1-20 of 606 Search Results for

pi-phosphatidylinositol

Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Images
High resolution/accurate MS lipidomic analysis of ARPE-19 plasma membrane a...
Published: 23 January 2018
Figure 4 High resolution/accurate MS lipidomic analysis of ARPE-19 plasma membrane and TJ fractions. Negative ion Orbitrap high resolution/accurate MS of ARPE-19 plasma membrane (A) and ARPE-19 TJ lipid extracts (B) at 100,000 resolution. Sphingolipids and phosphatidylcholine lipids were detected as [M+HCO2−H] ions under the analytical conditions used. The indicated region was original magnified ×5 to enhance spectral detail. C: Quantitation of ceramide molecular species observed in ARPE-19 plasma membrane and TJ fractions. The inset indicates putative VLCFA-containing AcylCer species. HexCer, hexosylceramide; LacCer, lactosylceramide; PC, phosphatidylcholine; PI, phosphatidylinositol; SM, sphingomyelin. Figure 4. High resolution/accurate MS lipidomic analysis of ARPE-19 plasma membrane and TJ fractions. Negative ion Orbitrap high resolution/accurate MS of ARPE-19 plasma membrane (A) and ARPE-19 TJ lipid extracts (B) at 100,000 resolution. Sphingolipids and phosphatidylcholine lipids were detected as [M+HCO2−H]− ions under the analytical conditions used. The indicated region was original magnified ×5 to enhance spectral detail. C: Quantitation of ceramide molecular species observed in ARPE-19 plasma membrane and TJ fractions. The inset indicates putative VLCFA-containing AcylCer species. HexCer, hexosylceramide; LacCer, lactosylceramide; PC, phosphatidylcholine; PI, phosphatidylinositol; SM, sphingomyelin. More
Images
Lipid accumulation/imbalance and ER stress in ASKO mice. <em>A</em>...
Published: 14 June 2014
Figure 8 Lipid accumulation/imbalance and ER stress in ASKO mice. A: Relative mRNA levels of sphingolipid metabolism pathway genes in Epi-WAT from 6-month-old WT and ASKO mice. Sptlc3, serine palmitoyltransferase, long chain base subunit 3; Sgpp1, sphingosine-1-phosphate phosphatase 1; Acer3, alkaline ceramidase 3; Gla, galactosidase-α. Values are expressed as mean ± SEM, n = 5. Lipidomic analysis of Epi-WAT (B–E) and BAT (F–H) from 6-month-old WT and ASKO mice using liquid chromatography mass spectroscopy. Different lipid groups (B and F) and different classes of Cer (C, D, G, and H) and TAG (E) are compared. D and H: Sphingolipids are the sum of all ceramide and sphingomyelin species. Values are expressed as mean ± SEM, n = 4. *P < 0.05, **P < 0.01, ***P < 0.001 for ASKO vs. WT. Cer, ceramide; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin. I: Representative Western blot images for the indicated proteins and phosphoproteins related to ER stress in Epi-WAT from 10-month-old WT and ASKO mice. Figure 8. Lipid accumulation/imbalance and ER stress in ASKO mice. A: Relative mRNA levels of sphingolipid metabolism pathway genes in Epi-WAT from 6-month-old WT and ASKO mice. Sptlc3, serine palmitoyltransferase, long chain base subunit 3; Sgpp1, sphingosine-1-phosphate phosphatase 1; Acer3, alkaline ceramidase 3; Gla, galactosidase-α. Values are expressed as mean ± SEM, n = 5. Lipidomic analysis of Epi-WAT (B–E) and BAT (F–H) from 6-month-old WT and ASKO mice using liquid chromatography mass spectroscopy. Different lipid groups (B and F) and different classes of Cer (C, D, G, and H) and TAG (E) are compared. D and H: Sphingolipids are the sum of all ceramide and sphingomyelin species. Values are expressed as mean ± SEM, n = 4. *P < 0.05, **P < 0.01, ***P < 0.001 for ASKO vs. WT. Cer, ceramide; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin. I: Representative Western blot images for the indicated proteins and phosphoproteins related to ER stress in Epi-WAT from 10-month-old WT and ASKO mice. More
Images
Association and predictive modeling of plasma lipids in detecting AF in ADV...
