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hmw-high-molecular-weight

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Journal Articles
Journal: Diabetes
Diabetes 2006;55(7):1954–1960
Published: 01 July 2006
... resistance HMW, high molecular weight LMW, low molecular weight MMW, medium molecular weight Adiponectin, also known as Acrp30 ( 1 ), AdipoQ ( 2 ), gelatin-binding protein of 28 kDa (GBP28) ( 3 ), and apM1 ( 4 ), is an adipocyte-specific protein that enhances insulin sensitivity and promotes lipid...
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Biomarker correlation network comparing 1,303 case subjects with diabetes v...
Published: 14 January 2019
Biomarker correlation network comparing 1,303 case subjects with diabetes versus 1,627 noncase subjects without diabetes. ADPN, adiponectin; E-sel, E-selectin; HMW, high molecular weight. Biomarker correlation network comparing 1,303 case subjects with diabetes versus 1,627 noncase subjects withou... More
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Adenovirus-mediated adiponectin overexpression in mice. Purified adenovirus...
Published: 01 July 2008
FIG. 1. Adenovirus-mediated adiponectin overexpression in mice. Purified adenovirus vectors were injected into mice through the tail vein. Plasma adiponectin protein levels were measured by Western blot. A: Time course of total serum adiponectin protein in Ad-mACRP30 viral vector–treated mice. Top panel is a representative autoradiograph of four mice. B: Serum total adiponectin protein levels were increased (∼12-fold) 3 days after viral vector injection. C: Proportional increase of circulating multimeric forms of adiponectin in Ad-mACRP30 vector–treated mice. The multimeric forms of adiponectin were analyzed by nonreducing PAGE. HMW, high molecular weight; MMW, medium molecular weight; LMW, low molecular weight. FIG. 1. Adenovirus-mediated adiponectin overexpression in mice. Purified adenovirus vectors were injected into mice through the tail vein. Plasma adiponectin protein levels were measured by Western blot. A: Time course of total serum adiponectin protein in Ad-mACRP30 viral vector–treated mice. Top panel is a representative autoradiograph of four mice. B: Serum total adiponectin protein levels were increased (∼12-fold) 3 days after viral vector injection. C: Proportional increase of circulating multimeric forms of adiponectin in Ad-mACRP30 vector–treated mice. The multimeric forms of adiponectin were analyzed by nonreducing PAGE. HMW, high molecular weight; MMW, medium molecular weight; LMW, low molecular weight. More
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<span class="search-highlight">Weight</span> loss alters the expression and secretion of cytokines in adipocytes ...
Published: 17 May 2013
FIG. 3. Weight loss alters the expression and secretion of cytokines in adipocytes differentiated in vitro. Cells were differentiated for 12 days, and conditioned media and cells were collected after 24 h of incubation in freshly added media for analysis of cytokine expression and secretion. A: Effect of weight loss on cytokine gene expression. Quantitative RT-PCR analysis of mRNA for selected cytokines, normalized to GUSB expression (n = 15). B: Effect of weight loss on cytokine secretion. Fold change over the basal values for MCP1, IL-6, and adiponectin in conditioned media measured by ELISA, normalized to protein content (n = 22). C: Effect of weight loss on adiponectin isoform secretion. Quantification of adiponectin isoforms by native polyacrylamide gel electrophoresis and Western blot analysis (n = 21). Data are means ± SE; *P < 0.05, **P < 0.01, ***P < 0.001. HMW, high molecular weight; LMW, low molecular weight, MMW, medium molecular weight. FIG. 3. Weight loss alters the expression and secretion of cytokines in adipocytes differentiated in vitro. Cells were differentiated for 12 days, and conditioned media and cells were collected after 24 h of incubation in freshly added media for analysis of cytokine expression and secretion. A: Effect of weight loss on cytokine gene expression. Quantitative RT-PCR analysis of mRNA for selected cytokines, normalized to GUSB expression (n = 15). B: Effect of weight loss on cytokine secretion. Fold change over the basal values for MCP1, IL-6, and adiponectin in conditioned media measured by ELISA, normalized to protein content (n = 22). C: Effect of weight loss on adiponectin isoform secretion. Quantification of adiponectin isoforms by native polyacrylamide gel electrophoresis and Western blot analysis (n = 21). Data are means ± SE; *P < 0.05, **P < 0.01, ***P < 0.001. HMW, high molecular weight; LMW, low molecular weight, MMW, medium molecular weight. More
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Effect of fasting on blood glucose (<em>A</em>), insulin (<em>B</em>...
