Published: 01 March 2004
... 2004 AUC, area under the curve [Ca2+]i, intracellular Ca2+ GABA, γ-aminobutyric acid GE, glucose excited GI, glucose inhibited GK, glucokinase GKRP, GK regulatory protein HK-I, hexokinase-I INS-R, insulin receptor KATP channel, ATP-sensitive...
Journal Articles
Journal: Diabetes
Diabetes 2017;66(5):1172–1184
Published: 28 February 2017
... to that of ribosomal protein L13a to control for nonspecific sample-to-sample variation. Glycogen synthase and phosphorylase a activities in the liver were measured using methods described previously ( 21 ). The immunoreactivities of both GK and GKRP within the nucleus and cytoplasm of each parenchymal cell were...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2009;58(1):78–86
Published: 01 January 2009
... in response to a rise in plasma glucose and insulin, as seen in the early (10 weeks of age) and middle (14 weeks of age) phases of diabetes development in ZDF rats, is associated with impaired regulation of GK by GK regulatory protein (GKRP) ( 15 – 17 ). In our current study, we investigated GK expression...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2013;62(2):392–400
Published: 17 January 2013
... regulatory protein [GKRP]) or Cell Signaling Technology (GS). Test protein bands were quantified using ImageJ software (National Institutes of Health; http://rsb.info.nih.gov/ij/ ). GK activity was assessed as described previously ( 28 ). GS activity ratio was calculated as activity measured at low...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2011;60(12):3175–3185
Published: 13 November 2011
... show compromised activity in pancreatic β-cells. Regulation of GK at the posttranslational level differs in the liver and pancreatic β-cells ( 14 ). In hepatocytes, GK is regulated by binding to the liver regulatory protein (GKRP) ( 15 ), which modulates GK activity by sequestration of inactive GK...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2009;58(1):18–20
Published: 01 January 2009
... in newly diagnosed type 2 diabetic patients ( 8 ) or decreased in obese subjects with diabetes ( 9 ). Hepatic glucokinase is regulated by an inhibitory protein (glucokinase regulatory protein [GKRP]) that binds glucokinase with high affinity at basal glucose concentrations (5 mmol/l) and sequesters...
Journal Articles
Journal: Diabetes
Diabetes 2003;52(6):1326–1332
Published: 01 June 2003
... of refeeding ( 30 ). (The half-lives of GK activity and GK mRNA are 30 h and 45 min respectively [ 30 , 31 ].) During fasting, GK is tightly bound to the inhibitory GK regulatory protein (GKRP) ( 32 ) in the nucleus ( 33 ) (except perivenously [ 13 ]). Farrelly et al. ( 34 ) and Grimsby et al. ( 35...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(9):2346–2352
Published: 01 September 2004
.... Glucokinase is also expressed in hepatocytes, where its catalytic activity is regulated by a 68-kDa inhibitory protein, glucokinase regulatory protein (GKRP) ( 5 ). Glucokinase is bound to GKRP within the nucleus in metabolically quiescent conditions and translocates to a free state in the cytoplasm...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(1):1–12
Published: 01 January 2006
..., University of Pennsylvania, Department of BiochemistryBiophysics, 501 Stemmler Hall, Philadelphia, PA 19104. E-mail: matsch@mail.med.upenn.edu 23 8 2005 20 7 2005 DIABETES 2006 GKA, glucokinase activator drug GKRP, glucokinase regulatory protein GSIR, glucose-stimulated insulin...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(6):1713–1722
Published: 01 June 2006
... the stability of G72R and G264S differed only slightly from that of wild type. G72R and M210K did not respond to an allosteric glucokinase activator (GKA) or the hepatic glucokinase regulatory protein (GKRP). Mutation analysis of the role of glycine at position 72 by substituting E, F, K, M, S, or Q showed...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(4):865–874
Published: 01 April 2006
...-P2, fructose 2,6-bisphosphate G6Pase, glucose-6-phosphatase GKRP, glucokinase regulatory protein Glc-6-P, glucose 6-phosphate HBM, hepatocyte basal medium PFK, 6-phosphofructokinase PKA, protein kinase A PKB, protein kinase B The control of glucose uptake depends on the relative rates...
Includes: Supplementary data