Published: 28 October 2020
Figure 2 Association and predictive modeling of plasma lipids in detecting AF in ADVANCE subset 1 (n = 3,772) (A and C), and patients in whom AF developed in the future (ADVANCE subset 2; n = 2,238) (B and D). A and B: Logistic regression model of individual lipid species against prevalence of AF at baseline (subset 1, AF Baseline) and future AF (subset 2, AF Future) adjusting for 12 covariates (namely, age, weight, height, systolic blood pressure, diastolic blood pressure, total cholesterol, HDL cholesterol, triglycerides, myocardial infarction, antihypertensive medication used, current smoking status, and hospital admission for heart failure). Odds ratios and 95% CIs are shown. Lipid levels in red (A) and blue (B) were significant (P < 0.05) after applying the Benjamini-Hochberg correction. C and D: Logistic regression models used to determine improvement in the C-statistic to detect or predict the incidence of AF by adding lipids to the base model, consisting of the 12 covariates. Logistic regression models were developed using forward stepwise feature selection by Akaike information criterion reduction. Analysis was conducted in a fivefold cross-validated framework (repeated 200 times). Ranked lipids are shown sequentially as features added to the base model. CE, cholesteryl ester; Cer, ceramide; DG, diacylglycerol; dhCer, dihydroceramide; GM3, monosialodihexosylganglioside; HexCer, monohexosylceramide; Hex2Cer, dihexosylceramide; Hex3Cer, trihexosylceramide; LPC, lysophosphatidylcholine; LPC(O), lysoalkylphosphatidylcholine; LPI, lysophosphatidylinositol; PC, phosphatidylcholine; PC(O), alkylphosphatidylcholine; PC(P), alkenylphosphatidylcholine; PE, phosphatidylethanolamine; PE(O), alkylphosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; SM, sphingomyelin; TG, triacylglycerol. Figure 2. Association and predictive modeling of plasma lipids in detecting AF in ADVANCE subset 1 (n = 3,772) (A and C), and patients in whom AF developed in the future (ADVANCE subset 2; n = 2,238) (B and D). A and B: Logistic regression model of individual lipid species against prevalence of AF at baseline (subset 1, AF Baseline) and future AF (subset 2, AF Future) adjusting for 12 covariates (namely, age, weight, height, systolic blood pressure, diastolic blood pressure, total cholesterol, HDL cholesterol, triglycerides, myocardial infarction, antihypertensive medication used, current smoking status, and hospital admission for heart failure). Odds ratios and 95% CIs are shown. Lipid levels in red (A) and blue (B) were significant (P < 0.05) after applying the Benjamini-Hochberg correction. C and D: Logistic regression models used to determine improvement in the C-statistic to detect or predict the incidence of AF by adding lipids to the base model, consisting of the 12 covariates. Logistic regression models were developed using forward stepwise feature selection by Akaike information criterion reduction. Analysis was conducted in a fivefold cross-validated framework (repeated 200 times). Ranked lipids are shown sequentially as features added to the base model. CE, cholesteryl ester; Cer, ceramide; DG, diacylglycerol; dhCer, dihydroceramide; GM3, monosialodihexosylganglioside; HexCer, monohexosylceramide; Hex2Cer, dihexosylceramide; Hex3Cer, trihexosylceramide; LPC, lysophosphatidylcholine; LPC(O), lysoalkylphosphatidylcholine; LPI, lysophosphatidylinositol; PC, phosphatidylcholine; PC(O), alkylphosphatidylcholine; PC(P), alkenylphosphatidylcholine; PE, phosphatidylethanolamine; PE(O), alkylphosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; SM, sphingomyelin; TG, triacylglycerol. More
Journal Articles
Journal: Diabetes
Diabetes 1997;46(1):17–22
Published: 01 January 1997
...) fusion gene. Wortmannin, an inhibitor of 1-phosphatidylinositol 3- kinase (PI 3-kinase), blocks the inhibition of glucocorticoid and cAMP-induced PEPCK gene transcription by insulin; however, it has no effect on the inhibition elicited by oxidative or chemical stress. Thus, the mechanism(s) used...
Meeting Abstracts
Journal: Diabetes
Diabetes 1998;47(9):1494–1500
Published: 01 September 1998
... of troglitazone contains vitamin E. Accordingly, we studied the effect of troglitazone, pioglitazone, and vitamin E on thrombin-induced platelet aggregation, metabolism of phosphoinositide, protein phosphorylation, protein kinase C (PKC)-alpha and -beta, and phosphatidylinositol (PI) 3-kinase activation in vitro...
Journal Articles
Journal: Diabetes
Diabetes 1997;46(3):524–527
Published: 01 March 1997
...Marie Björnholm; Yuichi Kawano; Mikael Lehtihet; Juleen R Zierath We examined the effect of physiological hyperinsulinemia on insulin receptor substrate-1 (IRS-1) tyrosine phosphorylation and phosphatidylinositol (PI) 3-kinase activity in skeletal muscle from six lean–to–moderately obese NIDDM...
Journal Articles
Journal: Diabetes
Diabetes 1997;46(2):215–223
Published: 01 February 1997
... with [3H]-ATB bismannose was reduced by 50% (P < 0.001). Insulinreceptor substrate 1 (IRS-1) associated phosphatidylinositol (PI) 3-kinase activity stimulated by insulin was also reduced by 36% (P < 0.001), and expression of p85 and p110b subunits of PI 3-kinase...
Meeting Abstracts
Journal: Diabetes
Diabetes 2000;49(10):1740–1743
Published: 01 October 2000
...M Kossila; M Sinkovic; P Kärkkäinen; M O Laukkanen; R Miettinen; J Rissanen; P Kekäläinen; J Kuusisto; S Ylä-Herttuala; M Laakso Phosphatidylinositol (PI) 3-kinase is a key signaling molecule in insulin-stimulated glucose transport. Therefore, we investigated the catalytic subunit p110beta...