Published: 01 December 2006
FIG. 1. Effect of fasting on blood glucose (A), insulin (B and C), leptin, and adiponectin. Male C57Bl6/J mice (age 10 weeks) were deprived of food, and blood samples were drawn from the tail vein. Glucose was measured using a blood glucose meter (One Touch Ultra, Johnson & Johnson). Insulin and adiponectin were measured in serum by enzyme-linked immunosorbent assay and radioimmunoassay as described ( 29 ). The complex forms of adiponectin were resolved on 4–20% SDS-PAGE, transferred to nitrocellulose and blotted for adiponectin ( 30 , 31 ). D: C57Bl/6J mice were subjected to a hyperinsulinemic-euglycemic clamp (HI-EG) or hyperinsulinemic-hyperglycemic clamp (HI-HG) as described ( 32 ). Leptin and adiponectin were measured in serum. Data are means ± SE; n = 5–8. *P < 0.001 vs. PBS. HMW, high molecular weight; LMW, low molecular weight; MMW, middle molecular weight. FIG. 1. Effect of fasting on blood glucose (A), insulin (B and C), leptin, and adiponectin. Male C57Bl6/J mice (age 10 weeks) were deprived of food, and blood samples were drawn from the tail vein. Glucose was measured using a blood glucose meter (One Touch Ultra, Johnson & Johnson). Insulin and adiponectin were measured in serum by enzyme-linked immunosorbent assay and radioimmunoassay as described (29). The complex forms of adiponectin were resolved on 4–20% SDS-PAGE, transferred to nitrocellulose and blotted for adiponectin (30,31). D: C57Bl/6J mice were subjected to a hyperinsulinemic-euglycemic clamp (HI-EG) or hyperinsulinemic-hyperglycemic clamp (HI-HG) as described (32). Leptin and adiponectin were measured in serum. Data are means ± SE; n = 5–8. *P < 0.001 vs. PBS. HMW, high molecular weight; LMW, low molecular weight; MMW, middle molecular weight. More
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Effect of fish oil on plasma adiponectin concentrations. Mice were fed a co...
Published: 01 April 2006
FIG. 1. Effect of fish oil on plasma adiponectin concentrations. Mice were fed a control diet or isocaloric high-fat diets containing 27% safflower oil and 27, 14.5, or 8% fish oil. Total plasma adiponectin concentrations were determined in fed mice on day 0 before and days 2, 4, 8, and 15 during dietary regimen (A) and show the increment in plasma adiponectin concentrations from days 0 to 15 (B). C: Mice were fed a 27% fish oil diet that was replaced by a 27% safflower oil diet on day 8, and plasma adiponectin concentrations were determined on day 0 and day 8 of fish oil diet treatment and after discontinuation of fish oil until day 15. D: Nonreducing and denatured plasma adiponectin was determined by Western blotting. Mice were fed a control diet, 27% safflower oil, or 27% fish oil. HMW, high molecular weight (>300 kDa); MMW, middle molecular weight (hexamer, ∼167 kDa); LMW, low molecular weight (trimer, ∼67 kDa). Data are means ± SE with n = 10/group (A and B) and n = 6–8/group (C). P value indicates statistical difference from safflower oil diet. FIG. 1. Effect of fish oil on plasma adiponectin concentrations. Mice were fed a control diet or isocaloric high-fat diets containing 27% safflower oil and 27, 14.5, or 8% fish oil. Total plasma adiponectin concentrations were determined in fed mice on day 0 before and days 2, 4, 8, and 15 during dietary regimen (A) and show the increment in plasma adiponectin concentrations from days 0 to 15 (B). C: Mice were fed a 27% fish oil diet that was replaced by a 27% safflower oil diet on day 8, and plasma adiponectin concentrations were determined on day 0 and day 8 of fish oil diet treatment and after discontinuation of fish oil until day 15. D: Nonreducing and denatured plasma adiponectin was determined by Western blotting. Mice were fed a control diet, 27% safflower oil, or 27% fish oil. HMW, high molecular weight (>300 kDa); MMW, middle molecular weight (hexamer, ∼167 kDa); LMW, low molecular weight (trimer, ∼67 kDa). Data are means ± SE with n = 10/group (A and B) and n = 6–8/group (C). P value indicates statistical difference from safflower oil diet. More
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Biomarker correlation network comparing 1,303 diabetes case subjects vs. 1,...
