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igtt-intraperitoneal-glucose-tolerance-test

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<span class="search-highlight">Intraperitoneal</span> <span class="search-highlight">glucose</span> <span class="search-highlight">tolerance</span> <span class="search-highlight">tests</span> (<span class="search-highlight">IGTTs</span>) after exendin-4 administrat...
Published: 16 April 2013
FIG. 7. Intraperitoneal glucose tolerance tests (IGTTs) after exendin-4 administration. Overnight-fasted β-caPKA and wild-type (WT) littermate control mice were administered exendin-4 15 min before receiving an 2 g/kg i.p. glucose bolus. Plasma insulin was measured over the subsequent 20 min (0–20 min) and blood glucose was measured over the −15- to 120-min interval. Area under the curve (AUC) was calculated for each curve relative to a y-axis value of 0. Diamonds, saline treated; squares, exendin-4 treated; open symbols and bars, controls; closed symbols and bars, β-caPKA. Two-way ANOVA indicated significant difference between IGTT and oral glucose tolerance test (P < 0.05) in (AD). Bonferroni posttest analysis: *P < 0.05; **P < 0.01; ***P < 0.001. FIG. 7. Intraperitoneal glucose tolerance tests (IGTTs) after exendin-4 administration. Overnight-fasted β-caPKA and wild-type (WT) littermate control mice were administered exendin-4 15 min before receiving an 2 g/kg i.p. glucose bolus. Plasma insulin was measured over the subsequent 20 min (0–20 min) and blood glucose was measured over the −15- to 120-min interval. Area under the curve (AUC) was calculated for each curve relative to a y-axis value of 0. Diamonds, saline treated; squares, exendin-4 treated; open symbols and bars, controls; closed symbols and bars, β-caPKA. Two-way ANOVA indicated significant difference between IGTT and oral glucose tolerance test (P < 0.05) in (A–D). Bonferroni posttest analysis: *P < 0.05; **P < 0.01; ***P < 0.001. More
Images
Axitinib increases thermogenesis in vivo. <em>A</em>: Live visualiz...
Published: 08 November 2016
Figure 7 Axitinib increases thermogenesis in vivo. A: Live visualization and quantification of luciferase activity in Ucp1-2A-luciferase mice treated with vehicle or axitinib for 8 weeks. Representative mice are shown. B–E: Quantification of luminescence in A for the dorsal, ventral upper body, ventral lower body, and side view images. F: Axitinib limited body weight gain in Ucp1-2A-luciferase mice fed an HFD. G and H: Axitinib improved glucose clearance in Ucp1-2A-luciferase mice (n = 9). I: Axitinib reduced the weight of ingWAT, eWAT, and liver in Ucp1-2A-luciferase mice. J: Relative luciferase activities in response to axitinib showed enhanced Ucp1 expression. K: UCP1 immunostaining in iBAT and ingWAT from Ucp1-2A-luciferase mice fed an HFD treated with vehicle or axitinib. L: H-E staining of fatty liver from mice treated with vehicle or axitinib. *P < 0.05, **P < 0.01, compared with control group (n = 9). IGTT, intraperitoneal glucose tolerance test; ITT, insulin tolerance test; NC, nontreated control; ND, not determined; RLU, relative light unit; W, week. Figure 7. Axitinib increases thermogenesis in vivo. A: Live visualization and quantification of luciferase activity in Ucp1-2A-luciferase mice treated with vehicle or axitinib for 8 weeks. Representative mice are shown. B–E: Quantification of luminescence in A for the dorsal, ventral upper body, ventral lower body, and side view images. F: Axitinib limited body weight gain in Ucp1-2A-luciferase mice fed an HFD. G and H: Axitinib improved glucose clearance in Ucp1-2A-luciferase mice (n = 9). I: Axitinib reduced the weight of ingWAT, eWAT, and liver in Ucp1-2A-luciferase mice. J: Relative luciferase activities in response to axitinib showed enhanced Ucp1 expression. K: UCP1 immunostaining in iBAT and ingWAT from Ucp1-2A-luciferase mice fed an HFD treated with vehicle or axitinib. L: H-E staining of fatty liver from mice treated with vehicle or axitinib. *P < 0.05, **P < 0.01, compared with control group (n = 9). IGTT, intraperitoneal glucose tolerance test; ITT, insulin tolerance test; NC, nontreated control; ND, not determined; RLU, relative light unit; W, week. More
Meeting Abstracts
Journal: Diabetes
Diabetes 2020;69(Supplement_1):2085-P
Published: 01 June 2020
... of the refeeding period. We administered three weekly cycles of FMD (1st day 50%-, 2nd-3rd day 10% of daily calorie intake) followed by 4 days of refeeding ad libitum after every cycle to 20 mice at 12 weeks of age. In the second FMD cycle an intraperitoneal glucose tolerance test...