Journal Articles
Journal: Diabetes
Diabetes 1990;39(12):1561–1568
Published: 01 December 1990
... platelet aggregation rates (DM-B group), and 8 age-matched healthy control subjects. The mass and specific radioactivity of phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol (PI), and phosphatidic acid (PA) in 32P-labeled...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(2):479–483
Published: 01 February 2002
..., phosphotyrosine-associated phosphatidylinositol (PI) 3-kinase activity. Because the increase in PI 3-kinase activity cannot be explained by increased insulin receptor substrate (IRS)-1 signaling, the present study examined whether this effect is mediated by enhanced IRS-2 signaling. In wild-type (WT) mice...
Journal Articles
Journal: Diabetes
Diabetes 1995;44(11):1345–1348
Published: 01 November 1995
... was completely blocked in the presence of dantrolene (P < 0.01). Inhibition of phosphatidylinositol (PI) 3-kinase by wortmannin (500 nmol/l) had no effect on the activation of glucose transport by hyperglycemia, whereas the insulin-stimulated glucose transport was completely abolished (P < 0.001...
Journal Articles
Journal: Diabetes
Diabetes 1998;47(2):179–185
Published: 01 February 1998
..., and phosphatidylinositol (PI) 3-kinase activity (using PI as substrate) and mitogen-activated protein kinase (MAPK) activity were assayed in cell lysates. Englitazone increased 2DG uptake in a concentrationdependent (10–100 μmol/l) manner by up to sixfold, and preincubation with englitazone significantly enhanced insulin...
Images
Proposed model. MMP, matrix metalloproteinase; EGF, epidermal growth factor...
Published: 13 November 2011
FIG. 6. Proposed model. MMP, matrix metalloproteinase; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor; PI-3K, phosphatidylinositol-3 kinase. (A high-quality color representation of this figure is available in the online issue.) FIG. 6. Proposed model. MMP, matrix metallopro... More
Journal Articles
Journal: Diabetes
Diabetes 1996;45(7):869–875
Published: 01 July 1996
...Dominique Jullien; Stanley J Heydrick; Nadine Gautier; Emmanuel Van Obberghen; Yannick Le Marchand-Brustel Insulin and IGF-I induced a similar stimulation of glucose transport in isolated soleus muscle. These actions require phosphatidylinositol (PI) 3-kinase activation since the PI 3-kinase...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(11):2780–2789
Published: 01 November 2007
... LTCC, l-type Ca2+ channel LVEDD, left ventricular end-diastolic diameter LVESD, left ventricular end-systolic diameter PI, phosphatidylinositol PI(3,4,5)P3, PI 3,4,5-trisphosphate PI(4,5)P2, PI 4,5-bisphosphate V1/2, membrane potential...
Journal Articles
Journal: Diabetes
Diabetes 1997;46(11):1775–1781
Published: 01 November 1997
.... Concomitant insulin stimulation (three- to six-fold [P < 0.05]) of thigh glucose clearance, muscle insulin receptor tyrosine kinase (IRTK), insulin receptor substrate-1 (IRS-1) tyrosine phosphorylation, and IRS-1-associated phosphatidylinositol 3-kinase (PI 3-kinase) was observed in the rested leg...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(2):443–448
Published: 01 February 2002
...Young-Bum Kim; Theodore P. Ciaraldi; Alice Kong; Dennis Kim; Neelima Chu; Pharis Mohideen; Sunder Mudaliar; Robert R. Henry; Barbara B. Kahn Insulin stimulation of phosphatidylinositol (PI) 3-kinase activity is defective in skeletal muscle of type 2 diabetic individuals. We studied the impact...
Meeting Abstracts
Journal: Diabetes
Diabetes 1998;47(10):1562–1569
Published: 01 October 1998
... of insulin on 2-deoxyglucose uptake activity was reduced in oxidized cells and could be attributed to GLUT1 translocation. Insulin stimulation of insulin receptor substrate (IRS) 1 tyrosine phosphorylation and the association of IRS-1 with phosphatidylinositol (PI) 3-kinase were not impaired by oxidative...
Journal Articles
Journal: Diabetes
Diabetes 2005;54(2):361–366
Published: 01 February 2005
...) at the level of insulin receptor phosphorylation, insulin receptor substrate phosphorylation, and downstream signaling elements such as phosphatidylinositol (PI) 3-kinase, AKT, and mitogen-activated protein kinase. C57BL/6 mice were injected with insulin glulisine or regular insulin and Western blot analysis...
Journal Articles
Journal: Diabetes
Diabetes 2002;51(8):2387–2394
Published: 01 August 2002
...Hiroyuki Hori; Toshiyasu Sasaoka; Hajime Ishihara; Tsutomu Wada; Shihou Murakami; Manabu Ishiki; Masashi Kobayashi SH-2-containing inositol 5′-phosphatase 2 (SHIP-2) is a physiologically important lipid phosphatase that functions to hydrolyze phosphatidylinositol (PI) 3-kinase product PI(3,4,5)P3...