Published: 08 November 2018
Figure 1 Biomarker correlation network comparing 1,303 diabetes case subjects vs. 1,627 noncase subjects. Significant edges were identified by the threshold of both |Δr| > 0.15 and z > 3.5 (equivalent to a two-sided P value <0.0005). The width of the edge is proportional to the corresponding difference in the biomarker correlation between case and noncase subjects. A solid edge indicates that the absolute value of the correlation is stronger in case than in noncase subjects, and a dashed edge indicates the opposite. The color of the edge indicates the direction of the correlation in case and noncase subjects: red, positive correlations in both case and noncase subjects; green, negative correlations in both case and noncase subjects; gray, opposite correlations between case and noncase subjects. ADPN, adiponectin; E-sel, E-selectin; HMW, high molecular weight. Figure 1. Biomarker correlation network comparing 1,303 diabetes case subjects vs. 1,627 noncase subjects. Significant edges were identified by the threshold of both |Δr| > 0.15 and z > 3.5 (equivalent to a two-sided P value <0.0005). The width of the edge is proportional to the corresponding difference in the biomarker correlation between case and noncase subjects. A solid edge indicates that the absolute value of the correlation is stronger in case than in noncase subjects, and a dashed edge indicates the opposite. The color of the edge indicates the direction of the correlation in case and noncase subjects: red, positive correlations in both case and noncase subjects; green, negative correlations in both case and noncase subjects; gray, opposite correlations between case and noncase subjects. ADPN, adiponectin; E-sel, E-selectin; HMW, high molecular weight. More
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Phenotype of <em>Reverbα<sup>−/−</sup></em> mice. <em>A</em>...
Published: 04 September 2014
Figure 1 Phenotype of Reverbα−/− mice. A and B: Body weight (A) and visceral WAT (B) mass of 12-week-old male Reverbα−/− mice and WT littermates maintained on an NC diet (n = 16–20/group). C: Histological sections of WAT from WT and Reverbα−/− mice fed NC and WT mice fed HFD to induce DIO. iNOS immunoreactivity demonstrates characteristic macrophage cuffs in WAT of WT DIO mice, which are virtually absent in Reverbα−/− mice despite their obese phenotype. D and E: Body weight (D) and intraperitoneal glucose tolerance test results (E) of WT and Reverbα−/− mice fed NC or HFD for 10 weeks (n = 5–6/group). F and G: Serum leptin (F) and adiponectin (G) concentrations of 12-week-old Reverbα−/− mice were significantly higher than in WT controls (n = 18–21/group) and positively correlated with adipose tissue mass (P < 0.05, Pearson correlation). H: ELISA analysis of serum adiponectin in WT and Reverbα−/− mice fed NC vs. HFD. I: Immunoblot analysis of monomeric (top) and multimeric (bottom) adiponectin in sera of WT and Reverbα−/− mice (black arrowhead, high molecular weight; white arrowhead, hexamer; gray arrowhead, trimer). J: ELISA analysis of high–molecular weight adiponectin in sera of WT and Reverbα−/− mice expressed as a ratio of total adiponectin. Data are mean ± SEM; statistical significance was determined using Student t test or one-way ANOVA with Bonferroni post hoc test (C). *P < 0.05, **P < 0.01, ***P < 0.001. Adn, adiponectin; BW, body weight; HMW, high molecular weight. Figure 1. Phenotype of Reverbα−/− mice. A and B: Body weight (A) and visceral WAT (B) mass of 12-week-old male Reverbα−/− mice and WT littermates maintained on an NC diet (n = 16–20/group). C: Histological sections of WAT from WT and Reverbα−/− mice fed NC and WT mice fed HFD to induce DIO. iNOS immunoreactivity demonstrates characteristic macrophage cuffs in WAT of WT DIO mice, which are virtually absent in Reverbα−/− mice despite their obese phenotype. D and E: Body weight (D) and intraperitoneal glucose tolerance test results (E) of WT and Reverbα−/− mice fed NC or HFD for 10 weeks (n = 5–6/group). F and G: Serum leptin (F) and adiponectin (G) concentrations of 12-week-old Reverbα−/− mice were significantly higher than in WT controls (n = 18–21/group) and positively correlated with adipose tissue mass (P < 0.05, Pearson correlation). H: ELISA analysis of serum adiponectin in WT and Reverbα−/− mice fed NC vs. HFD. I: Immunoblot analysis of monomeric (top) and multimeric (bottom) adiponectin in sera of WT and Reverbα−/− mice (black arrowhead, high molecular weight; white arrowhead, hexamer; gray arrowhead, trimer). J: ELISA analysis of high–molecular weight adiponectin in sera of WT and Reverbα−/− mice expressed as a ratio of total adiponectin. Data are mean ± SEM; statistical significance was determined using Student t test or one-way ANOVA with Bonferroni post hoc test (C). *P < 0.05, **P < 0.01, ***P < 0.001. Adn, adiponectin; BW, body weight; HMW, high molecular weight. More
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Reduced n-6/n-3 PUFA ratio exposure leads to smaller and more numerous subc...
Published: 14 November 2017
Figure 3 Reduced n-6/n-3 PUFA ratio exposure leads to smaller and more numerous subcutaneous adipocytes and higher systemic adiponectin. A: Quantification of enzymatically digested sWAT from PND14 offspring. Exposure to a low n-6/n-3 PUFA ratio results in an adipocyte cellularity profile with a greater percentage of small-sized and fewer large-sized adipocytes (n = 29 WT pups from five WT dams; n = 18 WT pups from five fat-1 dams). B: Total adipocyte number per gram of sWAT and overall sWAT weight were both greater in low n-6/n-3 PUFA ratio offspring (P = 0.01 and 0.005, respectively; same number of pups and dams as in A). C: Systemic adiponectin, specifically the low-molecular-weight (LMW) isoforms, was significantly increased in response to a low n-6/n-3 PUFA ratio in PND14 pups (P < 0.001), whereas systemic glucose, insulin, and leptin levels were equivalent (n = 8 WT pups from four WT dams; n = 8 WT pups from five fat-1 dams). *P < 0.05. HMW, high molecular weight. Figure 3. Reduced n-6/n-3 PUFA ratio exposure leads to smaller and more numerous subcutaneous adipocytes and higher systemic adiponectin. A: Quantification of enzymatically digested sWAT from PND14 offspring. Exposure to a low n-6/n-3 PUFA ratio results in an adipocyte cellularity profile with a greater percentage of small-sized and fewer large-sized adipocytes (n = 29 WT pups from five WT dams; n = 18 WT pups from five fat-1 dams). B: Total adipocyte number per gram of sWAT and overall sWAT weight were both greater in low n-6/n-3 PUFA ratio offspring (P = 0.01 and 0.005, respectively; same number of pups and dams as in A). C: Systemic adiponectin, specifically the low-molecular-weight (LMW) isoforms, was significantly increased in response to a low n-6/n-3 PUFA ratio in PND14 pups (P < 0.001), whereas systemic glucose, insulin, and leptin levels were equivalent (n = 8 WT pups from four WT dams; n = 8 WT pups from five fat-1 dams). *P < 0.05. HMW, high molecular weight. More
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Biomarker correlation network comparing diabetes case subjects diagnosed du...
