1-20 of 536 Search Results for

hplc-high-performance-liquid-chromatography

Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Journal Articles
Journal: Diabetes
Diabetes 1995;44(4):437–440
Published: 01 April 1995
...-insulin-dependent dia- bined with high-performance liquid chromatography betes mellitus (NIDDM) and impaired glucose tolerance (HPLC), which can discriminate between 32-33 split, (4,5). Knowledge of proinsulin processing in these condi- des-32 split, and des-31,32 split proinsulin. Subjects were tions may...
Journal Articles
Journal: Diabetes
Diabetes 1997;46(1):44–50
Published: 01 January 1997
... as an internal standard and added to the serum samples before sample processing. After isolation by immunoaffinity chromatography and solid phase extraction, followed by a purification step on reversed-phase microbore high-performance liquid chromatography (HPLC), the insulincontaining fraction was analyzed...
Journal Articles
Journal: Diabetes
Diabetes 1994;43(2):297–304
Published: 01 February 1994
.... Cellular and plasma radioactivity was characterized by high-performance liquid chromatography (HPLC). The results of the study show the following: 1) Products with superimposable HPLC elution profiles are found within cells and in medium. Two new labeled products are observed in the latter...
Journal Articles
Journal: Diabetes
Diabetes 1997;46(11):1725–1732
Published: 01 November 1997
... the physiology jects; high-performance liquid chromatography (HPLC) and pathophysiology of pancreatic P-cell func- was also performed in a subset of these subjects to mea- tion. Although various animal models of sure the relative contribution of intact proinsulin and its conversion intermediates to total...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(9):2833–2839
Published: 01 September 2002
..., Nε-(carboxymethyl)lysine; DAN, 2,3-diaminonaphthalene; ELISA, enzyme-linked immunosorbent assay; ESI-MS, electrospray ionization-mass spectrometry; HPLC, high-performance liquid chromatography; HRP, horseradish peroxidase; HSA, human serum albumin; iNOS, inducible nitric oxide synthase; KLH, keyhole...
Meeting Abstracts
Journal: Diabetes
Diabetes 2019;68(Supplement_1):210-LB
Published: 01 June 2019
... with the efforts of many national and international organizations; however, in some regions, high performing assays might not be readily available. Here we report a comparison study of two immunoassay methods with a high-performance liquid chromatography (HPLC) assay, all of them available in Ecuador. We studied...
Journal Articles
Journal: Diabetes
Diabetes 1989;38(11):1478–1483
Published: 01 November 1989
... of a monosialoganglioside (GM). To characterize islet glycolipids and ascertain whether islets express unique gangliosides, we determined the pattern of ganglioside expression in whole human pancreas and isolated human islets using high-performance liquid chromatography (HPLC) and high-performance thin-layer...
Journal Articles
Journal: Diabetes
Diabetes 1983;32(5):474–477
Published: 01 May 1983
...F B Stentz; H L Harris; A E Kitabchi We studied the metabolism of A14-125I-Insulin in intact human fibroblasts using high performance liquid chromatography (HPLC) to detect and separate its early degradation products. The high resolving power of HPLC enabled us to separate...
Journal Articles
Journal: Diabetes
Diabetes 1991;40(4):436–443
Published: 01 April 1991
... incubated in various buffers, and hormone degradation was monitored by Sephadex G-50 chromatography and high-performance liquid chromatography (HPLC). Endosomes incubated in simple imidazole or HEPES (pH 7.4) buffers rapidly degraded insulin to intermediate- and then to low-molecular-weight products...
Journal Articles
Journal: Diabetes
Diabetes 1990;39(5):563–569
Published: 01 May 1990
...Hitoshi Sato; Akira Tsuji; Ken-Ichi Hirai; Young Sook Kang To describe quantitatively the in vivo distribution and elimination of insulin, high-performance liquid chromatography (HPLC) separation was applied to the pharmacokinetic study of human insulin labeled with 125I at tyrosine A14...