Images
Physiological analysis of Hep-M3-KO mice (■) and control littermates (□) ma...
Published: 14 September 2009
FIG. 4. Physiological analysis of Hep-M3-KO mice (■) and control littermates (□) maintained on a high-fat diet. A: Growth curves. B: Fed blood glucose levels of male mice maintained on a high-fat diet (n = 7–9 per group). C: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). D: Blood glucose levels following oral administration of 2 mg/g of glucose (OGTT; 20-week-old males, n = 6 per group). E: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (18-week-old males, n = 6 per group). F: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (18-week-old males, n = 6 per group). G: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (22-week-old males, n = 6 per group). *P < 0.05 vs. control. FIG. 4. Physiological analysis of Hep-M3-KO mice (■) and control littermates (□) maintained on a high-fat diet. A: Growth curves. B: Fed blood glucose levels of male mice maintained on a high-fat diet (n = 7–9 per group). C: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). D: Blood glucose levels following oral administration of 2 mg/g of glucose (OGTT; 20-week-old males, n = 6 per group). E: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (18-week-old males, n = 6 per group). F: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (18-week-old males, n = 6 per group). G: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (22-week-old males, n = 6 per group). *P < 0.05 vs. control. More
Images
Physiological analysis of Hep-M3-Tg mice (■) and wild-type (WT) littermates...
Published: 14 September 2009
FIG. 8. Physiological analysis of Hep-M3-Tg mice (■) and wild-type (WT) littermates (□) maintained on a high-fat diet. A: Growth curves. B: Fed blood glucose levels of male mice maintained on a high-fat diet (n = 7–9 per group). C: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). D: Blood glucose levels following oral administration of 2 mg/g of glucose (OGTT); 18-week-old males, n = 6 per group). E: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (20-week-old males, n = 7 per group). F: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (22-week-old males, n = 7 per group). G: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (14-week-old males, n = 7 per group). FIG. 8. Physiological analysis of Hep-M3-Tg mice (■) and wild-type (WT) littermates (□) maintained on a high-fat diet. A: Growth curves. B: Fed blood glucose levels of male mice maintained on a high-fat diet (n = 7–9 per group). C: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). D: Blood glucose levels following oral administration of 2 mg/g of glucose (OGTT); 18-week-old males, n = 6 per group). E: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (20-week-old males, n = 7 per group). F: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (22-week-old males, n = 7 per group). G: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (14-week-old males, n = 7 per group). More
Images
Physiological analysis of Hep-M3-Tg mice (■) and wild-type (WT) littermates...