Published: 08 November 2018
Figure 2 Biomarker correlation network comparing diabetes case subjects diagnosed during different time periods after blood collection vs. noncase subjects. A: Case subjects (n = 501) diagnosed >10 years after blood collection vs. 1,627 noncase subjects. B: Case subjects (n = 491) diagnosed 5–10 years after blood collection vs. 1,627 noncase subjects. C: Case subjects (n = 311) diagnosed <5 years after blood collection vs. 1,627 noncase subjects. Significant edges were identified by the threshold of both |Δr| > 0.15 and z > 3.5 (equivalent to a two-sided P value <0.0005). The width of the edge is proportional to the corresponding difference in the biomarker correlation between case and noncase subjects. A solid edge indicates that the absolute value of the correlation is stronger in case than in noncase subjects, and a dashed edge indicates the opposite. The color of the edge indicates the direction of the correlation in case and noncase subjects: red, positive correlations in both case and noncase subjects; green, negative correlations in both case and noncase subjects; gray, opposite correlations between case and noncase subjects. ADPN, adiponectin; E-sel, E-selectin; HCO3, bicarbonate; HGB, hemoglobin; HMW, high molecular weight; sTNFR2, soluble tumor necrosis factor receptor type 2; vitd, vitamin D. Figure 2. Biomarker correlation network comparing diabetes case subjects diagnosed during different time periods after blood collection vs. noncase subjects. A: Case subjects (n = 501) diagnosed >10 years after blood collection vs. 1,627 noncase subjects. B: Case subjects (n = 491) diagnosed 5–10 years after blood collection vs. 1,627 noncase subjects. C: Case subjects (n = 311) diagnosed <5 years after blood collection vs. 1,627 noncase subjects. Significant edges were identified by the threshold of both |Δr| > 0.15 and z > 3.5 (equivalent to a two-sided P value <0.0005). The width of the edge is proportional to the corresponding difference in the biomarker correlation between case and noncase subjects. A solid edge indicates that the absolute value of the correlation is stronger in case than in noncase subjects, and a dashed edge indicates the opposite. The color of the edge indicates the direction of the correlation in case and noncase subjects: red, positive correlations in both case and noncase subjects; green, negative correlations in both case and noncase subjects; gray, opposite correlations between case and noncase subjects. ADPN, adiponectin; E-sel, E-selectin; HCO3, bicarbonate; HGB, hemoglobin; HMW, high molecular weight; sTNFR2, soluble tumor necrosis factor receptor type 2; vitd, vitamin D. More
Journal Articles
Journal: Diabetes
Diabetes 2007;56(8):2174–2177
Published: 01 August 2007
... not differ during the high-dose insulin infusions ( Fig. 1B ). HMW, high molecular weight LMW, low molecular weight Adiponectin is a secretory protein uniquely produced by adipocytes ( 1 ) and accepted as a marker for systemic insulin sensitivity, particularly as an indicator of hepatic...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(9):2712–2719
Published: 01 September 2005
... to 6.1 ± 0.6 μg/ml (P = 0.076). High (HMW) and medium molecular weight (MMW) adiponectin oligomers significantly increased during weight reduction (HMW: 0.37 ± 0.07 to 0.4 ± 0.08 μg/ml, P = 0.042; MMW: 2.3 ± 0.2 to 2.9 ± 0.3 μg/ml, P = 0.007), while low molecular weight (LMW...
Journal Articles
Journal: Diabetes
Diabetes 2006;55(1):249–259
Published: 01 January 2006
...Cristina Lara-Castro; Nanlan Luo; Penny Wallace; Richard L. Klein; W. Timothy Garvey Adiponectin circulates in human plasma mainly as a 180-kDa low molecular weight (LMW) hexamer and a high molecular weight (HMW) multimer of ∼360 kDa. We comprehensively examined the relationships between...