Journal Articles
Journal: Diabetes
Diabetes 1982;31(12):1128–1131
Published: 01 December 1982
... labeled insulins were further purified by either DEAE cellulose or high performance liquid chromatography (HPLC) to separate A14-125I- from A19-125I-insulin. Both methods of chromatography were effective, but HPLC offered the advantage of better...
Journal Articles
Journal: Diabetes
Diabetes 1991;40(8):959–965
Published: 01 August 1991
... BPs by isocratic high-performance liquid chromatography (HPLC) at pH 3.9, and free IGF-I was estimated operationally as immunoreactivity with molecular weight equal to native IGF-I after HPLC at pH 7. Animals given 36 mg/kg STZ exhibited a glucose level of 9.74 mM and impaired weight gain, with little...
Journal Articles
Journal: Diabetes
Diabetes 1990;39(6):728–733
Published: 01 June 1990
... no lymphoblast transformation when crystalline insulin was used or when commercial preparations were purified to a single peak by high-performance liquid chromatography (HPLC). Pure crystalline insulin dimers of beef, pork, and human species were tested; control subjects responded with mean Sis of 1.9,1.9...
Meeting Abstracts
Journal: Diabetes
Diabetes 2018;67(Supplement_1):2168-P
Published: 01 July 2018
... functional loss are still unknown. The aim of the study is to highlight changes in the proteome of pancreatic human islets that can anticipate and eventually predict diabetes’ onset. High performance liquid chromatography mass spectrometry (HPLC-MS) analysis was applied to islets isolated by laser capture...
Journal Articles
Journal: Diabetes
Diabetes 1985;34(5):510–519
Published: 01 May 1985
... (91.0 ± 1.4%) and 9000 (9.0 ± 1.4%) molecular weight (mol wt) material. By 10% polyacrylamide disc gel electrophoresis (PAGE) and reverse-phase, high-performance liquid chromatography (HPLC), the 6000 mol wt material was indistinguishable from human insulin standards and insulin fragments were not found...
Journal Articles
Journal: Diabetes
Diabetes 1991;40(9):1218–1222
Published: 01 September 1991
... the radio- filtration on a Bio-Gel P-6 column and finally chemical center (Amersham, Aylesbury, UK). Crude colla- purified by reverse-phase high-performance liquid genase (type II), somatostatin, and bovine albumin (fraction chromatography (HPLC) on a C-18 column. The V) were from Sigma (St. Louis, MO...
Journal Articles
Journal: Diabetes
Diabetes 1986;35(6):654–661
Published: 01 June 1986
.... However, high-performance liquid chromatography (HPLC) and trichloroacetic acid (TCA) analysis of the released material showedthat it consisted mostly of degraded peptide. These studies show that brain capillaries have separate receptors for insulin, IGF I, and IGFII. The endocytosis of these peptides...
Journal Articles
Journal: Diabetes
Diabetes 1986;35(1):52–57
Published: 01 January 1986
... or an anti-BAM-22P (bovine adrenal medulla docosapeptide) antiserum was used. The peptides were characterized by a combination of gel-filtration chromatography, high-performance liquid chromatography (HPLC), and specific radioimmunoassay. Free met-enkephalin content in extracts of rat islets was 90-fold...
Journal Articles
Journal: Diabetes
Diabetes 1983;32(8):705–711
Published: 01 August 1983
... and Company, Indianapolis, Indiana of reverse phase high performance liquid chromatography 46285. (HPLC) as a more rapid and generally applicable method Received for publication 15 September 1982 and in revised form 3 February 1983. DIABETES, VOL. 32, AUGUST 1983 705 RECEPTOR BINDING OF MONOIODOINSULIN...
Journal Articles
Journal: Diabetes
Diabetes 1985;34(3):212–216
Published: 01 March 1985
... a direct role in fetal erythropoiesis in diabetic preg- embryonic globin chains was studied by separation of nancy. However, it has also been suggested that the effects the globin chains by high-performance liquid chroma- of high levels of insulin on erythropoiesis may be secondary tography (HPLC...