Published: 14 September 2009
FIG. 6. Physiological analysis of Hep-M3-Tg mice (■) and wild-type (WT) littermates (□) maintained on regular diet. A: Growth curves of male mice. B: Fed and fasting blood glucose. C: Serum insulin levels of Hep-M3-Tg and WT control mice maintained on regular diet. Blood samples were taken from freely fed mice or from mice that had been fasted for 15 h (3- and 9-month-old males; n = 7–9 per group). Hep-M3-Tg mice (■) and WT control littermates (□). D: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). E: Blood glucose following oral administration of 2 mg/g of glucose (OGTT; 20-week-old males, n = 6 per group). F: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (16-week-old males, n = 7 per group). G: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (20-week-old males, n = 7 per group). H: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (18-week-old males, n = 7 per group). Hep-M3-Tg mice (■) and WT littermates (□). FIG. 6. Physiological analysis of Hep-M3-Tg mice (■) and wild-type (WT) littermates (□) maintained on regular diet. A: Growth curves of male mice. B: Fed and fasting blood glucose. C: Serum insulin levels of Hep-M3-Tg and WT control mice maintained on regular diet. Blood samples were taken from freely fed mice or from mice that had been fasted for 15 h (3- and 9-month-old males; n = 7–9 per group). Hep-M3-Tg mice (■) and WT control littermates (□). D: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). E: Blood glucose following oral administration of 2 mg/g of glucose (OGTT; 20-week-old males, n = 6 per group). F: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (16-week-old males, n = 7 per group). G: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (20-week-old males, n = 7 per group). H: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (18-week-old males, n = 7 per group). Hep-M3-Tg mice (■) and WT littermates (□). More
Journal Articles
Journal: Diabetes
Diabetes 2008;57(6):1517–1525
Published: 01 June 2008
... using a global thresholding technique. AUC, area under the curve BMD, bone mineral density DβH, dopamine–β-hydroxylase H-E, hematoxylin-eosin IGTT, intraperitoneal glucose tolerance test IRES, internal ribosomal entry site NE, norepinephrine NPY, neuropeptide Y RIA, radioimmunoassay SNS...
Images
Physiological analysis of Hep-M3-KO mice (■) and control littermates (□) ma...
Published: 14 September 2009
FIG. 2. Physiological analysis of Hep-M3-KO mice (■) and control littermates (□) maintained on regular diet. A: Growth curves of male mice. B: Body composition (4-month-old males; control, n = 9; Hep-M3-KO, n = 6). C: Fed and fasting blood glucose. D: Serum insulin levels of Hep-M3-KO mice and control littermates. Blood samples were taken from freely fed mice or from mice that had been fasted for 12 h (3- and 8-month-old males; n = 7–9 per group). E: Insulin clamp studies. Rates of glucose uptake (RD, glucose disposal), GIR, and endogenous glucose production (GP) are indicated (4-month-old males; n = 3 per group). F: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). G: Blood glucose levels following oral administration of 2 mg/g of glucose (OGTT; 20-week-old males, n = 6 per group). H: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (18-week-old males, n = 6 per group). I: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (18-week-old males, n = 6 per group). J: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (22-week-old males, n = 6 per group). *P < 0.05 vs. control. FIG. 2. Physiological analysis of Hep-M3-KO mice (■) and control littermates (□) maintained on regular diet. A: Growth curves of male mice. B: Body composition (4-month-old males; control, n = 9; Hep-M3-KO, n = 6). C: Fed and fasting blood glucose. D: Serum insulin levels of Hep-M3-KO mice and control littermates. Blood samples were taken from freely fed mice or from mice that had been fasted for 12 h (3- and 8-month-old males; n = 7–9 per group). E: Insulin clamp studies. Rates of glucose uptake (RD, glucose disposal), GIR, and endogenous glucose production (GP) are indicated (4-month-old males; n = 3 per group). F: Blood glucose levels following intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). G: Blood glucose levels following oral administration of 2 mg/g of glucose (OGTT; 20-week-old males, n = 6 per group). H: Insulin tolerance test (ITT). Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 0.75 units/kg of insulin (18-week-old males, n = 6 per group). I: Glucagon tolerance test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 16 μg/kg of glucagon (18-week-old males, n = 6 per group). J: Pyruvate challenge test. Blood glucose levels were measured at the indicated time points after intraperitoneal injection of 2 mg/g of sodium pyruvate (22-week-old males, n = 6 per group). *P < 0.05 vs. control. More
Images
Single in vivo administration of the ERβ agonist (WAY200070) improved gluco...