Journal Articles
Journal: Diabetes
Diabetes 2019;68(2):237–238
Published: 14 January 2019
...Biomarker correlation network comparing 1,303 case subjects with diabetes versus 1,627 noncase subjects without diabetes. ADPN, adiponectin; E-sel, E-selectin; HMW, high molecular weight. Biomarker correlation network comparing 1,303 case subjects with diabetes versus 1,627 noncase subjects...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(6):1712–1717
Published: 01 June 2007
...Robert K. Semple; Nils H. Halberg; Keith Burling; Maria A. Soos; Todd Schraw; Jian'an Luan; Elaine K. Cochran; David B. Dunger; Nicholas J. Wareham; Philipp E. Scherer; Phillip Gorden; Stephen O'Rahilly Total plasma adiponectin and highmolecular weight (HMW) polymeric adiponectin are strongly...
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Effect of iNOS gene disruption and rosiglitazone treatment on plasma adipon...
Published: 01 August 2008
FIG. 4. Effect of iNOS gene disruption and rosiglitazone treatment on plasma adiponectin and adipose tissue remodeling in high-fat–fed obese mice. A: Total adiponectin concentrations (□) and high–molecular weight adiponectin complexes (HMW, ▪) were measured in plasma by radioimmunoassay and size fractionation followed by quantitative Western blotting, respectively. The dotted lines corresponds to total and high–molecular weight adiponectin levels in untreated chow-fed iNOS+/+ mice. B: Histological analysis of WAT of high-fat–fed animals. Mean weight and cell number per fat pad are also shown. Bars represent the means ± SE of 5–16 mice per group. *P < 0.05 vs. untreated mice, #P < 0.05 vs. iNOS+/+ mice. FIG. 4. Effect of iNOS gene disruption and rosiglitazone treatment on plasma adiponectin and adipose tissue remodeling in high-fat–fed obese mice. A: Total adiponectin concentrations (□) and high–molecular weight adiponectin complexes (HMW, ▪) were measured in plasma by radioimmunoassay and size fractionation followed by quantitative Western blotting, respectively. The dotted lines corresponds to total and high–molecular weight adiponectin levels in untreated chow-fed iNOS+/+ mice. B: Histological analysis of WAT of high-fat–fed animals. Mean weight and cell number per fat pad are also shown. Bars represent the means ± SE of 5–16 mice per group. *P < 0.05 vs. untreated mice, #P < 0.05 vs. iNOS+/+ mice. More
Journal Articles
Journal: Diabetes
Diabetes 2004;53(6):1621–1629
Published: 01 June 2004
... known actions of TZDs, increased circulating levels of the high molecular weight (HMW) multimer of adiponectin may be an important insulin-sensitizing mechanism. We examined the effects of only 21 days of 45 mg of pioglitazone (P+) versus placebo (P−) in nine subjects with type 2 diabetes...
Meeting Abstracts
Journal: Diabetes
Diabetes 2019;68(Supplement_1):1970-P
Published: 01 June 2019
...SALIHA MUSOVIC; ALI M. KOMAI; ELIN BANKE NORDBECK; UMUL-KHAIR A. NOOR; INGRID WERNSTEDT ASTERHOLM; CHARLOTTA S. OLOFSSON The white adipocyte hormone adiponectin is released in different molecular forms and a reduction of high-molecular weight (HMW) adiponectin has specifically been associated...
Journal Articles
Journal: Diabetes
Diabetes 2019;68(2):281–290
Published: 08 November 2018
... of the correlation in case and noncase subjects: red, positive correlations in both case and noncase subjects; green, negative correlations in both case and noncase subjects; gray, opposite correlations between case and noncase subjects. ADPN, adiponectin; E-sel, E-selectin; HMW, high molecular weight. Figure 1...
Includes: Supplementary data
Meeting Abstracts
Journal: Diabetes
Diabetes 2020;69(Supplement_1):1573-P
Published: 01 June 2020
..., we investigated the association between serum high-molecular-weight (HMV) adiponectin and proinsulin levels in a general Japanese population. Methods: In this cross-sectional study, we analyzed data for residents aged between 35 and 79 years in a rural community in Hokkaido, Japan. A total of 488...