Published: 17 May 2013
FIG. 2. Single in vivo administration of the ERβ agonist (WAY200070) improved glucose tolerance. A: Fasted C57BL/6 male mice were injected intraperitoneally with a single dose of WAY200070 3 mg/kg (W3), 10 mg/kg (W10), or 30 mg/kg (W30). In parallel, they were administered a glucose challenge (2 g/kg). Through an IGTT we observed an improvement in glucose response in animals treated with the ERβ agonist at W10. Thus, the AUC (inset) was significantly reduced in this group (n = 5). B: In addition, we measured plasma insulin levels 30 min after the administration of the glucose challenge and the ERβ agonist and detected that glucose-stimulated insulin release was significantly higher in W10 mice than in controls (8–10 mice/group). C: In the fasted state, a single injection of WAY200070 did not have an effect on glucose sensitivity (5 mice/group). We confirmed that this finding was an ERβ-mediated effect by using WT and BERKO mice. D: Fasted WT mice were injected intraperitoneally with a single dose of W10 or vehicle in parallel with a glucose challenge of 2 g/kg. A better response to the glucose load was observed in the animals that received the agonist, with a decreased AUC (inset) (5–7 mice/group). E: No changes in glucose tolerance or the AUC were observed in BERKO mice in response to the agonist W10-treated mice compared with the vehicle-treated mice. F: Glucose-stimulated insulin release was clearly enhanced in W10 WT mice but not in W10 BERKO mice (5–7 mice/group). Data are mean ± SE. *P < 0.05 versus vehicle (Student t test). In A and C, statistical analysis between groups was evaluated by one-way ANOVA, with P < 0.05 considered significant. BERKO, ERβ knockout. FIG. 2. Single in vivo administration of the ERβ agonist (WAY200070) improved glucose tolerance. A: Fasted C57BL/6 male mice were injected intraperitoneally with a single dose of WAY200070 3 mg/kg (W3), 10 mg/kg (W10), or 30 mg/kg (W30). In parallel, they were administered a glucose challenge (2 g/kg). Through an IGTT we observed an improvement in glucose response in animals treated with the ERβ agonist at W10. Thus, the AUC (inset) was significantly reduced in this group (n = 5). B: In addition, we measured plasma insulin levels 30 min after the administration of the glucose challenge and the ERβ agonist and detected that glucose-stimulated insulin release was significantly higher in W10 mice than in controls (8–10 mice/group). C: In the fasted state, a single injection of WAY200070 did not have an effect on glucose sensitivity (5 mice/group). We confirmed that this finding was an ERβ-mediated effect by using WT and BERKO mice. D: Fasted WT mice were injected intraperitoneally with a single dose of W10 or vehicle in parallel with a glucose challenge of 2 g/kg. A better response to the glucose load was observed in the animals that received the agonist, with a decreased AUC (inset) (5–7 mice/group). E: No changes in glucose tolerance or the AUC were observed in BERKO mice in response to the agonist W10-treated mice compared with the vehicle-treated mice. F: Glucose-stimulated insulin release was clearly enhanced in W10 WT mice but not in W10 BERKO mice (5–7 mice/group). Data are mean ± SE. *P < 0.05 versus vehicle (Student t test). In A and C, statistical analysis between groups was evaluated by one-way ANOVA, with P < 0.05 considered significant. BERKO, ERβ knockout. More
Journal Articles
Journal: Diabetes
Diabetes 2013;62(6):2015–2025
Published: 17 May 2013
... in vivo administration of the ERβ agonist (WAY200070) improved glucose tolerance. A: Fasted C57BL/6 male mice were injected intraperitoneally with a single dose of WAY200070 3 mg/kg (W3), 10 mg/kg (W10), or 30 mg/kg (W30). In parallel, they were administered a glucose challenge (2 g/kg). Through an IGTT...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2009;58(12):2776–2787
Published: 14 September 2009
... intraperitoneal administration of 2 mg/g of glucose (IGTT; 16-week-old males, n = 6 per group). D: Blood glucose levels following oral administration of 2 mg/g of glucose (OGTT; 20-week-old males, n = 6 per group). E: Insulin tolerance test (ITT). Blood glucose levels were...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2017;66(2):407–417
Published: 08 November 2016
... with vehicle or axitinib. L: H-E staining of fatty liver from mice treated with vehicle or axitinib. *P < 0.05, **P < 0.01, compared with control group (n = 9). IGTT, intraperitoneal glucose tolerance test; ITT, insulin tolerance test; NC, nontreated control; ND...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2007;56(8):1986–1998
Published: 01 August 2007
...@fcm.unicamp.br 18 5 2007 14 11 2006 DIABETES 2007 CLS, crown-like structure ELISA, enzyme-linked immunosorbent assay FFA, free fatty acid HFD, high-fat diet IGTT, intraperitoneal glucose tolerance test IκBα, inhibitor of nuclear factor-κB IKKβ, IκB kinase complex IL, interleukin...
Journal Articles
Journal: Diabetes
Diabetes 2003;52(6):1441–1448
Published: 01 June 2003
... AGE; ELISA, enzyme-linked immunosorbent assay; GALT, gut-associated lymphoid tissue; H&E, hematoxylin and eosin; H-AGE, high-AGE diet; IFN, interferon; IGTT, intraperitoneal glucose tolerance test; IL, interleukin; L-AGE, low-AGE diet; MG, methylglyoxal; PI, proinsulin; pLy, pancreatic lymphocytes...
Journal Articles
Journal: Diabetes
Diabetes 2013;62(5):1527–1536
Published: 16 April 2013
...FIG. 7. Intraperitoneal glucose tolerance tests (IGTTs) after exendin-4 administration. Overnight-fasted β-caPKA and wild-type (WT) littermate control mice were administered exendin-4 15 min before receiving an 2 g/kg i.p. glucose bolus. Plasma insulin was measured over the subsequent 20 min (0...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2012;61(10):2534–2545
Published: 13 September 2012
... administration. The peripheral response to glucose was evaluated by the intraperitoneal glucose tolerance test (IGTT) 32 days after the first ADMSC injection. The glucose (1.5 mg/g body wt) was administrated intraperitoneally in 12-h fasting mice, and blood glucose levels were determined before and 15, 30...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2005;54(6):1717–1725
Published: 01 June 2005
... glucose concentration was measured in samples taken at 0, 30, 60, 90, and 120 min after the glucose bolus. Insulin tolerance test (ITT) was performed on 6-h fasting mice by intraperitoneal injection of 1 unit/kg body wt human insulin (Sigma Aldrich, St. Louis, MO). Blood glucose concentration...
Journal Articles
Journal: Diabetes
Diabetes 2014;63(11):3626–3636
Published: 13 October 2014
... thermometer (Physitemp, Clifton, NJ). Hyperinsulinemic-euglycemic clamps were performed as described ( 19 ). An intraperitoneal glucose tolerance test (IGTT) was performed on conscious mice after 12-h fasting and an intraperitoneal injection with 20% glucose in saline (2 g/kg total body weight...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2013;62(7):2338–2346
Published: 14 June 2013
... (repeated measurements ANOVA). At least six mice were studied for each genotype. Graphs illustrate one of three independent studies all yielding similar results. Intraperitoneal glucose tolerance tests (IGTTs) conducted after 0, 4, and 8 weeks revealed that HFD-fed Neu1-deficient mice develop glucose...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2005;54(8):2314–2319
Published: 01 August 2005
... and euglycemic and hyperglycemic clamps in fat-fed mice exposed to high or low dietary AGE. A: Intravenous glucose tolerance test (IGTT) was performed at 6 months of study in subgroups of mice (n = 5 per group): control (C57/BL6 mice, ▵), HAGE-HF (▪), LAGE-HF (▴), and db/db (□). Data...